[1]张 迪,王君君,吕承秀,等.山东淄博地区儿童感染肺炎支原体的分子分型和耐药性研究[J].现代检验医学杂志,2026,41(01):116-120.[doi:10.3969/j.issn.1671-7414.2026.01.022]
　ZHANG Di,WANG Junjun,LYU Chengxiu,et al.Molecular Typing and Antimicrobial Resistance of Mycoplasma Pneumoniae Infection in Children in Zibo, Shandong[J].Journal of Modern Laboratory Medicine,2026,41(01):116-120.[doi:10.3969/j.issn.1671-7414.2026.01.022]
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山东淄博地区儿童感染肺炎支原体的分子分型和耐药性研究()

《现代检验医学杂志》[ISSN:/CN:]

卷:
第41卷
期数:
2026年01期
页码:
116-120
栏目:
论著
出版日期:
2026-01-15

文章信息/Info

Title:
Molecular Typing and Antimicrobial Resistance of Mycoplasma Pneumoniae Infection in Children in Zibo, Shandong
文章编号:
1671-7414（2026）01-116-05
作者:
张 迪a，b，王君君a，b，吕承秀a，b，范艳娇c，李 庆a，b
淄博市第一医院a.检验科；b.淄博市分子免疫检验医学重点实验室；c. 儿科，山东淄博 255200
Author(s):
ZHANG Dia，b, WANG Junjuna，b, LYU Chengxiua，b, FAN Yanjiaoc, LI Qinga，b
a. Department of Clinical Laboratory;b. Zibo Key Laboratory of Molecular Immunology of Laboratory Medicine; c. Department of Pediatrics, Zibo First Hospital, Shangdong Zibo 255200, China
关键词:
肺炎支原体; 分子分型; 耐药性; 儿童; 山东淄博
分类号:
R375.2；R446.5
DOI:
10.3969/j.issn.1671-7414.2026.01.022
文献标志码:
A
摘要:
目的?分析山东淄博地区儿童感染肺炎支原体（MP）的分子分型和耐药性，为临床治疗提供可靠的参考依据。方法?选取2023年11月~2024年7月于淄博市第一医院就诊的49例肺炎支原体肺炎患儿作为研究对象，采用巢式聚合酶链反应检测患儿痰标本中MPP1基因、MP23S核糖体RNA（23SrRNA）结构域V、MP核糖体蛋白基因L4和L22，同时对痰标本进行MP培养及药敏试验，分析儿童感染MP的分子分型和耐药性。结果患儿痰标本中MPP1基因型以MPP1-1型为主，占83.67%（41/49）。MP23SrRNA结构域V位点突变检出率为95.92%（47/49），突变类型均为A2063G；MP核糖体蛋白基因L4和L22位点突变检出率为16.33%（8/49）。MP对红霉素、依托红霉素、克拉霉素、罗红霉素、克林霉素以及阿奇霉素的耐药率均高于80.00%，MP对米诺环素、多西环素、环丙沙星、莫西沙星、左氧氟沙星、加替沙星耐药率均为0.00%。MP23SrRNA结构域V位点突变的菌株对依托红霉素、红霉素、克拉霉素、罗红霉素、阿奇霉素以及克林霉素的耐药率均高于核糖体蛋白基因突变的菌株，差异具有统计学意义（χ2=20.451～43.000，均P<0.05）。MPP1-1型菌株对红霉素、克拉霉素、罗红霉素、阿奇霉素、克林霉素耐药性均高于MPP1-2型菌株，差异具有统计学意义（χ2=7.774～22.322，均P<0.05）。结论山东淄博地区肺炎支原体感染流行菌株以MPP1-1型为主，MP23SrRNA结构域VA2063G位点突变检出率高。MPP1-1型菌株对常用大环内酯类抗生素的耐药率高于MPP1-2型菌株。
Abstract:
Objective To analyze the molecular typing and antimicrobial resistance of Mycoplasma pneumoniae (MP) infec-tion in children in Zibo of Shandong, and provide a reliable reference for clinical treatment. Methods A total of 49 hospital-ized children who visited the Zibo First Hospital from November 2023 to July 2024 were selected as the research subjects. The nested polymerase chain reaction method was used to detect the MP P1 gene, MP23S ribosomal RNA (23S rRNA) domain V, MP ribosomal protein genes L4 and L22 in sputum specimens. At the same time, MP culture and antimicrobial sensitivity tests were performed on sputum specimens to analyze the molecular typing and antimicrobial resistance of children infect-ed with MP. Results The MP P1 typing in the sputum specimens of the children was mainly MPP1-1 type, accounting for 83.67% (41/49). The detection rate of MP 23S rRNA domain V site mutation was 95.92% (47/49), and the mutation type was A2063G; the detection rate of MP ribosomal protein gene L4 and L22 site mutation was 16.33% (8/49). The resistance rates of MP to erythromycin, erythromycin estolate, clarithromycin, roxithromycin, clindamycin and azithromycin were all higher than 80.00%, and the resistance rates of MP to minocycline, doxycycline, ciprofloxacin, moxifloxacin, levofloxacin and gati-floxacin were all 0.00%. The resistance rates of strains with mutations in the V site of the MP23SrRNA domain to erythro-mycin, erythromycin, clarithromycin, roxithromycin, clindamycin and azithromycin were all higher than those of strains with mutations in the ribosomal protein gene, and the differences were statistically significant (χ2=20.451~43.000, all P<0.05).The resistance of MP P1-1 type strains to erythromycin, clarithromycin, roxithromycin, azithromycin and clindamycin was higher than that of P1-2 type strains, and the differences were statistically significant (χ2=7.774~22.322, all P<0.05). Conclusions The prevalent strains of MP infection in Zibo, Shandong are mainly MP P1-1 type, with a high detection rate of A2063G mutation in the MP 23S rRNA domain V. The resistance rate of MP P1-1 type strains to commonly used macrolide antibiotics is higher than that of MP P1-2 type strains.

参考文献/References:

[1] LI Z J, ZHANG H Y, REN L L, et al. Etiological and ep-idemiological features of acute respiratory infections in China[J]. Nature Communications, 2021, 12(1): 5026.
[2] KENRI T, SUZUKI M, SEKIZUKA T, et al. Periodic genotype shifts in clinically prevalent Mycoplasma pneumoniae strains in Japan[J]. Frontiers in Cellular and Infection Microbiology, 2020, 10: 385.
[3] WANG G T, WU P, TANG R, et al. Global prevalence of resistance to macrolides in Mycoplasma pneumoniae : a systematic review and meta-analysis[J]. The Journal of Antimicrobial Chemotherapy, 2022, 77(9): 2353-2363.
[4] 冯华,薛洪刚,徐玉秀.难治性肺炎支原体肺炎患儿血清长链非编码RNA肺腺癌转移相关转录因子1和烟酰胺核苷酸反义转氢酶RNA1检测的临床意义[J].现代检验医学杂志,2022,37(4):7-12, 164. FENG H, XUE H G, XU Y X. Clinical significance of serumlong non-coding RNA metastasis-associated lung adenocarcinoma transcript 1, nicotinamide nucleotide transhydrogenase-antisense RNA1 in children with refrac-tory Mycoplasma pneumoniae pneumonia[J]. Journal of Modern Laboratory Medicine, 2022, 37(4): 7-12, 164.
[5] 王欣,赵德育.儿童肺炎支原体分子流行病学研究进展[J].国际儿科学杂志,2021,48(7):488-491. WANG X, ZHAO D Y. Progress of molecular epidemi-ology of Mycoplasma pneumoniae in children[J]. Inter-national Journal of Pediatrics, 2021, 48(7): 488-491.
[6] DUMKE R, L?CK P C, NOPPEN C, et al. Culture-in-dependent molecular subtyping of Mycoplasma pneu-moniae in clinical samples[J]. Journal of Clinical Mi-crobiology, 2006, 44(7): 2567-2570.
[7] KENRI T, YAMAZAKI T, OHYA H, et al. Genotyping of Mycoplasma pneumoniae strains isolated in Japan during 2019 and 2020: spread of p1 gene type 2c and 2j variant strains[J]. Frontiers in Microbiology, 2023, 14: 1202357.
[8] WANG N, ZHANG H, YIN Y H, et al. Antimicrobi-al susceptibility profiles and genetic characteristics of Mycoplasma pneumoniae in Shanghai, China, from 2017 to 2019[J]. Infection and Drug Resis-tance,2022,15:4443-4452.
[9] KUITUNEN I, ARTAMA M, HAAPANEN M, et al. Respiratory virus circulation in children after relaxation of COVID-19 restrictions in fall 2021-A nationwide register study in Finland[J]. Journal of Medical Virolo-gy, 2022, 94(9): 4528-4532.
[10] MEYER SAUTEUR P M, BEETON M L, ULDUM S A, et al. Mycoplasma pneumoniae detections before and during the COVID-19 pandemic: results of a glob-al survey, 2017 to 2021[J]. Euro Surveillance: Bulletin Europe?en Sur Les Maladies Transmissibles = European Communicable Disease Bulletin, 2022, 27(19): 2100746.
[11] EDENS C, CLOPPER B R, DEVIES J, et al. Notes from the field: reemergence of Mycoplasma pneumoniae in-fections in children and adolescents after the COVID-19 pandemic, United States, 2018-2024[J]. Morbidity and Mortality Weekly Report, 2024, 73(7): 149-151.
[12] CHEN Y J, JIA X Y, GAO Y G, et al. Increased mac-rolide resistance rate of Mycoplasma pneumoniae correlated with epidemic in Beijing, China in 2023[J]. Frontiers in Microbiology, 2024, 15: 1449511.
[13] 张丽侠,李荣利,李萍,等.小儿肺炎支原体感染临床特征及危险因素分析[J].中国病原生物学杂志, 2022,17(3):314-316. ZHANG L X, LI R L, LI P, et al. Investigation on clinical status and risk factors of Mycoplasma pneumoniae in chil-dren[J]. Journal of Pathogen Biology, 2022, 17(3): 314-316.
[14] RODMAN BERLOT J, KRIVEC U, MRVI?T, et al. My-coplasma pneumoniae P1 genotype indicates severity of lower respiratory tract infections in children[J]. Journal of Clinical Microbiology, 2021, 59(8): e0022021.
[15] GONG C, HUANG F, SUO L D, et al. Increase of respira-tory illnesses among children in Beijing, China, during the autumn and winter of 2023[J]. Euro Surveillance : Bulle-tin Europe?en Sur Les Maladies Transmissibles = European Communicable Disease Bulletin, 2024, 29(2): 2300704.
[16] KIM K, JUNG S, KIM M, et al. Global trends in the proportion of macrolide-resistant Mycoplasma pneu-moniae infections: a systematic review and meta-analy-sis[J]. JAMA Network Open, 2022, 5(7): e2220949.
[17] JIANG Y, DOU H W, XU B, et al. Macrolide resistance of Mycoplasma pneumoniae in several regions of China from 2013 to 2019[J]. Epidemiology and Infection, 2024, 152: e75.
[18] GUO P B, MEI S Y, WANG Y H, et al. Molecular typing of Mycoplasma pneumoniae and its correlation with mac-rolide resistance in children in Henan of China[J]. Indian Journal of Medical Microbiology, 2023, 46: 100435.
[19] DEKYI, XIAO Y J, WANG X, et al. Predominance of a2063g mutant strains in the Mycoplasma pneumoniae epidemic in children: A clinical and epidemiological study in 2023 in Wuhan, China[J]. International Journal of In-fectious Diseases, 2024, 145: 107074.
[20] JIANG Y, KANG H L, DOU H W, et al. Comparative genomic sequencing to characterize Mycoplasma pneu-moniae genome, typing, and drug resistance[J]. Micro-biology Spectrum, 2024, 12(8): e0361523.
[21] WANG N, XU X G, XIAO L, et al. Novel mechanisms of macrolide resistance revealed by in vitro selection and ge-nome analysis in Mycoplasma pneumoniae[J]. Frontiers in Cellular and Infection Microbiology, 2023, 13: 1186017.
[22] ISHIGURO N, SATO R, KIKUTA H, et al. P1 gene of Mycoplasma pneumoniae isolated from 2016 to 2019 and relationship between genotyping and macrolide resistance in Hokkaido,Japan[J]. Journal of Medical Microbiology, 2021, 70(6): 001365.

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备注/Memo

备注/Memo:
基金项目：山东第二医科大学附属医院（教学医院）科研发展基金资助项目（2023FYM062）。
作者简介：张迪(1991-），女，硕士，主管检验师，研究方向：病原学检验，E-mail：1031601788@qq.com。
通讯作者：吕承秀（1987-），男，硕士，副主任技师，研究方向：病原体分子生物学检验，E-mail：lvchengxiu870901@126.com。
更新日期/Last Update: 2026-01-15