[1]汪佳婕,潘永圣,安 婧,等.云南地区肾移植受者BK 病毒感染与HLA 等位基因相关性分析[J].现代检验医学杂志,2022,37(05):14-17.[doi:10.3969/j.issn.1671-7414.2022.05.004]
　WANG Jia-jie,PAN Yong-sheng,AN Jing,et al.Correlation Analysis between BK Virus Infection and HLA Alleles in Renal Transplant Recipients in Yunnan[J].Journal of Modern Laboratory Medicine,2022,37(05):14-17.[doi:10.3969/j.issn.1671-7414.2022.05.004]
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云南地区肾移植受者BK 病毒感染与HLA 等位基因相关性分析()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第37卷
期数:
2022年05期
页码:
14-17
栏目:
论著
出版日期:
2022-09-15

文章信息/Info

Title:
Correlation Analysis between BK Virus Infection and HLA Alleles in Renal Transplant Recipients in Yunnan
文章编号:
1671-7414（2022）05-014-04
作者:
汪佳婕; 潘永圣 ; 安 婧; 蒲 丹
昆明市第一人民医院检验科，昆明 650011
Author(s):
WANG Jia-jie;  PAN Yong-sheng;  AN Jing;  PU Dan
Department of Laboratory Medicine, the First People’s Hospital of Kunming City, Kunming 650011，China
关键词:
BK 病毒感染; 人类白细胞抗原（HLA）; 肾移植受者
分类号:
R373；Q786
DOI:
10.3969/j.issn.1671-7414.2022.05.004
文献标志码:
A
摘要:
目的　研究云南地区肾移植受者BK 病毒（BK virus，BKV）感染与人类白细胞抗原（human leukocyteantigen，HLA）等位基因HLA-B,HLA-DR 抗原分布频率，探讨肾移植受者BKV 感染与HLA-B，HLA-DR 抗原之间的相关性。方法　选取昆明市第一人民医院自2017 年6 月～ 2020 年12 月肾移植受者399 例为研究对象，按照BKV感染与否分为BKV 感染组(n=126) 和BKV 未感染组(n=273)。应用聚合酶链反应序列特异性引物技术（polymerasechain reaction sequence specific primer，PCR-SSP）检测HLA-B 和HLA-DR 等位基因，用SPSS 软件分析肾移植受者BKV 感染尿液阳性HLA-B 和HLA-DR 抗原分布频率，比较HLA-B 和HLA-DR 抗原与BKV 感染不同结局的关系。结果　BKV 未感染组检出的HLA-B 等位基因有33 种，占比大于10% 的基因型有7 种，分别为 HLA-B*13（16.85%），HLA-B*46（19.41%），HLA-B*51（10.62%），HLA-B*55（10.62%），HLA-B*60（17.58%），HLA-B*62（24.91%）和HLA-B*75（16.48%）；BKV 感染组中检出的 HLA-B 等位基因有25 种，其中占比大于10% 的基因型有5 种，分别为HLA-B*46（24.60%），HLA-B*55（10.32%），HLA-B*60（24.60%），HLA-B*62（22.22%）和HLA-B*75（15.87%）。BKV 未感染组检出的HLA-DR 等位基因有14 种，其中占比大于10% 的基因型有7 种，分别为 HLA-DR*4（30.40%），HLA-DR*8（15.75%），HLA-DR*9（20.15%）， HLA-DR*11（12.45%），HLA-DR*12（42.12%）, HLA-DR*14（20.15%）和HLA-DR*15（25.27%）；BKV 感染组中检出的 HLA-DR 等位基因有14 种，其中占比大于5% 的基因型有8 种，分别为 HLA-DR*4（27.78%）， HLA-DR*8（18.25%）， HLA-DR*9（16.67%）， HLA-DR*11（11.11%），HLA-DR*12（42.86%），HLA-DR*13（11.90%），HLA-DR*14（23.81%）和HLA-DR*15（19.84%）。HLA-B*13 等位基因在BKV 未感染组检出率高于BKV感染组（χ2=4.642，P=0.031）；HLA-DR*17 等位基因在BKV感染组的检出率高于BKV未感染组（χ2=4.791，P=0.029）。结论　云南地区肾移植受者 HLA-B*13 等位基因可能是抗BKV 的保护因子；而HLA-DR*17 等位基因可能与BKV 感染的机会呈正相关。
Abstract:
Objective　To study the frequency of BK virus (BKV) infection of human leukocyte antigen (HLA)-B and HLA-DR antigen in kidney transplant recipients in Yunnan，and explore the correlation between BKV infection and HLA-B,HLA-DR antigen in kidney transplant recipients. Methods　A total of 399 kidney transplant recipients aged 3 to 70 years from June 2017 to December 2020 in the First People’s Hospital of Kunming City were selected as the research. They were divided into BKV infected group (126 cases) and BKV uninfected group (273 cases) according to whether they were infection or not. Used polymerase chain reaction sequence-specific primer technology (PCR-SSP) to detect HLA-B, HLA-DR alleles, and use SPSS software to analyze the distribution frequency of BKV-positive urine HLA-B and HLA-DR antigens in kidney transplant recipients, and compare the relationship between HLA-B and HLA-DR antigens and different outcomes of BKV infection. Results　There were 33 HLA-B alleles detected in BKV unaffected group, and 7 genotypes that accounted for more than 10% were HLA-B*13（16.85%)，HLA-B*46（19.41%）,HLA-B*51（10.62%）,HLA-B*55（10.62%）,HLA-B*60（17.58%）,HLA-B*62 （24.91%）and HLA-B*75（16.48%）.There were 25 HLA-B alleles detected in the BKV infection group, among which 5 genotypes account for more than 10%, namely HLA-B*46（24.60%）,HLA-B*55（10.32%）,HLA-B*60（24.60%）, HLA-B*62（22.22%）and HLA-B*75（15.87%）.There were 14 HLA-DR alleles detected in BKV unaffected group，of which 7 genotypes accounted for more than 10%, namely HLA-DR*4（30.40%）,HLA-DR*8（15.75%）, HLADR* 9（20.15%）, HLA-DR*11（12.45%）, HLA-DR*12（42.12%）, HLA-DR*14（20.15%）and HLA-DR*15（25.27%）. There were 14 HLA-DR alleles detected in the BKV infection group, of which 8 genotypes account for more than 10%, namely HLA-DR*4（27.78%）, HLA-DR*8（18.25%）, HLA-DR*9（16.67%）, HLA-DR*11（11.11%）,HLA-DR*12（42.86%）， HLA-DR*13（11.90%），HLA-DR*14（23.81%），HLA-DR*15（19.84%）. HLA-B*13 allele in BKV unaffected group detection rate is higher than BKV infection group(χ2=4.642，P=0.031). HLA-DR*17 Alleles in the BKV infection group was higher than the BK virus unaffected group(χ2=4.791，P=0.029). Conclusion　HLA-B*13 allele in the Yunnan area may be a protective factor of anti-BK viruses，and the chance of BK virus infection was positively correlated with HLA- DR*17.

参考文献/References:

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相似文献/References:

[1]杨小柯,何柳媚,全湛柔,等.深圳地区人群乙型肝炎病毒携带者HLA-DRB1 等位基因多态性及关联性分析[J].现代检验医学杂志,2020,35(05):5.[doi:10.3969/j.issn.1671-7414.2020.05.002]
　YANG Xiao-ke,HE Liu-mei,QUAN Zhan-rou,et al.Analysis of Allelic Polymorphism and Association of HLA-DRB1 in Hepatitis B Virus Carriers in Shenzhen[J].Journal of Modern Laboratory Medicine,2020,35(05):5.[doi:10.3969/j.issn.1671-7414.2020.05.002]

备注/Memo

备注/Memo:
作者简介：汪佳婕（1989-），女，硕士，初级技师，研究方向：免疫学及分子生物学检验，E-mail: 467315416@qq.com。
更新日期/Last Update: 2022-09-15