[1]张翠平,陈婷婷,王成,等.儿童原发性肾病综合征患者尿液外泌体miR-23b-3p水平变化及其临床意义[J].现代检验医学杂志,2019,34(01):1-4,9.[doi:10.3969/j.issn.1671-7414.2019.01.001]
　ZHANG Cui-ping,CHEN Ting-ting,WANG Cheng,et al.Levels and Its Significance of Urinary Exosomal miR-23b-3p in Children with Nephrotic Syndrome[J].Journal of Modern Laboratory Medicine,2019,34(01):1-4,9.[doi:10.3969/j.issn.1671-7414.2019.01.001]
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儿童原发性肾病综合征患者尿液外泌体miR-23b-3p水平变化及其临床意义()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第34卷
期数:
2019年01期
页码:
1-4,9
栏目:
论著
出版日期:
2019-02-28

文章信息/Info

Title:
Levels and Its Significance of Urinary Exosomal miR-23b-3p in Children with Nephrotic Syndrome
文章编号:
1671-7414 (2019)01-001-05
作者:
张翠平1; 陈婷婷1; 王成1; 于汉卿2; 张辰 宇3; 张春妮1
(1.南京大学医学院附属金陵医院，解放军东部战区总医院临床检验科， 南京210002； 2.南京医科大学附属儿童医院，南京210008；3.南京大学生命科学学院，南京 210046)
Author(s):
ZHANG Cui-ping1; CHEN Ting-ting1; WANG Cheng1;  YU Han-qing2;  ZHANG Chen-Yu3; ZHANG Chun-ni1
(1.Department of Clinical Laboratory， Jinling Hospital，Medical School of Nanjing University，Eastern Theater General Hospital of PLA，Nanjing 210002，China；2.Children’s Hospital of Nanjing Medical University， Nanjing 210008，China；3.School of Life Sciences，Nanjing University，Nan jing 210046，China)
关键词:
儿童原发性肾病综合征; 尿液; 外泌体; miR-23b-3p
分类号:
R692；R392.11
DOI:
10.3969/j.issn.1671-7414.2019.01.001
文献标志码:
A
摘要:
目的检测尿液外泌体miR-23b-3p在儿童原发性肾病综合征(nephrotic syndrome，NS)患者中的水平，评估其作为NS辅助诊断指标的临床价值。 方法收集2017年3~11月在解放军东部战区总医院住院并确诊的98例NS患儿(NS组)和南京医科大学附属儿童医院95例年龄、性别相匹配的健康儿童(对照组)的晨尿标 本及临床资料。实时荧光定量PCR (quantitative real-time PCR，qRT-PCR)方法检测miR -23b-3p在尿液外泌体中的含量。应用受试者工作特征曲线(receiver operating charact eristic curve，ROC)、相关性分析等评估其作为儿童NS诊断指标的临床价值。 结果miR-23b-3p在NS组尿液外泌体中的浓度明显高于对照组［13.44(4.47，31.40)fmol/L vs 3.94(2.76，7.15)fmol/L，差异有统计学意义， U=1 899，P <0 .000 1］，且在治疗缓解后显著下降［10.62(4.38，37.72)fmol/L vs 2.84(1.53，4 .92)fmol/L， W=695，P <0.000 1］。miR-23b-3p诊断NS的ROC曲线下面积(AUC)为0.796(95%CI：0.734～0.858)，敏感度为84.7%，特异度为64.2%。相关性分析显示，NS患儿尿液外泌体miR-23b-3p与血清总蛋白( r=－0.377)、清蛋白(r =－0.309)水平呈显著负相关，与血清总胆固醇( r=0.266)、三酰甘油(r =0.318)呈显著正相关，差异均具有统计学意义(均 P <0.01)。结论miR-23b-3p在NS患儿尿液外泌体中的含量显著升高，且在治疗缓解后明显降低，有望作为儿童NS 辅助诊断和治疗效果监测的潜在指标。
Abstract:
Objective To investigate the levels of urinary exosomal miR-23b-3p in children with nephrotic syndrome (NS) and evaluate its clinical significance as a non-invasive auxiliary diagnostic indicator．Methods Urinary samples were collected from 98 children with NS and 95 age-and gender-matched healthy controls in Eastern Theater General Hosp ital of PLA and Children’s Hospital of Nanjing Medical University from March to November 2017，respectively．The concentrations of urinary exosomal miR-23b-3 p were measured by quantitative real-time polymerase chain reaction (qRT-PCR) in NS and control children．The receiver operating characteristic (ROC) curves a nd correlation analysis were conducted to evaluate the diagnostic value of urina ry exosomal miR-23b-3p for children with NS．Results Ur inary exosomal miR-23b-3p was significantly evaluated in NS group as compared with control group ［13.44 (4.47，31.40) fmol/L vs 3.94 (2.76，7.15) fmol/ L］，the difference was statistically significant ( U=1 899，P <0.000 1)，and markedly declined in clinical remission period ［10.62 (4.38，37.72) fmol/L vs 2.84 (1.53，4.92) fmol/L， W=695，P <0.000 1］．The ROC analysis indica ted that the area under the curve (AUC) was 0.796 (95%CI：0.734~0.858) for th e diagnosis of NS children，with sensitivity of 84.7% and specificity of 64.2% ．Spearman correlations analysis indicated that the concentration of the miR-23 b-3p was significantly negatively correlated with serum total protein ( r =－ 0.377) and serum albumin ( r =－0.309)，and was significantly positively cor related with serum total cholesterol ( r =0.266) and triacylglycerols ( r = 0.318) in NS children，all with statistical significance (all P <0.01)．Conclusion The concentration of miR-23b-3p in urine exosom es was significantly elevated in children with NS，and was markedly reduced afte r treatment remission，suggesting that urinary exosomal miR-23b-3p may have th e potential to serve as an auxiliary diagnostic indicator for NS．

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备注/Memo

备注/Memo:
基金项目：国家自然科学基金 (81472021，81672102)。
作者简介：张翠平(1992-)，女，医学硕士研究生，主要研究方向 ：临床检验诊断学，E-mail：zhangcuiping1992@163.com。
通讯作者：张春妮(1963-)， 女，博士，主任技师，博士生导师 ，主要研究方向：临床检验诊断学，E-mail： zchunni27@hotmail.com。
更新日期/Last Update: 2019-02-28