[1]滕玲玲a,马广贞b,石 可a,等.重度子痫前期患者胎盘组织中CD44 mRNA和CD24 mRNA及蛋白表达水平的临床价值研究[J].现代检验医学杂志,2024,39(01):43-48.[doi:10.3969/j.issn.1671-7414.2024.01.008]
　TENG Linglinga,MA Guangzhenb,SHI Kea,et al.Clinical Value of CD44 mRNA and CD24 mRNA and Protein Expression Levels in Placental Tissue of Patients with Severe Preeclampsia[J].Journal of Modern Laboratory Medicine,2024,39(01):43-48.[doi:10.3969/j.issn.1671-7414.2024.01.008]
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重度子痫前期患者胎盘组织中CD44 mRNA和CD24 mRNA及蛋白表达水平的临床价值研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第39卷
期数:
2024年01期
页码:
43-48
栏目:
论著
出版日期:
2024-01-15

文章信息/Info

Title:
Clinical Value of CD44 mRNA and CD24 mRNA and Protein Expression Levels in Placental Tissue of Patients with Severe Preeclampsia
文章编号:
1671-7414（2024）01-043-06
作者:
滕玲玲1a; 马广贞1b; 石 可1a; 吕颖欣1a; 徐 静2
（1. 聊城市第二人民医院 a. 产科；b. 病理科，山东临清252601；2．河北医科大学第一医院产科，石家庄 050000）
Author(s):
TENG Lingling1a;  MA Guangzhen1b;  SHI Ke1a;  L? Yingxin1b;  XU Jing2
（1a.Department of Obstetrics; 1b.Department of Pathology, the Second People’s Hospital of Liaocheng, Shandong Linqing 252601, China; 2. Department of Obstetrics and Gynecology, the First Hospital of Hebei Medical University， Shijiazhuang 050000, China）
关键词:
重度子痫前期; 细胞表面跨膜糖蛋白分子44; 细胞表面跨膜糖蛋白分子24
分类号:
R714.245；R392.11
DOI:
10.3969/j.issn.1671-7414.2024.01.008
文献标志码:
A
摘要:
目的　探讨重度子痫前期（severe preeclampsia，SPE）患者胎盘中细胞表面跨膜糖蛋白分子（cell surfacetransmembrane glycoprotein molecules，CD）44 mRNA，细胞表面跨膜糖蛋白分子24（CD24） mRNA 及蛋白表达水平表达的临床价值研究。方法　选取2019 年6 月～ 2022 年6 月在聊城市第二人民医院接受剖宫产分娩的SPE 患者，根据发病孕龄的不同，进一步将其分为早发型SPE 组（孕龄≤ 34 周，n=45）和晚发型SPE 组（孕龄＞ 34 周，n=55）。选取同期产检正常者100 例为对照组。采用荧光定量PCR 和免疫组织化学检测SPE 患者胎盘中CD44 和CD24 表达，Pearson 法分析其表达水平差异以及与SPE 疾病临床特征的相关性，多因素Logistic 回归分析发生SPE 的影响因素。结果　相较于对照组，SPE 胎盘组织中CD44 mRNA（0.55±0.12 vs 1.02±0.33），CD24 mRNA 的表达水平（0.68±0.19vs 1.05±0.11）均降低，差异具有统计学意义（t=13.385，16.853，P ＜ 0.05）。免疫组织化学染色结果显示，CD44，CD24 在SPE 组胎盘组织中多呈阴性表达或弱阳性表达，而在对照组中多呈阳性表达，且SPE 胎盘组织中CD44，CD24 阳性率低于对照组，差异具有统计学意义（χ2=9.696，14.346，P ＜ 0.05）。相较于早发型SPE 组，晚发型SPE胎盘组织中CD44（0.65±0.17 vs 0.42±0.11），CD24（0.77±0.23 vs 0.58±0.13）mRNA 的表达水平均较高，差异具有统计学意义（t=7.830，4.932，P ＜ 0.05）。相较于对照组，SPE 组BMI，收缩压、舒张压、尿蛋白、Cr，LDH 和BUN均显著升高，差异有统计学意义（t=5.360 ～ 30.241，均P ＜ 0.05）；SPE 组分娩孕周较早、MPV，ALB 较低、新生儿出生身长较短和体质量较轻均低于对照组，差异具有统计学意义（t=3.232 ～ 11.109，均P ＜ 0.05）。且SPE 胎盘组织中CD44 与CD24 表达呈正相关（r=0.698，P ＜ 0.05），SPE 胎盘组织中CD44 的表达分别与CD24，分娩孕周、MPV和新生儿出生身长呈正相关（r=0.611，0.639，0.612，0.465，均P ＜ 0.05）；与收缩压、尿蛋白和LDH 呈负相关（r=-0.604，-0.569，-0.593，均P ＜ 0.05）。CD24 的表达分别与分娩孕周、MPV 和新生儿出生身长呈正相关（r=0.605，0.584，0.640，均P ＜ 0.05）；与收缩压、尿蛋白和LDH 呈负相关（r=-0.637，-0.593，-0.561，均P ＜ 0.05）。经Logistic回归分析结果显示，MPV（95%CI：1.429 ～ 4.350），尿蛋白（95%CI：1.529 ～ 2.709），LDH（95%CI：1.425 ～ 3.932）均是发生SPE 的独立危险因素（均P ＜ 0.05）。高水平CD44（95%CI：0.561 ～ 0.940），CD24（95%CI：0.495 ～ 0.814）是发生SPE 的独立保护因素（均P ＜ 0.05）。结论　SPE 患者胎盘中CD44，CD24 表达水平较低，高水平CD44，CD24 均是SPE 发生的独立保护因素，可为后续SPE 的治疗提供方向。
Abstract:
Objective　To explore clinical value of the expression levels of cell surface transmembrane glycoprotein molecule 44(CD44) mRNA, cell surface transmembrane glycoprotein molecule 24(CD24) mRNA, and protein in the placenta of severe preeclampsia(SPE) patients. Methods　The SPE patients who were delivered by cesarean section in the Second People’s Hospital of Liaocheng from June 2019 to June 2022 were further divided into 45 patients in early onset SPE group (gestational age ≤ 34 weeks) and 55 patients in late onset SPE group (gestational age>34 weeks) according to the different gestational age. The control group consisted of 100 normal cases in the same period. The expression of CD44 and CD24 in placenta of SPE patients was detected by fluorescent quantitative PCR and immunohistochemistry, Pearson method was used to analyze the difference of their expression levels and their correlation with the clinical characteristics of SPE disease, and multivariate logistic regression was used to analyze the influencing factors of SPE. Results　Compared with the control group, the expression levels of CD44 mRNA (0.55±0.12 vs 1.02±0.33) and CD24 mRNA (0.68±0.19 vs 1.05±0.11) in SPE placental tissues decreased significantly，the differences were statistically significant (t=13.385，16.853，P<0.05). The immunohistochemical staining results showed that CD44 and CD24 were mostly negative or weakly positive in the SPE group placental tissue, while they were mostly positive in the control group, the positive rates of CD44 and CD24 in the SPE placental tissue were lower than those in the control group, and the differences were statistically significant（χ2=9.696，14.346，P ＜ 0.05）.Compared to the early onset SPE group, the expression levels of CD44 (0.65 ± 0.17 vs 0.42 ± 0.11) and CD24 (0.77 ± 0.23 vs 0.58 ± 0.13) mRNA in placental tissue of late onset SPE were higher, and the differences were statistically significant (t=7.830，4.932，P<0.05). Compared with the control group, the BMI, systolic blood pressure, diastolic blood pressure, urinary protein, Cr, LDH and BUN were significantly increased in SPE group(t=5.360 ～ 30.241, all P ＜ 0.05). In SPE group, the gestational age was earlier, the MPV and ALB were lower, the newborn’s birth length was shorter, and the body weight than control group, the differences were statistically great (t=3.232 ～ 11.109，all P <0.05). The expression of CD44 and CD24 in SPE placenta was positively correlated (r=0.698, P<0.05), the expression of CD44 in SPE placenta was positively correlated with CD24，gestational week of delivery， MPV and neonatal birth length (r=0.611, 0.639, 0.612, 0.465, all P<0.05)，and was negatively correlated with systolic blood pressure, urinary protein and LDH (r=-0.604, -0.569, -0.593, all P<0.05). The expression of CD24 was positively correlated with gestational age, MPV and newborn birth length (r=0.605, 0.584, 0.640, all P<0.05)，and was negatively correlated with systolic blood pressure, urinary protein and LDH (r=-0.637, -0.593, -0.561, all P<0.05). The results of logistic regression analysis showed that MPV (95% CI: 1.429 ～ 4.350), urinary protein (95% CI: 1.529 ～ 2.709), and LDH (95% CI: 1.425 ～ 3.932) were all independent risk factors for SPE (all P<0.05). High levels of CD44 (95% CI: 0.561 ～ 0.940) and CD24 (95% CI: 0.495 ～ 0.814)were independent protective factors for SPE (P<0.05). Conclusion　The low expression levels of CD44 and CD24 in placenta of SPE patients are independent protective factors of SPE, which can provide direction for the follow-up treatment of SPE.

参考文献/References:

[1] KAWASAKI K, KONDOH E, CHIGUSA Y, et al. Metabolomic profiles of placenta in preeclampsia[J].Hypertension, 2019, 73(3): 671-679.
[2] GYSELAERS W, T HILAGANATHAN B . Preeclampsia: a gestational cardiorenal syndrome[J].Journal of Physiology, 2019, 597(18): 4695-4714.
[3] TURBEVILLE H R, SASSER J M. Preeclampsia beyond pregnancy: long-term consequences for mother and child[J]. American Journal of Physiology. Renal Physiology, 2020, 318(6): F1315-F1326.
[4] TOMIMATSU T, MIMURA K, MATSUZAKI S, et al. Preeclampsia: maternal systemic vascular disorder caused by generalized endothelial dysfunction due to placental antiangiogenic factors[J]. International Journal of Molecular Sciences, 2019, 20(17): 4246.
[5] ESSA A A M, DERAZ E M. Expression of CD44 (NKI-P1) in oral squamous cell carcinoma associated vascular endothelial cells: a relationship to tumor angiogenesis[J]. Saudi Dental Journal, 2022, 34(1): 21-26.
[6] ZOU Weiyan, YANG Yan, ZHENG Rongsheng, et al. Association of CD44 and CD24 phenotype with lymph node metastasis and survival in triple-negative breast cancer[J]. International Journal of Clinical and Experimental Pathology, 2020, 13(5): 1008-1016.
[7] 谢幸, 孔北华, 段涛. 妇产科学[M]. 9 版. 北京：人民卫生出版社, 2001: 83-84. XIE Xing, KONG Beihua, DUAN Tao. Obstetrics and Gynecology[M]. 9th Ed. Beijing：People’s Medical Publishing House, 2001: 83-84.
[8] 刘艳秋, 马小艳, 贺龙风, 等. 重度子痫前期胎盘组织中sVCAM-1, GDF-15 的表达及其临床意义[J]. 中国现代医学杂志, 2022, 32(17)：67-72. LIU Yanqiu, MA Xiaoyan, HE Longfeng, et al. Expression and clinical significance of sVCAM/1 and GDF/15 in placental tissues of severe preeclampsia[J].China Joumal of Modern Medicine, 2022, 32(17)：67-72.
[9] 尹杨雪, 徐琴, 廖灵蕴, 等. 重度子痫前期患者胎盘miR-651-3p 表达及与临床特征的关系[J]. 实用妇产科杂志, 2021, 37（9）：668-672. YIN Yangxue, XU Qin, LIAO Lingyun, et al. Expression level of miR-651-3p in placenta of patients with severe preeclampsia and its correlation with clinical characteristics [J]. Journal of Practical Obstetrics and Gynecology, 2021, 37(9): 668-672.
[10] LI Ting, ZHOU Bing, HE Yijing, et al. Expression and clinical diagnostic value of miR-383 in patients with severe preeclampsia[J]. Cellular and Molecular Biology (Noisy-le-Grand, france), 2020, 66(3): 92-100.
[11] 薛伟, 易福凌, 王苗, 等. 孕妇血清亲环素A 和组织型转谷氨酰胺酶水平检测与子痫前期发生不良妊娠的相关性研究[J]. 现代检验医学杂志, 2021, 36（6）：78-82. XUE Wei, YI Fuling, WANG Miao, et al. Correlation research between serum levels of cyclophilin a as well as tissue transglutaminase in pregnant women and adverse pregnancy in preeclampsia [J]. Journal of Modern Laboratory Medicine, 2021, 36(6): 78-82.
[12] 韩曦, 党群, 胡盈, 等. 重度子痫前期孕妇血清Endocan 表达水平及其对胎儿生长受限的预测价值[J]. 现代检验医学杂志, 2022, 37(6)：110-113, 170. HAN Xi, DANG Qun, HU Ying, et al. Study on the expression of endocan in serum of pregnant women with severe preeclampsia and its correlation with fetal growth restriction [J]. Journal of Modern Laboratory Medicine, 2022,37(6)：110-113, 170.
[13] TODD N, MCNALLY R, ALQUDAH A, et al. Role of a novel angiogenesis FKBPL-CD44 pathway in preeclampsia risk stratification and mesenchymal stem cell treatment [J]. Journal of Clinical Endocrinology & Metabolism, 2021, 106(1)：26-41.
[14] JASKU?A K, SACHARCZUK M, GACIONG Z, et al. Cardiovascular effects mediated by HMMR and CD44[J]. Mediators of Inflammation, 2021, 2021: 4977209.
[15] SHAPIRA S, KAZANOV D, DANKNER R, et al. High expression level of PPARγ in CD24 knockout mice and gender-specific metabolic changes: a model of insulin-sensitive obesity[J]. Journal of Personalized medicine, 2021, 11(1): 50.
[16] SAMMAR M, SIWETZ M, MEIRI H, et al. Reduced placental CD24 in preterm preeclampsia is an indicator for a failure of immune tolerance[J]. International Journal of Molecular Sciences, 2021, 22(15): 8045.
[17] BURWICK R M, PILLIOD R A, DUKHOVNY S E, et al. Fetal hydrops and the risk of severe preeclampsia[J].Journal of Maternal-Fetal & Neonatal Medicine, 2019, 32(6): 961-965.
[18] ONYANGUNGA O A, NAICKER T A, MOODLEY J. Maternal and perinatal outcomes after caesarean delivery in early and late onset preeclampsia with HIV positive and HIV negative south African women[J].Nigerian Journal of Clinical Practice, 2019, 22(5): 591-597.
[19] WALLE M, ASRIE F, GELAW Y, et al. The role of platelet parameters for the diagnosis of preeclampsia among pregnant women attending at the University of Gondar Comprehensive Specialized Hospital antenatal care unit, Gondar, Ethiopia[J]. Journal of Clinical Laboratory Analysis, 2022, 36(4): e24305.
[20] ?ALK S, YURTCU N, ?ETIN A. Predictive and diagnostic value of serum sVEGFR-1 level in women with preeclampsia: A prospective controlled study [J].Turkish Journal of Obstetrics and Gynecology, 2022, 19(4): 268-274.
[21] DUAN Zhongliang, LI Cui, LEUNG W T, et al. Alterations of several serum parameters are associated with preeclampsia and may be potential markers for the assessment of PE severity [J]. Disease Markers, 2020, 2020: 7815214.

相似文献/References:

[1]韩 曦a,党 群a,胡 盈a,等.重度子痫前期孕妇血清Endocan 表达水平及其对胎儿生长受限的预测价值[J].现代检验医学杂志,2022,37(06):110.[doi:10.3969/j.issn.1671-7414.2022.06.020]
　HAN Xia,DANG Quna,HU Yinga,et al.Study on the Expression of Endocan in Serum of Pregnant Women with Severe Preeclampsia and Its Correlation with Fetal Growth Restriction[J].Journal of Modern Laboratory Medicine,2022,37(01):110.[doi:10.3969/j.issn.1671-7414.2022.06.020]

备注/Memo

备注/Memo:
作者简介：滕玲玲(1976-)，女，本科，副主任医师，研究方向：围产保健，产科急危重症抢救，E-mail：rvj7kj@163.com。
通讯作者： 徐静(1983-)， 女， 硕士研究生， 副主任医师， 研究方向： 产科危急重症处理， 孕期保健与难产的处理，E-mail：qingchaotiao8@163.com。
更新日期/Last Update: 2024-01-15