[1]杨小柯,何柳媚,全湛柔,等.深圳地区人群乙型肝炎病毒携带者HLA-DRB1 等位基因多态性及关联性分析[J].现代检验医学杂志,2020,35(05):5-8,32.[doi:10.3969/j.issn.1671-7414.2020.05.002]
　YANG Xiao-ke,HE Liu-mei,QUAN Zhan-rou,et al.Analysis of Allelic Polymorphism and Association of HLA-DRB1 in Hepatitis B Virus Carriers in Shenzhen[J].Journal of Modern Laboratory Medicine,2020,35(05):5-8,32.[doi:10.3969/j.issn.1671-7414.2020.05.002]
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深圳地区人群乙型肝炎病毒携带者HLA-DRB1 等位基因多态性及关联性分析()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第35卷
期数:
2020年05期
页码:
5-8,32
栏目:
论著
出版日期:
2020-11-01

文章信息/Info

Title:
Analysis of Allelic Polymorphism and Association of HLA-DRB1 in Hepatitis B Virus Carriers in Shenzhen
文章编号:
1671-7414（2020）05-005-05
作者:
杨小柯1; 何柳媚2; 全湛柔2; 高素青2
1. 深圳市疾病预防控制中心，广东深圳 518000；2. 深圳市血液中心输血医学研究所, 广东深圳 518000
Author(s):
YANG Xiao-ke1;  HE Liu-mei2;  QUAN Zhan-rou2;  GAO Su-qing2
1.Shenzhen Center for Disease Control and Prevention, Guangdong Shenzhen 518000,China; 2. Institute of Transfusion Medicine of Shenzhen Blood Center, Guangdong Shenzhen 518000,China
关键词:
乙型肝炎病毒携带者; 人类白细胞抗原（HLA）; 基因多态性
分类号:
R512.62；Q786
DOI:
10.3969/j.issn.1671-7414.2020.05.002
文献标志码:
A
摘要:
目的　研究深圳地区人群乙型肝炎病毒携带者人类白细胞抗原（human leukocyte antigen，HLA）DRB1 的等位 基因多态性分布特征，探讨乙型肝炎病毒携带与HLA-DRB1 等位基因之间的关联性。方法　采用聚合酶链反应- 直接 测序分型技术（PCR-SBT）对163 例深圳地区乙型肝炎病毒携带者（携带组）和314 例健康人群（对照组）进行HLADRB1 等位基因的检测，运用统计学方法比对分析两组人群的基因分型结果。结果　对照组检出的HLA-DRB1 等位基 因有32 种，其中占比大于5% 的基因型有6 种，分别为HLA-DRB1*09:01（16.40%），12:02（11.15%），15:01（9.87%）， 08:03（8.91%），11:01（6.05%）和04:05（5.10%）；携带组中检出的HLA-DRB1 等位基因有28 种，其中占比大于5% 的基因型有6 种，分别为HLA-DRB1*09:01（20.55%），12:02（14.72%），15:01（9.20%），03:01（7.06%）、08:03（5.52%） 和11:01（5.21%）。其中，携带组中HLA-DRB1*03:01 检出率高于对照组，差异有统计学意义（P=0.010）。结论　深 圳地区人群感染乙肝病毒的机会与HLA-DRB1*03:01 呈正相关。
Abstract:
Objective　To study the distribution characteristics of allelic polymorphism of human leukocyte antigen (HLA) DRB1 in Hepatitis B virus carriers in Shenzhen area, and explore the association between Hepatitis B virus and HLA-DRB1 allele. Methods　HLA-DRB1 alleles were detected in 163 Hepatitis B virus carriers (the carrying group) and 314 healthy people (the control group) in Shenzhen by polymerase chain react-direct sequencing typing (PCR-SBT). The genotyping results of the two groups were compared using statistical methods. Results　There were 32 HLA-DRB1 alleles detected in the control group, among which 6 genotypes with a proportion greater than 5% were HLA-DRB1*09:01 (16.40%), 12:02 (11.15%), 15:01 (9.87%), 08:03 (8.91%), 11:01 (6.05%) and 04:05 (5.10%)，respectively . And there were 28 alleles of HLA-DRB1 detected in the carrying group, including 6 genotypes with a proportion greater than 5%, which were HLA-DRB1*09:01 (20.55%), 12:02 (14.72%), 15:01 (9.20%), 03:01 (7.06%), 08:03 (5.52%)and 11:01 (5.21%)，respectively. Among them, the detection rate of HLA-DRB1*03:01 in the carrying group was higher than that in the control group, and the P value showed a significant difference (P=0.010). Conclusion　 The chance of Hepatitis B virus infection in Shenzhen area was positively correlated with HLA- DRB1*03:01.

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备注/Memo

备注/Memo:
基因项目：深圳市科技计划项目（JCYJ20160425100217087）。
作者简介：杨小柯（1980-），男，硕士研究生，副主任技师，研究方向：微生物检验，E-mail：15319544@qq.com。
通讯作者：高素青（1965-），女，本科，研究方向：免疫遗传，E-mail：1597743067@qq.com。
更新日期/Last Update: 2020-10-30