[1]喻　晶,吴夏枫,刘晓翌,等.新型冠状病毒肺炎（COVID-19）热灭活咽拭子及热灭活后提取核酸样本在 4℃保存的稳定性研究[J].现代检验医学杂志,2020,35(06):132-134.[doi:doi:10.3969/j.issn.1671-7414.2020.06.032]
　YU Jing,WU Xia-feng,LIU Xiao-yi,et al.Study on the Stability of Heat-Inactivated Throat Swab and Its ExtractedNucleic Acid of COVID-19 Stored at 4℃[J].Journal of Modern Laboratory Medicine,2020,35(06):132-134.[doi:doi:10.3969/j.issn.1671-7414.2020.06.032]
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新型冠状病毒肺炎（COVID-19）热灭活咽拭子及热灭活后提取核酸样本在 4℃保存的稳定性研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第35卷
期数:
2020年06期
页码:
132-134
栏目:
新型冠状病毒肺炎检验专题
出版日期:
2020-12-30

文章信息/Info

Title:
Study on the Stability of Heat-Inactivated Throat Swab and Its ExtractedNucleic Acid of COVID-19 Stored at 4℃
文章编号:
1671-7414（2020）06-132-03
作者:
喻　晶; 吴夏枫; 刘晓翌; 纪　玲
（北京大学深圳医院检验科，广东深圳 518029）
Author(s):
YU Jing;  WU Xia-feng;  LIU Xiao-yi;  JI Ling
（Department of Laboratory Medicine, Peking University Shenzhen Hospital, Guangdong Shenzhen 518029, China）
关键词:
新型冠状病毒肺炎; 热灭活; 咽拭子样本; 提取核酸; 4℃保存
分类号:
R373.19；R446.5
DOI:
doi:10.3969/j.issn.1671-7414.2020.06.032
摘要:
目的　研究 56℃ 30 min 热灭活后的新型冠状病毒肺炎 (COVID-19) 咽拭子及提取的核酸在 4℃保存的稳定性。方法　采用多重荧光定量 PCR 方法对一例 COVID-19 患者的咽拭子样本，56℃ 30 min 热灭活后进行 COVID-19 双靶点(ORF1ab 和 N 基因 ) 核酸检测，把原始咽拭子样本及提取的核酸保存在 4℃，分别于 24，48，72 和 96h 再次检测咽拭子样本及提取核酸中新冠病毒核酸的含量（Ct 值），分析热灭活后咽拭子样本及提取核酸在 4℃保存的稳定性。结果COVID-19 患者的咽拭子样本，56℃ 30 min 热灭活后核酸检测结果为 ORF1ab 和 N 基因双阳性，Ct 值分别为 31.27 和30.64，咽拭子样本 4℃保存 24，48，72 和 96h 后再次检测 ORF1ab 和 N 基因的结果为 24h (31.41 和 30.68)，48h (34.13和 34.28)，72h (34.06 和 34.11) 和 96h (36.22 和 40.02)。 提取的核酸 4℃保存 24，48，72 和 96h 后的检测结果为 24h (31.64和 30.06)，48h (31.62 和 29.95)，72h (31.72 和 29.75) 和 96h (31.36 和 30.09)。56℃ 30 min 热灭活的咽拭子样本在 4℃保存 24h 后，对 COVID-19 核酸的检测无明显影响 ( 偏倚 <1%)，但 4℃保存 48~72h，ORF1ab 基因核酸降解约 7 倍，N 基因降解约 11 倍，4℃保存 96h, ORF1ab 和 N 基因核酸分别降解 30.91 倍和 666.29 倍。但是提取的核酸 4℃保存 24~96h 后，对核酸的检测结果无明显影响 ( 偏倚均小于 3%)。结论　研究结果表明 56℃ 30 min 热灭活后提取的核酸的稳定性比原始咽拭子样本好，热灭活后的咽拭子样本可在 4℃保存 24h。

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更新日期/Last Update: 2020-12-20