[1]陈建芸a,廖海平a,孙朝晖a,等.类风湿性关节炎患者血清RA-CP，抗CCP 抗体及RF 检测对RA 的实验诊断意义[J].现代检验医学杂志,2020,35(05):38-40,50.[doi:10.3969/j.issn.1671-7414.2020.05.010]
　CHEN Jian-yuna,LIAO Hai-pinga,SUN Zhao-huia,et al.Significance of the Detection of RA-CP, Anti-CCP Antibody and RF in the Laboratory Diagnosis of Rheumatoid Arthritis[J].Journal of Modern Laboratory Medicine,2020,35(05):38-40,50.[doi:10.3969/j.issn.1671-7414.2020.05.010]
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类风湿性关节炎患者血清RA-CP，抗CCP 抗体及RF 检测对RA 的实验诊断意义()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第35卷
期数:
2020年05期
页码:
38-40,50
栏目:
论著
出版日期:
2020-11-01

文章信息/Info

Title:
Significance of the Detection of RA-CP, Anti-CCP Antibody and RF in the Laboratory Diagnosis of Rheumatoid Arthritis
文章编号:
1671-7414（2020）05-038-04
作者:
陈建芸a; 廖海平a; 孙朝晖a; 李林海a; 张宏斌b
南部战区总医院a. 检验科；b. 医学实验科, 广州 510010
Author(s):
CHEN Jian-yuna; LIAO Hai-pinga; SUN Zhao-huia; LI Lin-haia; ZHANG Hong-binb
a.Department of Clinical Laboratory; b.Department of Medical Research, General Hospital of Southern Theater Command, Guangzhou 510010,China
关键词:
类风湿性关节炎特异性抗原瓜氨酸化蛋白; 类风湿性关节炎; 抗环瓜氨酸多肽抗体; 类风湿因子
分类号:
R593.22；R392.11
DOI:
10.3969/j.issn.1671-7414.2020.05.010
文献标志码:
A
摘要:
目的　分析不同血清类型的类风湿性关节炎（RA）患者血清中的类风湿性关节炎特异性抗原瓜氨酸化蛋白（RACP） 的水平，比较RA-CP 与抗环瓜氨酸多肽（CCP）抗体、类风湿因子（RF）在RA 诊断中的价值。方法　用酶联免疫 吸附法（ELISA）检测103 例RA 患者、71 例其他风湿病患者、105 例健康体检者血清中RA-CP，抗CCP 抗体和RF 的 水平。根据抗CCP 抗体和RF 测定结果将RA 患者分为4 种血清类型：抗CCP 抗体阳性/RF 阳性的RA、抗CCP 抗体 阳性/RF 阴性的RA、抗CCP 抗体阴性/RF 阳性的RA、抗CCP 抗体阴性/RF 阴性的RA，分析各种血清类型RA 患者 血清中RA-CP 的水平。结果　RA 组的血清中RA-CP 浓度水平中位数为4.51（1.80~7.49）单位，高于其他疾病组的0.53 （0.40~0.76）单位和健康对照组的0.52（0.35~0.69）单位。RA-CP，抗CCP 抗体和RF 对RA 的敏感度分别为77.7%， 65.0% 和69.9%，RA-CP 对RA 的敏感度高于抗CCP 抗体和RF（P ＜ 0.05）；特异度分别为95.5%，97.2% 和89.2%。在 4 种血清型的RA 中RA-CP 的检出率分别为100.0%（57/57），40.0%（4/10），100.0%（15/15）和19.0%（4/21）。在抗 CCP 抗体阴性的RA 中RA-CP 的检出率为52.8%（19/36）。结论　血清中RA-CP 对RA 的诊断比抗CCP 抗体和RF 具有 更高的敏感度，可提高类风湿性关节炎的检出率。因此RA-CP 可作为RA 临床诊断的新辅助指标。
Abstract:
Objective　To analyze the level of the rheumatoid arthritis-specific citrullinated protein (RA-CP) in patients of different serum types with rheumatoid arthritis (RA), and compare the value of RA-CP, anti-cyclic citrullinated polypeptide (CCP) and rheumatoid factor (RF) in the diagnosis of RA. Methods　Enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of RA-CP, anti-CCP antibodies and RF in the serum of 103 RA patients, 71 other rheumatism patients and 105 healthy person. According to the results of CCP and RF, RA patients were divided into 4 serotypes: anti-CCP antibody positive/ RF positive RA, anti-CCP antibody positive/RF negative RA, anti-CCP antibody negative/RF positive RA, anti-CCP antibodynegative/ RF-negative RA. The level of RA-CP in various serum types RA were test. Results　The median RA-CP concentration in serum of RA group was 4.51 (1.80 ~ 7.49) units, which was higher than 0.53 (0.40 ~ 0.76) units of other disease groups and 0.52 (0.35 ~ 0.69) units of healthy controls. The sensitivity of RA-CP, anti-CCP antibody and RF to RA was 77.7%, 65.0% and 69.9% respectively and the sensitivity of RA-CP was higher than anti CCP antibody and RF(P ＜ 0.05). The specificity was 95.5%, 97.2% and 89.2% respectively. For the four serotypes of RA, RA-CP detection rates were 100.0% (57/57), 40.0% (4/10), 100.0% (15/15) and 19.0% (4/21). Specially, in anti-CCP antibody negative RA group, the detection rate of RA-CP was 52.8% (19/36). Conclusion　RA-CP had higher sensitivity for the diagnosis of RA than anti-CCP antibody and RF, and RA-CP test could improve the detection rate of rheumatoid arthritis. Therefore, RA-CP can be used as a new target for clinical diagnosis of RA.

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备注/Memo

备注/Memo:
基金项目：广州市科技计划项目（201604020106）。
作者简介：陈建芸（1974-），女，副主任技师，研究方向：免疫检验及相关疾病，E-mail：380350681@qq.com。
通讯作者：张宏斌（1973-），男，博士，主任技师，硕士研究生导师，E-mail：zhangwater@tom.com。
更新日期/Last Update: 2020-10-30