[1]白雪飞a,魏 敏,王 琪a,等.子宫内膜癌组织CKS1B mRNA水平表达与临床病理学特征及预后的相关性研究[J].现代检验医学杂志,2024,39(06):23-28.[doi:10.3969/j.issn.1671-7414.2024.06.004]
　BAI Xuefeia,WEI Min,WANG Qia,et al.Relationship of CKS1B mRNA Expression in Endometrial Cancer Tissues with Its Clinicopathological Features and Prognosis[J].Journal of Modern Laboratory Medicine,2024,39(06):23-28.[doi:10.3969/j.issn.1671-7414.2024.06.004]
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子宫内膜癌组织CKS1B mRNA水平表达与临床病理学特征及预后的相关性研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第39卷
期数:
2024年06期
页码:
23-28
栏目:
论著
出版日期:
2024-11-15

文章信息/Info

Title:
Relationship of CKS1B mRNA Expression in Endometrial Cancer Tissues with Its Clinicopathological Features and Prognosis
文章编号:
1671-7414（2024）06-023-06
作者:
白雪飞1a; 魏 敏2; 王 琪1a; 贾志红1a; 戴银桥1b
（1．中山大学附属肿瘤医院甘肃医院/ 甘肃省肿瘤医院 a. 妇瘤科；b. 病理科，兰州 730050；2. 兰州大学第一医院妇科，兰州 730013）
Author(s):
BAI Xuefei1a;  WEI Min2;  WANG Qi1a;  JIA Zhihong1a;  DAI Yinqiao1b
（1a. Department of Gynecological Oncology; 1b. Department of Pathology, Sun Yat-sen University Cancer Center Gansu Hospital/Gansu Provincial Cancer Hospital, Lanzhou 730050, China; 2. Department of Gynecology, the First Hospital of Lanzhou University, Lanzhou 730013, China）
关键词:
细胞周期依赖蛋白酶调节亚基1B; 子宫内膜癌; 癌症基因组图谱; 临床病理特征
分类号:
R737.33；R730.43
DOI:
10.3969/j.issn.1671-7414.2024.06.004
文献标志码:
A
摘要:
目的 研究细胞周期依赖蛋白酶调节亚基1B（cyclin-dependent kinases regulatory subunit 1B，CKS1B）与子宫内膜癌(endometrial carcinoma，EC) 患者临床病理特征及预后的相关性。方法 下载癌症基因组图谱（the CancerGenome Atlas，TCGA）数据库和基因型- 组织表达（genotype-tissue expression，GTEx）数据库中CKS1B 的表达谱数据和临床数据，分析CKS1B 在子宫内膜癌中的表达及与临床病理特征和预后的关系。通过UALCAN 数据库验证CKS1B 蛋白表达水平；Logistics 回归分析CKS1B 表达与临床病理参数的关系；COX 回归分析和Kaplan-Meier 分析临床病理参数与子宫内膜癌预后的相关性；分析TCGA 数据库中CKS1B 的共表达基因并富集分析。实时荧光定量PCR（quantitative real-time PCR，qRT-PCR）检测CKS1B mRNA 在Ishikawa 和HEC-1-A 细胞系中的表达。蛋白质免疫印迹（Western Bolt，WB）检测CKS1B 蛋白在子宫内膜癌组织和癌旁组织的表达。结果 CKS1B mRNA 和蛋白在子宫内膜癌中明显高于正常子宫内膜，差异具有统计学意义(P<0.05)。CKS1B mRNA 表达水平与患者FIGO 分期（F=42.994）、组织学分级（F=70.350）、组织学类型（F=87.341）和年龄（F=40.097）密切相关（均P<0.05）。Kaplan-Meier 法显示CKS1B mRNA 高表达组患者总生存率更低（Log-rank χ2=1.175，P<0.01）。多因素COX 分析显示CKS1B 表达水平（HR=1.856，95%CI：1.154 ～ 2.985）、FIGO 分期（HR=3.065，95%CI：1.906 ～ 4.926）为子宫内膜癌预后的独立风险因素（P<0.05）。GO 分析显示CKS1B 主要参与细胞核分裂、染色体分离。KEGG 分析显示主要在细胞周期、剪接体及DNA 复制通路富集。进一步验证与人子宫内膜上皮细胞比较CKS1B mRNA 在Ishikawa 和HEC-1-A 细胞中高表达（F=44.560，P<0.001），CKS1B 蛋白在子宫内膜癌组织中高表达（t=14.900，P<0.001）。结论 CKS1B 在子宫内膜癌中表达上调且与患者的临床病理因素及预后密切相关，可能通过细胞周期调控参与子宫内膜癌发生、发展，有望成为子宫内膜癌诊断和预测预后的新指标。
Abstract:
Objective To investigate the expression of CKS1B in endometrial carcinoma (EC) and its relationship with clinicopathological features and prognosis. Methods The expression profile data and clinical data of CKS1B from the TCGA and GTEx databases were downloaded to investigate the expression of CKS1B in EC and its relationship with clinicopathological features. The expression of CKS1B at the protein level was verified using the UALCAN database. The relationship between CKS1B expression and clinicopathological parameters was analyzed by Logistic regression. The r program perform enrichment analysis on CKS1B co-expressed genes in the TCGA database. Finally，CKS1B mRNA expression was discovered in the cell lines Ishikawa and HEC-1-A by quantitative real-time PCR(qRT-PCR).CKS1B protein expression was detected in EC tissues and adjacent tissues by Western Bolt(WB). Results CKS1B mRNA and protein were remarkably higher in EC tissues than in normal endometrium，and the differences were statistically significant (P<0.05). The level of CKS1B mRNA expression was strongly correlated with FIGO stage(F=42.994), histological grade(F=70.350), histological type(F=87.341) and age(F=40.097) (all P<0.05).The results of the Kaplan-Meier method showed that patients with high CKS1B mRNA expression had a lower overall survival rate(Log-rank χ2 =1.175，P<0.01). Multifactorial COX analysis showed that FIGO stage(HR=3.065， 95%CI：1.906 ～ 4.926)and CKS1B expression(HR=1.856，95%CI：1.154 ～ 2.985)were independent risk factors affecting the prognosis of patients with EC (P<0.05). GO analysis showed that CKS1B was mainly involved in nuclear division and chromosome separation. KEGG analysis showed it was mainly enriched in the cell cycle，spliceosome and DNA replication. Further verification showed that CKS1B mRNA was highly expressed in Ishikawa and HEC-1-A cell lines(F=44.560， P<0.001),CKS1B protein was highly expressed in EC tissues (t=14.900，P<0.001).Conclusion CKS1B is upregulated in EC and is linked to clinicopathological variables in the patients. It may play a role in the development of EC by regulating the cell cycle, and it is expected to be a new marker for the diagnosis and prognosis of EC.

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备注/Memo

备注/Memo:
基金项目：兰州市科技局科技计划项目（2023-2-13）；甘肃省科技厅自然科学基金（23JRRA1591）。
作者简介：白雪飞（1981-），女，硕士，主治医师，研究方向：妇科肿瘤， E-mail：baixuefei225722@163.com。
通讯作者：魏敏（1984-），女，硕士，副主任医师，研究方向：妇科肿瘤， E-mail: ldyy_weimldyy@lzu.edu.cn。
更新日期/Last Update: 2024-11-15