[1]师亚玲,丰　航,廉江平,等.酶放大免疫法与荧光免疫层析法检测万古霉素血液浓度的对比研究[J].现代检验医学杂志,2022,37(01):168-171.[doi:10.3969/j.issn.1671-7414.2022.01.034]
　SHI Ya-ling,FENG Hang,LIAN Jiang-ping,et al.Comparison of Enzyme-multiplied Immunoassay and Fluorescence Immunochromatography in the Detecting Blood Concentrations of Vancomycin[J].Journal of Modern Laboratory Medicine,2022,37(01):168-171.[doi:10.3969/j.issn.1671-7414.2022.01.034]
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酶放大免疫法与荧光免疫层析法检测万古霉素血液浓度的对比研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第37卷
期数:
2022年01期
页码:
168-171
栏目:
研究简报 实验技术
出版日期:
2022-01-15

文章信息/Info

Title:
Comparison of Enzyme-multiplied Immunoassay and Fluorescence Immunochromatography in the Detecting Blood Concentrations of Vancomycin
文章编号:
1671-7414（2022）01-168-04
作者:
师亚玲; 丰　航; 廉江平; 张　鹏; 李　黎
（陕西省人民医院药学部，西安 710068）
Author(s):
SHI Ya-ling;  FENG Hang;  LIAN Jiang-ping;  ZHANG Peng;  LI Li
（Department of Pharmacy, Shaanxi Provincial People’s Hospital, Xi’an 710068, China）
关键词:
酶放大免疫法; 荧光免疫层析法; 万古霉素; 血液浓度
分类号:
Q503；R978.1
DOI:
10.3969/j.issn.1671-7414.2022.01.034
文献标志码:
A
摘要:
目的　比较酶放大免疫法（enzyme-multiplied immunoassay technique，EMIT）和荧光免疫层析法（.uorescence immunochromatography assay, FICA）测定万古霉素血液浓度的结果及相关性分析。方法　收集 2020年 9月～ 11月需要进行万古霉素血液浓度检测的样本 121例，分别采用 EMIT法和 FICA法测定万古霉素血液浓度，使用 SPSS18.0进行配对 t检验及 Pearson相关性分析，采用 Bland-Altman分析测定结果的一致性。结果　EMIT法测定回收率在
Abstract:
Objective　To compare the results of enzyme-multiplied immunoassay technique (EMIT) and fluorescenceimmunochromatography assay(FICA) for quantitative determination of vancomycin concentrations in serum. Method　121blood samples were collected from September 2020 to November 2020 for determination of vancomycin concentrations by theEMIT and FICA respectively. SPSS18.0 was used for t test and correlation analysis, and Bland-Altman plot was used for theconsistency analysis. Results　The recoveries of EMIT method and FICA were 99.8%~106.4% and 98.4%~107.2% respectively.The precision of the two methods were less than 20%, and both of them met the requirements of biological sample verification.The mean value of 121 samples determined by EMIT method was 18.83±11.24μg/ml, and the mean value of FICA was18.16±9.67μg/ml. The results of paired t test showed that there was no statistical difference between the two methods (t=1.584,P=0.117). The correlation coefficient of Pearson analysis was r=0.946 (P < 0.05), and 115 data of Bland-Altman plot were withinthe consistency limit (95.0%), indicating that the two detection methods had good correlation and consistency. Conclusion　Blood concentration of vancomycin determined by EMIT method was higher than that by FICA. EMIT and FICA assay are botheffective and reliable methods for vancomycin concentration monitoring. The samples, laboratory conditions, personnel allocationand other factors should be taken into account when selecting the determination method in clinical practice.

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备注/Memo

备注/Memo:
作者简介：师亚玲（1991-），女，硕士，主管药师，专业：临床药学，E-mail:xjshiyaling@163.com。
通讯作者：李黎（1986-），女，硕士，主管药师，专业：临床药学，E-mail:18829573334@163.com。
更新日期/Last Update: 1900-01-01