[1]马 瑶,李相金,李 睿,等.结核性胸膜炎患者胸腔积液中PTX3、NAMPT和ADA表达水平及临床价值研究[J].现代检验医学杂志,2026,41(02):134-140.[doi:10.3969/j.issn.1671-7414.2026.02.023]
　MA Yao,LI Xiangjin,LI Rui,et al.Expression Levels and Clinical Significance of PTX3，NAMPT and ADA in Pleural Effusions of Patients with Tuberculous Pleurisy[J].Journal of Modern Laboratory Medicine,2026,41(02):134-140.[doi:10.3969/j.issn.1671-7414.2026.02.023]
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结核性胸膜炎患者胸腔积液中PTX3、NAMPT和ADA表达水平及临床价值研究()

《现代检验医学杂志》[ISSN:/CN:]

卷:
第41卷
期数:
2026年02期
页码:
134-140
栏目:
论著
出版日期:
2026-03-15

文章信息/Info

Title:
Expression Levels and Clinical Significance of PTX3，NAMPT and ADA in Pleural Effusions of Patients with Tuberculous Pleurisy
文章编号:
1671-7414（2026）02-134-07
作者:
马 瑶a，李相金b，李 睿c，张许林a，吴桂辉a
成都市公共卫生临床医疗中心 a. 结核科；b. 检验科；c. 内科，成都 610011
Author(s):
MA Yaoa, LI Xiangjinb, LI Ruic, ZHANG Xulina, WU Guihuia
a. Department of Tuberculosis; b. Department of Labo-ratory; c. Department of Internal Medicine, Chengdu Public Health Clinical Medical Center, Chengdu 610011, China
关键词:
结核性胸膜炎; 正五聚蛋白3; 烟酰胺磷酸核糖基转移酶; 腺苷脱氨酶
分类号:
R521.7；R446.19
DOI:
10.3969/j.issn.1671-7414.2026.02.023
文献标志码:
A
摘要:
目的?探讨结核性胸膜炎(TBP)患者胸腔积液中正五聚蛋白3(PTX3)、烟酰胺磷酸核糖基转移酶(NAMPT)和腺苷脱氨酶(ADA)表达水平及临床价值。方法选取2022年1月~2024年1月成都市公共卫生临床医疗中心收治的TBP患者180例(TBP组)和同期收治的类肺炎性胸腔积液、漏出性胸腔积液患者90例(非TBP组),根据预后将TBP患者分为不良预后组(n=52)和良好预后组(n=128)。采用酶联免疫吸附法(ELISA)检测胸腔积液中PTX3、NAMPT和ADA表达水平。通过多因素非条件Logistic回归分析胸腔积液中PTX3、NAMPT、ADA表达水平与TBP患者不良预后的关系;受试者操作特征(ROC)曲线分析胸腔积液中PTX3、NAMPT、ADA表达水平对TBP的诊断效能和不良预后的预测效能。结果TBP组胸腔积液中PTX3(3.10±0.45ng/ml)、NAMPT(2.94±0.35ng/ml)、ADA(41.64±10.66U/L)表达水平高于非TBP组(2.40±0.47ng/ml、2.46±0.29ng/ml、29.49±6.38U/L),差异具有统计学意义(t=11.846、11.187、11.664,均P<0.05)。180例TBP患者预后不良率为28.89%(52/180)。不良预后组胸腔积液中PTX3(3.45±0.30ng/ml)、NAMPT(3.22±0.31ng/ml)、ADA(50.12±11.24U/L)表达水平高于良好预后组(2.96±0.42ng/ml、2.82±0.30ng/ml、38.19±8.25U/L),差异具有统计学意义(t=7.623、8.024、6.931,均P<0.05)。糖尿病(OR=3.365)、PTX3高(OR=1.382)、NAMPT高(OR=1.745)、ADA高(OR=1.115)为TBP患者不良预后的独立危险因素(Waldχ2=5.264~19.911,均P<0.05)。胸腔积液中PTX3、NAMPT、ADA表达水平联合诊断TBP的曲线下面积(AUC)为0.963,大于胸腔积液中PTX3、NAMPT、ADA表达水平单独诊断的0.874、0.860、0.844,差异具有统计学意义(Z=4.578、5.197、5.491,均P<0.05)。胸腔积液中PTX3、NAMPT、ADA表达水平预测TBP患者不良预后的AUC为0.938,大于胸腔积液中PTX3、NAMPT、ADA表达水平单独预测的0.839、0.825、0.806,差异具有统计学意义(Z=3.316、3.767、4.231,均P<0.05)。结论胸腔积液中PTX3、NAMPT、ADA表达水平升高与TBP患者预后不良密切相关,二者联合对TBP的诊断效能和预后预测效能较高,可能成为TBP患者诊断和预后预测的标志物。
Abstract:
Objective To investigate the expression levels and clinical value of pentraxin 3 (PTX3)、nicotinamide phosphoribosyl transferase (NAMPT) and adenosine deaminase (ADA) in pleural effusion of patients with tuberculous pleurisy (TBP). Methods A total of 180 patients with TBP (TBP group) and 90 patients with parapneumonic pleural effusion or transudative pleural effu-sion (non-TBP group) admitted to Chengdu Public Health Clinical Medical Center from January 2022 to January 2024 were en-rolled. TBP patients were further divided into poor prognosis group(n=52) and favorable prognosis group(n=128) based on clini-cal outcomes. The expression levels of PTX3, NAMPT and ADA in pleural effusions were detected using enzyme-linked immunosorbent assay (ELISA). The relationship between the expression levels of PTX3, NAMPT and ADA in pleural effusion and the poor prognosis of TBP patients was analyzed by multivariate unconditional Logistic regression. ROC curve was used to analyze the diagnostic efficiency of PTX3, NAMPT and ADA expression levels in pleural effusion for TBP and the predictive ef-ficiency for poor prognosis in TBP patients. Results The expression levels of PTX3 (3.10 ± 0.45ng/ml), NAMPT (2.94 ± 0.35ng/ml) and ADA (41.64 ± 10.66U/L) in pleural effusion of TBP group were higher than those in the non-TBP group (2.40 ± 0.47ng/ml, 2.46 ± 0.29ng/ml, 29.49 ± 6.38U/L), and the differences were statistically significant (t= 11.846, 11.187, 11.664,all P< 0.05). The poor prognosis rate of 180 patients with TBP was 28.89 % (52/180).The expression levels of PTX3 (3.45 ± 0.30ng/ml), NAMPT (3.22 ± 0.31ng/ml) and ADA (50.12 ± 11.24U/L) in pleural effusion in the poor prognosis group were higher than those in the favorable prognosis group (2.96 ± 0.42ng/ml, 2.82 ± 0.30ng/ml, 38.19 ± 8.25U/L), and the differences were statistically significant (t= 7.623, 8.024, 6.931, all P< 0.05). Diabetes (OR= 3.365), high PTX3 (OR = 1.382), high NAMPT (OR = 1.745) and high ADA (OR = 1.115) were independent risk factors for poor prognosis in patients with TBP (Wold χ2=5.264~ 19.911,all P< 0.05).The combined diagnostic area under the curve (AUC) for PTX3, NAMPT and ADA expression levels in pleural effusion was 0.963, exceeding the individual AUCs of 0.874, 0.860 and 0.844 for PTX3, NAMPT and ADA alone, respec-tively (Z= 4.578, 5.197, 5.491, all P< 0.05). The AUC for predicting poor prognosis in TBP patients using PTX3, NAMPT and ADA expression levels in pleural effusion was 0.938, which was greater than the AUCs of 0.839, 0.825 and 0.806 for each mark-er alone , and the differences were statisticlly significant (Z= 3.316, 3.767, 4.231, all P< 0.05). Conclusions The increased ex-pression levels of PTX3, NAMPT and ADA in pleural effusion are closely related to the poor prognosis in TBP patients. The combined use of these markers has high diagnostic and prognostic predictive efficiency for TBP, potentially serving as biomark-ers for diagnosis and prognosis prediction in TBP patients.

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备注/Memo

备注/Memo:
基金项目：成都市医学科研课题（2022371）；四川省医学会（青年创新）科研课题（S22024）。
作者简介：马瑶(1982-)，女，本科，副主任医师，研究方向：呼吸病学、结核病，E-mail：mary_401@163.com。
通讯作者：吴桂辉（1969-），女，硕士研究生，主任医师，研究方向：结核病，E-mail：Wghwgh2584@sina.com。
更新日期/Last Update: 2026-03-15