[1]洪 俊,饶永彩.XN-9000血细胞分析仪筛查外周血中Sezary细胞的临床价值研究[J].现代检验医学杂志,2017,32(03):92-96.[doi:10.3969/j.issn.1671-7414.2017.03.025]
 HONG Jun,RAO Yong-cai.Performance Evaluation of Alarms and Parameters Generated by Hematology Analyzer during Detection of Circulating Sezary Cells of Peripheral Blood Smears[J].Journal of Modern Laboratory Medicine,2017,32(03):92-96.[doi:10.3969/j.issn.1671-7414.2017.03.025]
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XN-9000血细胞分析仪筛查外周血中Sezary细胞的临床价值研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年03期
页码:
92-96
栏目:
论著
出版日期:
2017-05-25

文章信息/Info

Title:
Performance Evaluation of Alarms and Parameters Generated by Hematology Analyzer during Detection of Circulating Sezary Cells of Peripheral Blood Smears
文章编号:
1671-7414(2017) 03-092-05
作者:
洪 俊1饶永彩2
武汉大学人民医院检验科,武汉 430060;
2.武汉生物制品所,武汉 4 30060
Author(s):
HONG Jun1RAO Yong-cai2
1.Department of Clinical Laboratory,Renmin Hospital of Wuhan U niversity,Wuhan 430060,China;
2.Wuhan Institute of Biological Products,Wuhan 430060,Ch ina
关键词:
Sezary综合征血细胞分析仪形态学
分类号:
R446.11
DOI:
10.3969/j.issn.1671-7414.2017.03.025
文献标志码:
A
摘要:
目的 评估采用XN-900形态报警和结构参数用于筛检外周 血中Sezary细胞的可行性和有效性。方法 分别采用光学显微镜,XN -9000血细胞分析仪和流式细胞术(FCM)对21例Sezary综合征患者(sezary syndrome,SS)和 三个对照组(皮肤T细胞淋巴瘤对照组48例、慢性淋巴组织增生性B细胞疾病(CLPD)对照组55 例、正常人对照组45例)进行检测,采用对照分析法对Sysmex xn-9000分析生成的淋巴细胞 结构参数(ly-x,ly-y,ly-z)及 “Blast/Abn Lympho(原始/异常淋巴)”报警进行对比 分析,采用受试者工作曲线(ROC)来确定ly-x和ly-z的最佳触发筛查Sezary细胞的临界值 ,对“Blast/Abn Lympho(原始/异常淋巴)”报警提示和结构参数(ly-x和ly-z)的各种组 合筛检规则的诊断效能指标进行评估。结果 Sezary综合征组淋巴细 胞结构参数ly-x和ly-z分别为89.80(87.55~91.52)和73.60(69.10~74.32),明显 高于皮肤T细胞淋巴瘤对照组、CLPD组和正常人对照组(U=37,40,44,55,38,41,均P <0.05);ly-x与Sezary细胞数量正相关,ly-z与大Sezary细胞存在正相关(r=0.248 ,0.198,P<0.05);采用ROC曲线确定ly-x和ly-z筛查Sezary细胞的临界值分别为≥8 6和≥68,将上述三个指标组合起来(即“报警Blast/Abn Lympho≥100,ly-x≥86且ly-z ≥68)的灵敏度和特异度分别为100%和92.1%,可获得最好的筛检Sezary细胞诊断效能。〖H T5”H〗结论 采用血细胞分析仪所产生的“Blast/Abn Lympho(原始/异常淋巴) ” 报警提示和结构参数 (ly-x,ly-z) 组合规则可在外周血中Sezary细胞筛检中获得较 高的特异度和敏感度,可用于疑似SS样本的复片筛检。
Abstract:
Objective To evaluate the performance alarms an d parameters generated by hematology analyzer during detection of circulating se zary cells of peripheral blood smears.Methods Blood samp les from patients with Sezary syndrome (n=21) were studied with Sysmex XN-9 000 analyzer,and compared to patients with benign or tumoral skin lesions (n =48) and patients with chronic lymphoproliferative B-cell diseases (n=55) and normal person (n=45) used as control.Results I n present series,the value of structural lymphoid parameters (ly-x and ly-z) and the alarm Blast/Abn Lympho were statistically higher in Sezary cases than in control cases (U=37,40,44,55,38,41,all P<0.05).Screening Sezary cells critical values of ly-x and ly-z determined by ROC curve were greater than or equal to 86 and was more than or equal to 68.The combination of above t hree indexes up (i.e.“alarm Blast/Abn Lympho>100,ly-x=86 and ly-z>68) sen sitivity and specificity respectively for 100% and 92.1%,in order to get the b est screening Sezary cells diagnostic efficiency.In addition,the value of ly- x was associated to the count of circulating Sezary cells and value of ly-z to the presence of large Sezary cells,both parameters described as prognostic fact ors.Conclusion The combination of alarm Blast/Abn Lympho and structural parameters (ly-x/ly-z/ly-y) may allow to define rule of blood slide review to screen circulating Sezary cells.

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备注/Memo

备注/Memo:

作者简介:洪 俊(1973-),男,博士,副主任技师,主要从事临 床免疫及血液学方面的研究,E-mail:wonderman1973@hotmail.com。
更新日期/Last Update: 2017-06-25