[1]李 品a,刘春晖a,石军荣a,等.卵巢癌患者血清微小RNA 128 的表达水平及与化疗效果和预后的相关性研究[J].现代检验医学杂志,2022,37(06):104-109.[doi:10.3969/j.issn.1671-7414.2022.06.019]
 LI Pina,LIU Chun-huia,SHI Jun-ronga,et al.Expression Level of Serum miRNA-128 in Patients with Ovarian Cancer and Its Correlation with Chemotherapy Effect and Prognosis[J].Journal of Modern Laboratory Medicine,2022,37(06):104-109.[doi:10.3969/j.issn.1671-7414.2022.06.019]
点击复制

卵巢癌患者血清微小RNA 128 的表达水平及与化疗效果和预后的相关性研究()
分享到:

《现代检验医学杂志》[ISSN:/CN:]

卷:
第37卷
期数:
2022年06期
页码:
104-109
栏目:
论著
出版日期:
2022-11-15

文章信息/Info

Title:
Expression Level of Serum miRNA-128 in Patients with Ovarian Cancer and Its Correlation with Chemotherapy Effect and Prognosis
文章编号:
1671-7414(2022)06-104-06
作者:
李 品a刘春晖a石军荣a蔡智慧a侯 岩b李晶晶a齐 冀a闫丽伟a
(河北大学附属医院 a. 妇科;b.CT/MRI 诊断,河北保定 071000)
Author(s):
LI Pina LIU Chun-huia SHI Jun-ronga CAI Zhi-huia HOU Yanb LI Jing-jinga QI Jia YAN Li-weia
(a.Department of Gynecology; b.CT/MRI Diagnosis, Affiliated Hospital of Hebei University, Hebei Baoding 071000, China)
关键词:
卵巢癌微小核糖核酸-128化疗效果
分类号:
R737.31;R730.43
DOI:
10.3969/j.issn.1671-7414.2022.06.019
文献标志码:
A
摘要:
目的 探讨卵巢癌患者血清微小核糖核酸(micro RNA,miR)-128 表达及与化疗效果及预后的相关性。方法 选取2012 年6 月~ 2017 年12 月在河北大学附属医院就诊的146 例卵巢癌患者为观察组,纳入患者均接受紫杉醇与卡铂药物联合治疗,另选取同期于该院进行健康体检的208 例成年女性作为对照组。对比两组临床资料,采用实时荧光定量PCR(qRT-PCR)检测两组受试者血清miR-128 表达水平,通过受试者工作特征曲线(ROC)确定血清miR-128 表达水平的最佳截断点,对观察组患者随访三年,采用Kaplan Meier 法绘制生存曲线,分析miR-128 不同表达水平患者的疗效及生存情况。采用二分类COX 回归分析筛选患者化疗效果不良的预测因素,并用rms 程序包建立列线图预测模型,采用校准曲线评价其预测效能。结果 观察组血清miR-128 表达水平(0.24±0.08)明显低于健康对照组(2.35±0.17),差异有统计学意义(t=139.48,P < 0.001);miR-128 低表达组患者化疗后疾病控制率明显低于miR-218 高表达组患者(40.95% vs 70.73%),差异具有统计学意义(χ2=10.461,P < 0.05);卵巢癌患者血清miRNA-128 表达水平与淋巴转移、FIGO 分期、分化程度和CA125 异常有关(χ2=28.303,33.241,48.107,22.889,均P<0.05);miRNA-128 低表达、淋巴转移、FIGO 分期III 期以上和肿瘤分化程度低、CA125 偏高是卵巢癌患者化疗效果不良的独立预测因素(OR=2.812,1.982,2.354,2.194,1.657,均P<0.05);miR-128 高表达患者三年生存率明显高于低表达患者(73.17%vs 44.76%),差异有统计学意义(Log-Rank χ2=9.548,P=0.002)。结论 MicroRNA-128 与卵巢癌的发生与发展密切相关,其表达水平对预测卵巢癌的化疗疗效和生存情况具有参考意义。
Abstract:
Objective To investigate the expression of serum miRNA-128 in patients with ovarian cancer and its correlation with chemotherapy effect and prognosis. Methods A total of 146 patients with ovarian cancer treated in the Affiliated Hospital of Hebei University from June 2012 to December 2017 were selected as the observation group. All the patients were treated with paclitaxel and carboplatin, and 208 adult women who underwent physical examination in the Hospital were selected as the control group. The clinical data of the two groups were compared, the serum miR-128 expression levels of the two groups of subjects were detected by real-time fluorescence quantitative PCR (qRT-PCR). The best cutoff point of serum miR-128 expression level was determined by the receiver operating characteristic curve (ROC). The patients in the observation group were followed up for three years. Kaplan Meier method was used to draw survival curves and analyze the efficacy and survival of patients with different miR-128 expression levels. Dichotomous COX regression analysis was used to screen the predictors of poor chemotherapy effect, and nomogram prediction model was established with RMS package, and its prediction efficiency was evaluated with calibration curve. Results The expression level of serum miR-128 in the observation group (0.24±0.08) was significantly lower than that of the healthy control group (2.35±0.17), and the difference was statistically significant (t=139.48, P < 0.001). The complete remission and disease control rate of patients in the miR-128 low expression group after chemotherapy were significantly lower than those in the miR-218 high expression group(40.95% vs 70.73%), and the difference was statistically significant(χ2=10.461,P < 0.05).The expression level of miR-128 in patients with ovarian cancer was related to lymphatic metastasis, FIGO staging, degree of differentiation, and abnormal CA125 (χ2=28.303, 33.241, 48.107, 22.889, all P<0.05). Low miR-128 expression, lymphatic metastasis, FIGO stage III or higher, low tumor differentiation, and high CA125 were independent predictors of poor chemotherapy effects in patients with ovarian cancer (OR=2.812, 1.982, 2.354, 2.194, 1.657, all P<0.05). The 3-year survival rate of patients with high miR-128 expression was significantly higher than that of patients with low expression (73.17% vs 44.76%),the difference was statistically significant (Log-Rank χ2=9.548, P=0.002). Conclusion MiRNA-128 was closely related to the occurrence and development of ovarian cancer, and its expression level has reference significance for predicting the efficacy and survival of ovarian cancer chemotherapy.

参考文献/References:

[1] ISHIKAWA M, IWASAKI M, ZHAO Hailin, et al. Sevoflurane and desflurane exposure enhanced cell proliferation and migration in ovarian cancer cells via miR-210 and miR-138 downregulation[J]. International Journal of Molecular Sciences, 2021, 22(4): 1826.
[2] FAHRMANN J F, IRAJIZAD E, KOBAYASHI M, et al. A MYC-Driven plasma polyamine signature for early detection of ovarian cancer[J]. Cancers, 2021, 13(4): 913.
[3] SALWA A, FERRARESI A, CHINTHAKINDI M, et al. BECN1 and BRCA1 deficiency sensitizes ovarian cancer to Platinum therapy and confers better prognosis[J]. Biomedicines, 2021, 9(2): 207.
[4] XU Lijun, ZHENG Qing. Identification and validation of a miRNA-related expression signature for tumor mutational burden in colorectal cancer[J]. World Journal of Surgical Oncology, 2021, 19(1): 56.
[5] LI Yang, LONG Xiaotao, WANG Ji, et al. miRNA-128 modulates bone neoplasms cells proliferation and migration through the WNT/β-catenin and EMT signal pathways[J]. Journal of Orthopaedic Surgery and Research, 2021, 16(1): 71.
[6] LIU Ping, HAN Ziping, MA Qingfeng, et al. Upregulation of microRNA-128 in the peripheral blood of acute ischemic stroke patients is correlated with stroke severity partially through inhibition of neuronal cell cycle reentry[J]. Cell Transplantation, 2019, 28(7): 839-850.
[7] VERNUCCIO F, GODFREY D, MEYER M, et al. Local tumor control and patient outcome using stereotactic body radiation therapy for hepatocellular carcinoma: iRECIST as a potential substitute for traditional criteria[J]. American Journal of Roentgenology, 2019, 213(6): 1232-1239.
[8] ROHR I, ALAVI S, RICHTER R, et al. Expectations and preferences of patients with primary and relapsed ovarian cancer to maintenance therapy: A NOGGO/ ENGOT-ov22 and GCIG survey (Expression IV)[J]. International Journal of Gynecological Cancer, 2020, 30(4): 509-514.
[9] STEFFEN T, H?LLER L, BIJELIC L, et al. Decision-making analysis for hyperthermic intraperitoneal chemotherapy in ovarian cancer:a survey by the executive committee of the peritoneal surface oncology group international(PSOGI)[J]. Oncology, 2021, 99(1): 41-48.
[10] ECKSTEIN M, SAILER V, NIELSEN B S, et al. Co-staining of microRNAs and their target proteins by miRNA in situ hybridization and immunohistofluorescence on prostate cancer tissue microarrays[J]. Laboratory Investigation, 2019, 99(10): 1527-1534.
[11] PISHKARI S, PARYAN M, HASHEMI M, et al. The role of microRNAs in different types of thyroid carcinoma: a comprehensive analysis to find new miRNA supplementary therapies[J]. Journal of Endocrinological Investigation, 2018, 41(3): 269-283.
[12] 侯娟,蒋树立,滕长财.基于 TCGA数据库卵巢癌患者的 miR-301b表达量与生存状况生物信息学分析 [J].现代检验医学杂志,2020,35(4): 37-40. HOU Juan, JIANG Shuli, TENG Changcai. Bioinformatics analysis of miR-301b expression and survival status of patients with ovarian cancer based on TCGA database [J]. Journal of Modern Laboratory Medicine, 2020, 35(4):37-40.
[13] TONG Deyong, ZOU Erwen, BAI Li, et al. IRF-1 mediated long non-coding RNA PVT1-214 promotes oxaliplatin resistance of colorectal cancer via miR-128 inhibition[J]. Journal of B.U.ON, 2020, 25(5): 2205-2214.
[14] CAO Danxia, ZHU Han, ZHAO Qian, et al. MiR-128 suppresses metastatic capacity by targeting metadherin in breast cancer cells[J]. Biological Research, 2020, 53(1): 43.
[15] 何哲炜,郭文坚.外泌体源性微小 RNA在急性髓细胞白血病中的研究现状 [J].国际输血及血液学杂志,2020,43(1):23-24. HE Zhewei, GUO Wenjian. Research status of exosome-derived microRNA in acute myeloid leukemia [J]. International Journal of Blood Transfusion and Hematology, 2020, 43(1):23-26.
[16] LIU Tong, ZHANG Xin, DU Lutao, et al. Exosome-transmitted miR-128-3p increase chemosensitivity of oxaliplatin-resistant colorectal cancer[J]. Molecular Cancer, 2019, 18(1): 43.
[17] ZENG Juan, LI Yukun, QUAN Feifei, et al. Propofol-induced miR-125a-5p inhibits the proliferation and metastasis of ovarian cancer by suppressing LIN28B[J]. Molecular Medicine Reports, 2020, 22(2): 1507-1517.
[18] WANG Junrong, HU Yubo, YE Cong, et al. miR-1224-5p inhibits the proliferation and invasion of ovarian cancer via targeting SND1[J]. Human Cell, 2020, 33(3): 780-789.
[19] ZHANG R R, WANG L M, SHEN J J. Over expression of miR-32 inhibits the proliferation and metastasis of ovarian cancer cells by targeting BTLA[J]. European Review for Medical and Pharmacological Sciences, 2020, 24(9): 4671-4678.
[20] CHEN Xiuhui, MANGALA L S, MOOBERRY L, et al. Identifying and targeting angiogenesis-related microRNAs in ovarian cancer[J]. Oncogene, 2019, 38(33): 6095-6108.

相似文献/References:

[1]顾益凤,朱自力,张金业,等.利用ROC曲线选择血清HE-4对卵巢癌的最佳诊断界点[J].现代检验医学杂志,2015,30(04):141.[doi:10.3969/j.issn.1671-7414.2015.04.043]
 GU Yi-feng,ZHU Zi-li,ZHANG Jin-ye,et al.Selecting the Best Cut-off Point of Serum HE-4 in Ovarian Cancer by ROC Curve[J].Journal of Modern Laboratory Medicine,2015,30(06):141.[doi:10.3969/j.issn.1671-7414.2015.04.043]
[2]郑福利,张颖,王建.血清S100A6检测对卵巢上皮性癌的诊断价值[J].现代检验医学杂志,2015,30(05):22.[doi:10.3969/j.issn.1671-7414.2015.05.007]
 ZHENG Fu-li,ZHANG Ying,WANG Jian.Study on Diagnostic Value of Detecting Serum S100A6 in the Epithelial Ovarian Carcinoma[J].Journal of Modern Laboratory Medicine,2015,30(06):22.[doi:10.3969/j.issn.1671-7414.2015.05.007]
[3]詹颉,常宁宁,李小侠,等.CEA,AFP,CA125,CA199和CA72-4联合检测在鉴别卵巢良恶性疾病中的应用价值[J].现代检验医学杂志,2015,30(05):50.[doi:10.3969/j.issn.1671-7414.2015.05.015]
 ZHAN Jie,CHANG Ning-ning,LI Xiao-xia,et al.Clinical Value of Combined Detection of CEA,AFP,CA125,CA199 and CA72-4 in the Identification of Ovarian Benign and Malignant Disease[J].Journal of Modern Laboratory Medicine,2015,30(06):50.[doi:10.3969/j.issn.1671-7414.2015.05.015]
[4]邢瑞青,吴永昌,陈慧昱,等.血清CA125,HE4及ROMA指数对卵巢癌诊断价值的临床研究[J].现代检验医学杂志,2016,31(06):37.[doi:10.3969/j.issn.1671-7414.2016.06.010]
 XING Rui-qing,WU Yong-chang,CHEN Hui-yu,et al.Diagnostic Value of Serum CA125,HE4 and ROMA Index in Patients with Ovarian Cancer[J].Journal of Modern Laboratory Medicine,2016,31(06):37.[doi:10.3969/j.issn.1671-7414.2016.06.010]
[5]张 悦a,段海霞,暴 蕾a,等.microRNA-125b在葛根素诱导人卵巢癌细胞SKOV3凋亡中的作用研究[J].现代检验医学杂志,2017,32(05):13.[doi:10.3969/j.issn.1671-7414.2017.05.004]
 ZHANG Yuea,DUAN Hai-xia,BAO Leia,et al.Role of MicroRNA-125b in Human Ovarian Cancer Cell SKOV3 Induced Apoptosis by Puerarin[J].Journal of Modern Laboratory Medicine,2017,32(06):13.[doi:10.3969/j.issn.1671-7414.2017.05.004]
[6]徐金良a,杜丹丹,翟志敏,等.卵巢良恶性肿瘤患者血液NLR,LMR,RDW,PLR四项参数的变化及其临床意义[J].现代检验医学杂志,2018,33(02):16.[doi:10.3969/j.issn.1671-7414.2018.02.001]
 XU Jin-lianga,DU Dan-dan,ZHAI Zhi-min,et al.Changes and Clinical Significance of Four Parameters of NLR,LMR, RDW and PLR in Patients with Benign and Malignant Ovarian Tumors[J].Journal of Modern Laboratory Medicine,2018,33(06):16.[doi:10.3969/j.issn.1671-7414.2018.02.001]
[7]马同敏,赵志强.血清CA125,STIP1和IGF-Ⅰ联合检测对卵巢癌的早期诊断价值研究[J].现代检验医学杂志,2018,33(06):50.[doi:10.3969/j.issn.1671-7414.2018.06.013]
 MA Tong-min,ZHAO Zhi-qiang.Clinical Value of Combined Detection of Serum CA125,STIP1 and IGF-I Levels in Early Diagnosis of Ovarian Cancer[J].Journal of Modern Laboratory Medicine,2018,33(06):50.[doi:10.3969/j.issn.1671-7414.2018.06.013]
[8]张善弟,荆成宝,禹 梅.血清CEA,CA125和HE4联合检测对卵巢癌的诊断价值[J].现代检验医学杂志,2018,33(06):122.[doi:10.3969/j.issn.1671-7414.2018.06.032]
 ZHANG Shan-di,JING Cheng-bao,YU Mei.Clinical Value of Combined Detection of Serum CEA,CA125 and HE4 in the Diagnosis of Ovarian Cancer[J].Journal of Modern Laboratory Medicine,2018,33(06):122.[doi:10.3969/j.issn.1671-7414.2018.06.032]
[9]刘 青,邓慧敏,周林涛,等.卵巢癌患者组织中miR-135b的表达及临床意义[J].现代检验医学杂志,2019,34(02):32.[doi:10.3969/j.issn.1671-7414.2019.02.009]
 LIU Qing,DENG Hui-min,ZHOU Lin-tao,et al.Expression and Clinical Significance of miR-135bin Tissues of Patients with Ovarian Cancer[J].Journal of Modern Laboratory Medicine,2019,34(06):32.[doi:10.3969/j.issn.1671-7414.2019.02.009]
[10]侯 娟,蒋树立,滕长财.基于TCGA 数据库卵巢癌患者的miR-301b 表达量与生存状况生物信息学分析[J].现代检验医学杂志,2020,35(04):37.[doi:10.3969/j.issn.1671-7414.2020.04.009]
 HOU Juan,JIANG Shu-li,TENG Chang-cai.Bioinformatics Analysis of Mir-301b Expression and Survival Status of Patients with Ovarian Cancer Based on TCGA Database[J].Journal of Modern Laboratory Medicine,2020,35(06):37.[doi:10.3969/j.issn.1671-7414.2020.04.009]

备注/Memo

备注/Memo:
基金项目:保定市科技计划项目(2041ZF309)
作者简介: 李品(1986-),女,硕士,主治医师,主要从事卵巢癌、宫颈癌、子宫内膜癌及盆腔子宫内膜异位症等妇科恶性肿瘤研究,E-mail:Pin504627144@163.com。
通讯作者:刘春晖(1985-),女,学士,主治医师,E-mail:Cherryliu1229@126.com。
更新日期/Last Update: 2022-11-15