[1]关李稳,刘真义,周真真,等.溃疡性结肠炎患者血清LncRNA TUG1 和miR-142-5p 表达与疾病活动度及预后的关系[J].现代检验医学杂志,2023,38(05):70-74+79.[doi:10.3969/j.issn.1671-7414.2023.05.013]
　GUAN Liwen,LIU Zhenyi,ZHOU Zhenzhen,et al.Relationship between Serum LncRNA TUG1 and miR-142-5p Expression and Disease Activity and Prognosis in Patients with Ulcerative Colitis[J].Journal of Modern Laboratory Medicine,2023,38(05):70-74+79.[doi:10.3969/j.issn.1671-7414.2023.05.013]
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溃疡性结肠炎患者血清LncRNA TUG1 和miR-142-5p 表达与疾病活动度及预后的关系()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第38卷
期数:
2023年05期
页码:
70-74+79
栏目:
论著
出版日期:
2023-09-15

文章信息/Info

Title:
Relationship between Serum LncRNA TUG1 and miR-142-5p Expression and Disease Activity and Prognosis in Patients with Ulcerative Colitis
文章编号:
1671-7414（2023）05-070-06
作者:
关李稳; 刘真义; 周真真; 王力雅; 阚 娜
（海南省第三人民医院消化内科，海南三亚 572000）
Author(s):
GUAN Liwen;  LIU Zhenyi;  ZHOU Zhenzhen;  WANG Liya;  KAN Na
（Department of Gastroenterology, the Third People’s Hospital of Hainan, Hainan Sanya 572000，China）
关键词:
溃疡性结肠炎; 长链非编码核糖核酸牛磺酸上调基因1; 微小核糖核酸-142-5p
分类号:
R574.62；R392.11
DOI:
10.3969/j.issn.1671-7414.2023.05.013
文献标志码:
A
摘要:
目的 分析溃疡性结肠炎（ulcerative colitis，UC）患者血清长链非编码核糖核酸牛磺酸上调基因1（long noncodingRNA taurine upregulated gene 1，LncRNA TUG1）、微小核糖核酸（microRNA，miR）-142-5p 表达水平与UC 疾病活动度及预后的关系。方法 选取2017 年1 月～ 2020 年5 月期间海南省第三人民医院收治的UC 患者108 例为UC组，根据改良Mayo 评分将UC 患者分为缓解期组（Mayo 评分在2 分及以下而且无单个分项评分在1 分，n=42）、轻度活动组（3 分≤ Mayo 评分≤ 5 分，n=21）、中度活动组（6 分≤ Mayo 评分≤ 10 分，n=24）和重度活动组（11 分≤ Mayo 评分≤ 12 分，n=21）。对UC 患者随访2 年，记录其复发情况。另选取同期体检健康者108 例为健康组。实时荧光定量PCR 法测定血清LncRNA TUG1 和miR-142-5p 表达水平；比较UC 组和健康组、不同疾病活动度UC 患者、不同预后UC 患者血清LncRNA TUG1 和miR-142-5p 表达水平；分析UC 患者血清LncRNA TUG1 表达水平与miR-142-5p 的相关性及影响UC 患者预后的因素。结果 与健康组相比，UC 组患者血清LncRNA TUG1 表达水平（0.45±0.15 vs 1.04±0.35）降低，miR-142-5p 表达水平（1.76±0.32 vs 1.01±0.18）升高，差异均有统计学意义（t=16.102，21.229，均P ＜ 0.05）。UC 患者血清LncRNA TUG1 表达水平与miR-142-5p 呈负相关（r=-0.558，P ＜ 0.05）；缓解期组、轻度活动组、中度活动组和重度活动组血清LncRNA TUG1 表达水平依次降低（0.68±0.23，0.48±0.16，0.29±0.09，0.13±0.05），miR-142-5p 表达水平依次升高（1.48±0.27，1.55±0.28，1.97±0.36，2.30±0.42），差异有统计学意义（F=59.475，35.716，均P ＜ 0.05）。108 例UC 患者随访2 年，复发51 例（复发组），未复发57 例（未复发组），与未复发组相比，复发组UC 患者血清LncRNA TUG1 表达水平（0.38±0.13 vs 0.51±0.17）降低，miR-142-5p 表达水平（1.93±0.35 vs 1.61±0.29）和有肠外表现患者占比（21/30 vs 9/48）升高，差异均有统计学意义（t=4.424~8.647，均P ＜0.05）；肠外表现（OR=2.366，95%CI=1.558～ 3.592，P＜ 0.001）和miR-142-5p（OR=2.563，95%CI=1.649～ 3.984，P＜ 0.001）是影响UC 患者预后的危险因素，而LncRNA TUG1（OR=0.680，95%CI=0.557 ～ 0.830，P ＜ 0.001）是影响UC 患者预后的保护因素。结论 UC 患者血清LncRNA TUG1 呈低表达而miR-142-5p 呈高表达，均与UC 疾病活动度、预后密切相关，测定二者水平有助于临床评估UC 患者病情及预后。
Abstract:
108 UC patients accepted by the Third People’s Hospital of Hainan Province during January 2017 to May 2020 were gathered as the UC group. According to the modified Mayo score, patients with UC were divided into remission group (Mayo score was 2 or less and no single sub-score was more than 1, n=42) , mild activity group (3 ≤ Mayo score ≤ 5, n=21), moderate activity group (6 ≤ Mayo score ≤ 10, n=24) and mild activity group (11 ≤ Mayo score ≤ 12, n=21). The patients with UC were followed up for 2 years and the recurrence was recorded. In addition, 108 healthy people during physical examination were selected as the healthy group. Real-time fluorescent quantitative PCR method was used to determine the expression levels of serum LncRNA TUG1 and miR-142-5p. The expression levels of LncRNA TUG1 and miR-142-5p in serum were compared between UC group and healthy group, UC patients with different disease activity, and UC patients with different prognosis. Analyzed the correlation between the expression level of serum LncRNA TUG1 and miR-142-5p in UC patients and the prognostic factors of UC patients. Results Compared with the healthy group, the expression level of serum LncRNA TUG1 (0.45± 0.15 vs 1.04±0.35) in UC group was decreased, and the expression level of miR-142-5p (1.76±0.32 vs 1.01±0.18) was increased, the differences were statistically significant (t=16.102, 21.229, all P<0.05). The expression level of serum LncRNA TUG1 in UC patients was negatively correlated with miR-142-5p (r=-0.558, P<0.05). The expression level of serum LncRNA TUG1 (0.68±0.23, 0.48±0.16, 0.29±0.09, 0.13±0.05) in remission group, mild activity group, moderate activity group and severe activity group were successively decreased, the expression level of miR-142-5p (1.48±0.27, 1.55±0.28, 1.97±0.36, 2.30±0.42) were successively increased, and the differences were statistically significant (F=59.475, 35.716, all P<0.05). 108 UC patients were followed up for 2 years, 51 patients had recurrence (recurrence group) and 57 patients did not relapse (nonrecurrence group). Compared with the non-recurrence group, the expression level of serum LncRNA TUG1 (0.38±0.13 vs 0.51±0.17) in UC patients in the recurrence group was decreased, the expression level of miR-142-5p (1.93±0.35 vs 1.61±0.29) and the proportion of patients with parenteral manifestations (21/30 vs 9/48) were increased, and the differences were statistically significant (t=4.424 ～ 8.647, all P<0.05). Parenteral manifestations (OR=2.366, 95%CI：1.558 ～ 3.592, P<0.001) and miR-142-5p (OR=2.563, 95%CI：1.649 ～ 3.984, P<0.001) were risk factors for the prognosis of UC patients, and LncRNA TUG1 (OR=0.680, 95%CI：0.557 ～ 0.830, P<0.001) was a protective factor for the prognosis of UC patients. Conclusion The low expression of LncRNA TUG1 and the high expression of miR-142-5p in the serum of UC patients, were closely related to the disease activity and prognosis of UC, the determination of the two levels is helpful for clinical evaluation of the disease and prognosis of UC patients.

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备注/Memo

备注/Memo:
基金项目：海南省卫生健康行业科研项目（编号2102320242A2001）：血清Nesfatin-1 在炎症性肠病中的水平检测及临床意义的研究。
作者简介：关李稳（1985-），男，本科，主治医师，研究方向：炎症性肠病诊治，E-mail：glwen1930@163.com。
通讯作者：阚娜（1982-），女，硕士，主任医师，研究方向：消化道疾病诊治，E-mail：109758857@qq.com。
更新日期/Last Update: 2023-09-15