[1]颜新生a,张 丹a,王 栋a,等.疑似肺炎患者BALF 样本应用tNGS 技术进行病原学诊断的价值研究[J].现代检验医学杂志,2023,38(05):12-16.[doi:10.3969/j.issn.1671-7414.2023.05.003]
　YAN Xinshenga,ZHANG Dana,WANG Donga,et al.Value of tNGS in the Etiological Diagnosis of BALF Samples from Suspected Pneumonia Patients[J].Journal of Modern Laboratory Medicine,2023,38(05):12-16.[doi:10.3969/j.issn.1671-7414.2023.05.003]
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疑似肺炎患者BALF 样本应用tNGS 技术进行病原学诊断的价值研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第38卷
期数:
2023年05期
页码:
12-16
栏目:
论著
出版日期:
2023-09-15

文章信息/Info

Title:
Value of tNGS in the Etiological Diagnosis of BALF Samples from Suspected Pneumonia Patients
文章编号:
1671-7414（2023）05-012-05
作者:
颜新生a; 张 丹a; 王 栋a; 张李涛a; 张真路a; 连姝文b
（武汉亚心总医院 a. 检验科；b. 药学部，武汉 430056）
Author(s):
YAN Xinshenga;  ZHANG Dana;  WANG Donga;  ZHANG Litaoa;  ZHANG Zhenlua;  LIAN Shuwenb
（a.Department of Clinical Laboratory; b.Department of Pharmacy, Wuhan Asia General Hospital, Wuhan 430056, China）
关键词:
靶向高通量测序; 支气管肺泡灌洗液; 病原体检测
分类号:
R563.1；R446.5
DOI:
10.3969/j.issn.1671-7414.2023.05.003
文献标志码:
A
摘要:
目的 探究靶向高通量测序（targeted next-generation sequencing，tNGS）技术在疑似肺炎患者支气管肺泡灌洗液（bronchoalveolar lavage fluid，BALF）样本中的病原学诊断价值。方法 选取2022 年7 月～ 2023 年3 月武汉亚心总医院收治的102 例疑似肺炎患者的102 份BALF 样本，对其同时进行了tNGS 检测和传统微生物学检测，并收集患者的一般临床资料。对纳入研究的102 例患者的tNGS 检测结果、微生物培养结果以及其他实验室检查结果进行回顾性分析。结果 tNGS 法的总体阳性检出率（93.14%）高于传统方法（58.82%），差异有统计学意义（χ2=32.903，P＜ 0.001）；tNGS 法在细菌、病毒以及混合感染方面的检出率（65.69%，55.89%，69.61%）高于传统方法（21.57%，6.86%，11.76%），差异有统计学意义（χ2=32.903，56.920，70.708，均P ＜ 0.001）。结论 tNGS 技术在疑似肺炎患者BALF样本中获得了较高的病原体检出率，可以有效应用于呼吸道感染性疾病的病原学诊断。
Abstract:
Objective To explore the value of targeted next-generation sequencing (tNGS) in etiological diagnosis of bronchoalveolar lavage fluid (BALF) samples from patients with suspected pneumonia. Methods 102 samples of BALF samples from 102 suspected pneumonia patients admitted to Wuhan Asia General Hospital from July 2022 to March 2023 were selected. tNGS and traditional microbiology tests were performed on them at the same time, and general clinical data of the patients were collected. tNGS test results, microbial culture results and other laboratory results of 102 patients were retrospectively analyzed. Results The overall positive detection rate of tNGS method (93.14%) was higher than that of traditional method (58.82%), and the difference was statistically significant (χ2=32.903, P< 0.001). The detection rates of bacterial, viral and mixed infections by tNGS method (65.69%，55.89%， 69.61%) were higher than those by traditional methods (21.57%，6.86%，11.76%), and the differences were statistically significant (χ2=32.903，56.920，70.708，all P< 0.001). Conclusion tNGS technique has a high pathogen detection rate in BALF samples of suspected pneumonia patients, and can be effectively used in the etiological diagnosis of respiratory infectious diseases.

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备注/Memo

备注/Memo:
基金项目：武汉市卫生健康委员会青年项目（WX21Q29）：利伐沙班血药浓度与高龄非瓣膜性房颤患者不良预后的关系研究。
作者简介：颜新生（1986-），男，硕士，副主任技师，研究方向：分子诊断学，E-mail：yanxinsheng@126.com。
通讯作者：连姝文（1985-），女，本科，主管药师，研究方向：合理用药，E-mail：lianshuwen@126.com。
更新日期/Last Update: 2023-09-15