[1]沈家亮,王 琳,尚文强.骨肉瘤患者癌组织中AHA1 mRNA 和LOXL2 mRNA 表达与侵袭转移基因mRNA 表达的相关性及临床意义[J].现代检验医学杂志,2024,39(02):39-45.[doi:10.3969/j.issn.1671-7414.2024.02.008]
　SHEN Jialiang,WANG Lin,SHANG Wenqiang.Correlation between the Expression of AHA1 mRNA and LOXL2 mRNA in Osteosarcoma Patients Tissues with mRNA Expression of Invasion and Metastasis Genes and Their Clinical Significance[J].Journal of Modern Laboratory Medicine,2024,39(02):39-45.[doi:10.3969/j.issn.1671-7414.2024.02.008]
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骨肉瘤患者癌组织中AHA1 mRNA 和LOXL2 mRNA 表达与侵袭转移基因mRNA 表达的相关性及临床意义()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第39卷
期数:
2024年02期
页码:
39-45
栏目:
论著
出版日期:
2024-03-31

文章信息/Info

Title:
Correlation between the Expression of AHA1 mRNA and LOXL2 mRNA in Osteosarcoma Patients Tissues with mRNA Expression of Invasion and Metastasis Genes and Their Clinical Significance
文章编号:
1671-7414（2024）02-039-07
作者:
沈家亮; 王 琳; 尚文强
（华北医疗健康集团峰峰总医院骨科，河北邯郸 056201）
Author(s):
SHEN Jialiang;  WANG Lin;  SHANG Wenqiang
(Department of Orthopedics，North China Medical and Health Group Fengfeng General Hospital，Hebei Handan 056021, China)
关键词:
骨肉瘤; 热休克蛋白90 ATP 酶激活因子1; 赖氨酰氧化酶样蛋白2; 肿瘤侵袭转移
分类号:
R738.1；R730.43
DOI:
10.3969/j.issn.1671-7414.2024.02.008
文献标志码:
A
摘要:
目的 研究骨肉瘤（osteosarcoma）患者癌组织中热休克蛋白90 ATP 酶激活因子1（the activator of HSP90ATPase-1，AHA1）和赖氨酰氧化酶样蛋白2（lysyl oxidase like-2 protein，LOXL2）表达与侵袭转移基因mRNA 表达的相关性及临床意义。方法 选取2016 年2 月~2017 年3 月华北医疗健康集团峰峰总医院诊治的90 例骨肉瘤患者为研究对象。应用实时荧光定量PCR 检测组织中AHA1 mRNA，LOXL2 mRNA 及侵袭转移基因Wnt 家族成员9A（Wnt9a）mRNA，锌指E 盒结合同源盒1（Zinc finger E-box binding homeobox 1，ZEB1）mRNA，锌指E 盒结合同源盒2（ZEB2）mRNA，N- 钙黏素（N-cad）mRNA 和波形蛋白（Vim）mRNA 表达。采用Pearson 相关分析，比较不同临床特征骨肉瘤患者AHA1 mRNA，LOXL2 mRNA 表达差异。Kaplan-Meier 生存分析影响AHA1 mRNA，LOXL2 mRNA 表达对骨肉瘤患者预后的影响。单因素及多因素COX 回归分析影响骨肉瘤患者预后的因素。结果 骨肉瘤组织中AHA1 mRNA（3.16 ± 0.59 ），LOXL2 mRNA（2.84 ± 0.44 ）及侵袭转移基因Wnt9a mRNA（3.23±0.42），ZEB1 mRNA（2.73±0.39），ZEB2 mRNA（2.52±0.56），N-cad mRNA（2.71±0.65），Vim mRNA（2.81±0.73）表达均高于癌旁组织（1.10 ±0.21，0.95 ± 0.18，0.79±0.15，0.64±0.11，0.98±0.19，0.68±0.14，0.72±0.15），差异具有统计学意义（t=31.206，37.716，51.903，48.931，24.706，28.964，26.605，均P<0.05）。骨肉瘤组织中AHA1 mRNA 与LOXL2 mRNA 表达呈显著正相关（r=0.712，P<0.05）。骨肉瘤组织中AHA1 mRNA，LOXL2 mRNA 与侵袭转移基因Wnt9a mRNA，ZEB1mRNA，ZEB2 mRNA，N-cad mRNA，Vim mRNA 表达呈显著正相关（r=0.504~0.720，均P<0.05）。Eneeking 分期Ⅲ期、有软组织浸润和有肺转移骨肉瘤组织中AHA1 mRNA，LOXL2 mRNA 表达高于Eneeking 分期Ⅰ ~ Ⅱ期、无软组织浸润和无肺转移患者，差异具有统计学意义(t=14.122~171.054，均P<0.05)。AHA1 mRNA 高表达组和低表达组患者五年生存率分别为36.36%（16/44），78.26%（36/46）。AHA1 mRNA 高表达组患者五年累积生存率明显低于低表达组，差异有统计学意义（Log-rank χ2=16.081，P<0.05）。LOXL2 mRNA 高表达组和低表达组患者五年生存率分别为34.88%（15/43），78.72%（37/47）。LOXL2 mRNA 高表达组患者五年累积生存率明显低于低表达组，差异有统计学意义（Log-rank χ2=15.880，P<0.05）。肺转移（OR=1.921，P<0.05），Eneeking 分期Ⅲ 期（OR=1.906，P<0.05），AHA1mRNA 高表达（OR=1.405，P<0.05），LOXL2 mRNA 高表达（OR=1.733，P<0.05）是影响骨肉瘤患者不良生存预后的独立危险因素。结论 骨肉瘤组织中AHA1 mRNA，LOXL2 mRNA 表达升高，两者表达与侵袭转移基因表达有关，是影响骨肉瘤患者不良生存预后的独立危险因素。
Abstract:
Objective To investigate the correlation between the expression of the activator of HSP90 ATPase-1 (AHA1), lysyl oxidase like-2 protein (LOXL2) in osteosarcoma tissues with mRNA expression of invasion and metastasis genes and their clinical significance. Methods A total of 90 osteosarcoma patients diagnosed and treated in North China Medical and Health Group Fengfeng General Hospital from February 2016 to March 2017 were selected as the research object. The expression of AHA1 mRNA, LOXL2 mRNA and invasion and metastasis genes Wnt family member 9A (Wnt9a) mRNA, zinc finger E-box binding homologous box 1 (ZEB1) mRNA, zinc finger E-box binding homologous box 1 (ZEB2) mRNA, N-cadherin (N-cad) mRNA, and vimentin (Vim) mRNA in tissues were detected by real-time fluorescence quantitative PCR. Pearson correlation analysis was used for correlation analysis. The differences in expression of AHA1 mRNA and LOXL2 mRNA in osteosarcoma patients among different clinical characteristics were compared. Kaplan-Meier survival analysis was used to analyze the effect of AHA1 mRNA and LOXL2 mRNA on the prognosis of osteosarcoma patients. The prognostic factors of osteosarcoma patients were analyzed by univariate and multivariate COX regression. Results The expressions of AHA1 mRNA (3.16 ± 0.59), LOXL2 mRNA (2.84 ± 0.44) and invasion and metastasis genes [Wnt9a mRNA（3.23±0.42）, ZEB1 mRNA（2.73±0.39）, ZEB2 mRNA（2.52±0.56）, N-cad mRNA（2.71±0.65） and Vim mRNA（2.81±0.73）] in osteosarcoma tissues were higher than those in paracancerous tissues（1.10 ± 0.21，0.95 ± 0.18，0.79±0.15，0.64±0.11，0.98±0.19，0.68±0.14， 0.72±0.15）, and the differences were statissically significant(t=31.206，37.716，51.903，48.931，24.706，28.964， 26.605，all P<0.05). There was a significant positive correlation between AHA1 mRNA and LOXL2 mRNA expression in osteosarcoma (r=0.712, P<0.05). The expressions of AHA1 mRNA and LOXL2 mRNA were significantly positively correlated with the expressions of invasion and metastasis genes (Wnt9a, ZEB1, ZEB2, N-cad, and Vim mRNA) in tumor tissue of osteosarcoma group (r=0.504~0.720, all P<0.05). The expressions of AHA1 mRNA and LOXL2 mRNA in osteosarcoma tissues with Eneeking stage III, soft tissue infiltration, and lung metastasis were higher than those in patients with Eneeking stage I~II, no soft tissue infiltration, and no lung metastasis, with significant differences (t=14.122~171.054, all P<0.05). The 5-year survival rates of patients in the AHA1 mRNA high expression group and low expression group were 36.36% (16/44) and 78.26% (36/46), respectively. The 5-year cumulative survival rate of patients in the AHA1 mRNA high expression group was significantly lower than that in the low expression group (Log-rank χ2=16.081, P<0.05). The 5-year survival rates of patients with high and low expression of LOXL2 mRNA were 34.88% (15/43) and 78.72% (37/47), respectively. The 5-year cumulative survival rate of patients in the LOXL2 mRNA high expression group was significantly lower than that in the low expression group (Log-rank χ2=15.880, P<0.05). Lung metastasis (OR=1.921, P<0.05), Eneeking stage III (OR=1.906, P<0.05), AHA1 mRNA high expression (OR=1.405, P<0.05), and LOXL2 mRNA high expression (OR=1.733, P<0.05) were independent risk factors affecting the poor survival prognosis of osteosarcoma patients. Conclusion The expressions of AHA1 mRNA and LOXL2 mRNA in osteosarcoma were increased, and they were correlated with the expression of invasion and metastasis genes, indicating they may be independent risk factors affecting the poor survival and prognosis of osteosarcoma patients.

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备注/Memo

备注/Memo:
基金项目： 河北省“三三三人才工程”资助项目（C20221129）：骨肉瘤组织中AHA1 和LOXL2 表达与侵袭转移基因表达的相关性及临床意义研究。
作者简介：沈家亮（1983-），男，硕士，主治医师，研究方向：骨肿瘤，E-mail：sjl19831210@163.com。
通讯作者：尚文强（1981-），男，本科，副主任医师，研究方向：骨与关节、骨肿瘤，E-mail：153896276@qq.com。
更新日期/Last Update: 2024-03-15