[1]李 娅,皇 海,张 赟,等.HSV-TK/GCV系统在体外对胃癌MNK-45细胞株的增殖抑制作用研究[J].现代检验医学杂志,2021,36(02):19-23.[doi:doi:10.3969/j.issn.1671-7414.2021.02.005]
 LI Ya,Huang Hai,ZHANG Yun,et al.Study on the Inhibition of HSV-TK/GCV System on the Proliferation of Gastric Cancer MNK-45 Cell Line in Vitro[J].Journal of Modern Laboratory Medicine,2021,36(02):19-23.[doi:doi:10.3969/j.issn.1671-7414.2021.02.005]
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HSV-TK/GCV系统在体外对胃癌MNK-45细胞株的增殖抑制作用研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第36卷
期数:
2021年02期
页码:
19-23
栏目:
论 著
出版日期:
2021-04-30

文章信息/Info

Title:
Study on the Inhibition of HSV-TK/GCV System on the Proliferation of Gastric Cancer MNK-45 Cell Line in Vitro
文章编号:
1671-7414(2021)02-019-06
作者:
李 娅皇 海张 赟
(1.西安市人民医院(西安市第四医院)检验科,西安 710004; 2.空军军医大学第一附属医院全军临床检验医学研究所,西安710032)
Author(s):
LI Ya Huang Hai ZHANG Yun et al
[1.Departments of Clinical Laboratory, Xi’an People’s Hospital(Xi’an Fourth Hospital), Xi’an 710004, China; 2. Institute of Clinical Laboratory Medicine of People’s Liberation Army, the First Affiliated Hospital of AFMU, Xi’an 710032, China]
关键词:
胃癌单纯疱疹病毒胸苷激酶基因/丙氧鸟苷系统MNK-45细胞细胞增殖
分类号:
R735.2;R730.43
DOI:
doi:10.3969/j.issn.1671-7414.2021.02.005
文献标志码:
A
摘要:
目的 探讨单纯疱疹病毒胸苷激酶基因/丙氧鸟苷(HSV-TK/GCV)自杀基因系统对胃癌MNK-45细胞增殖、侵袭和迁移的抑制效应。方法 分别采用MTS试验、CCK8试验、Transwell试验、Western blot试验和RT-PCR方法检测HSV-TK/GCV系统对胃癌MNK-45细胞在24,48,72和96h的增殖、侵袭和迁移的改变;对MNK-45细胞凋亡相关蛋白mRNA水平的变化。结果 HSV-TK/GCV系统能够有效抑制胃癌MNK-45细胞的增殖效应,并能够减弱胃癌MNK-45细胞的侵袭和迁移能力,诱导MNK-45细胞凋亡相关蛋白Caspase3、Bax蛋白水平和mRNA的表达增加,抑制Bcl-2蛋白表达水平的降低,促进凋亡的发生。结论 HSV-TK/GCV系统能够有效抑制胃癌MNK-45细胞增殖、侵袭和迁移,诱导MNK-45细胞凋亡的发生。

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:陕西省科技厅一般项目-社会发展领域(2017SF-265),西安市人民医院(西安市第四医院)孵化基金CX-20。
作者简介:李娅(1980-)女,副主任检验师,研究方向:肿瘤分子机制研究,E-mail:38647846@qq.com。
通讯作者:屈慧,主管技师,E-mail:quhui1113@163.com。
更新日期/Last Update: 2021-04-10