[1]陈 敏,沈亚卉.非小细胞肺癌组织中CLDN10 和CSPG4 水平表达与临床病理特征及预后的关系研究[J].现代检验医学杂志,2024,39(02):46-50+74.[doi:10.3969/j.issn.1671-7414.2024.02.009]
　CHEN Min,SHEN Yahui.Study on the Relationship between CLDN10 and CSPG4 Levels Expression of Tissue and Clinicopathological Characteristics and Prognosis in Non-small Cell Lung Cancer[J].Journal of Modern Laboratory Medicine,2024,39(02):46-50+74.[doi:10.3969/j.issn.1671-7414.2024.02.009]
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非小细胞肺癌组织中CLDN10 和CSPG4 水平表达与临床病理特征及预后的关系研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第39卷
期数:
2024年02期
页码:
46-50+74
栏目:
论著
出版日期:
2024-03-31

文章信息/Info

Title:
Study on the Relationship between CLDN10 and CSPG4 Levels Expression of Tissue and Clinicopathological Characteristics and Prognosis in Non-small Cell Lung Cancer
文章编号:
1671-7414（2024）02-046-06
作者:
陈 敏; 沈亚卉
（南京医科大学附属泰州市人民医院呼吸与危重症医学科，江苏泰州 225300）
Author(s):
CHEN Min;  SHEN Yahui
（Department of Respiratory and Critical Care Medicine, Taizhou People’s Hospital, Nanjing Medical University, Jiangsu Taizhou 225300, China）
关键词:
非小细胞肺癌; 紧密连接蛋白10; 硫酸软骨素蛋白聚糖4
分类号:
R734.2；R730.43
DOI:
10.3969/j.issn.1671-7414.2024.02.009
文献标志码:
A
摘要:
目的　探讨非小细胞肺癌（non-small cell lung cancer，NSCLC） 组织中紧密连接蛋白10（claudin 10，CLDN10）和硫酸软骨素蛋白聚糖4（chondroitin sulfate proteoglycan 4，CSPG4）表达与临床病理特征和预后的关系。方法　随机选取2017 年1 月~2018 年4 月于南京医科大学附属泰州市人民医院进行手术的136 例NSCLC 患者为研究对象，并根据随访结局（生存或死亡）分为生存组（n=63）和死亡组（n=69）。收集136 例患者于手术过程中切除的癌组织及癌旁组织标本；实时荧光定量PCR（real-time fluorescence quantitative PCR，RT-qPCR）检测癌组织及癌旁组织CLDN10 和CSPG4 表达水平，并分别以NSCLC 患者癌组织中CLDN10 和CSPG4 表达水平的平均数为界限，分为CLDN10 高表达组（n=66），CLDN10 低表达组（n=70）和CSPG4 高表达组（n=71），CSPG4 低表达组（n=65）；采用多因素COX 回归分析确定NSCLC 患者预后的影响因素；采用Kaplan-Meier 生存曲线分析NSCLC 患者CLDN10和CSPG4 表达水平与其预后的关系。结果　NSCLC 组织中CLDN10 表达水平（0.96 ± 0.25）低于癌旁组织（1.73 ±0.42），CSPG4 表达水平（1.80 ± 0.46）高于癌旁组织（1.04 ± 0.27），差异有统计学意义（t=18.372，16.617，均P＜ 0.05）。与生存组比较，死亡组低未分化占比、淋巴结转移占比及癌组织中CSPG4 表达水平较高，CLDN10 表达水平较低，差异具有统计学意义（t/χ2=8.463，7.423，11.696，6.426，均P ＜ 0.05）。CLDN10 高表达组五年存活率（58.46%）高于低表达组（37.31%），CSPG4 低表达组患者五年存活率（55.56%）高于高表达组（40.58%），差异有统计学意义（χ2=7.848，4.018，均P ＜ 0.05）。多因素COX 分析结果显示，CLDN10 低表达、CSPG4 高表达、低分化程度、淋巴结转移是NSCLC 患者预后的危险因素（HR=1.362，1.368，1.335，1.314，均P ＜ 0.05）。结论　NSCLC 患者癌组织中CLDN10 表达水平较低，CSPG4 水平较高，且与NSCLC 发展和预后密切相关。
Abstract:
Objective　To investigate the relationship between the expression of claudin 10 (CLDN10) and chondroitin sulfate proteoglycan 4 (CSPG4) of tissue and clinicopathologic characteristics and prognosis in non-small cell lung cancer (NSCLC). Methods　A total of 136 NSCLC patients who underwent surgery in Taizhou People’s Hospital, Nanjing Medical University from January 2017 to April 2018 were randomly selected as the study subjects, and these subjects were grouped into survival group (n=63) and death group (n=69) based on follow-up outcomes (survival or death). Specimens of cancer tissue and adjacent tissues excised during surgery were collected from 136 patients. Real-time fluorescence quantitative PCR (RT-qPCR) was applied to detect the expression levels of CLDN10 and CSPG4 in cancer tissue and adjacent tissues. Based on the average expression levels of CLDN10 and CSPG4 in cancer tissues of NSCLC patients, they were grouped into CLDN10 high expression group (n=66), CLDN10 low expression group (n=70) and CSPG4 high expression group (n=71), CSPG4 low expression group (n=65). Multivariate COX regression analysis was used to determine the prognostic factors of NSCLC patients. Kaplan-Meier survival curve was applied to analyze the relationship between the expression levels of CLDN10 and CSPG4 in NSCLC patients and their prognosis. Results　The expression level of CLDN10 (0.96 ± 0.25) in NSCLC tissues was lower than that in adjacent tissue (1.73 ± 0.42), and the expression level of CSPG4 in NSCLC tissues (1.80 ± 0.46) was higher than that in adjacent tissues (1.04 ± 0.27), with statistical significance (t=18.372, 16.617, all P < 0.05). Compared with the survival group, the proportion of low undifferentiated, lymph node metastasis and CSPG4 expression of cancer tissues in the death group were higher, and the CLDN10 expression level of cancer tissues in the death group was lower, with statistical significance (t/χ2=8.463, 7.423, 11.696, 6.426, all P < 0.05). The five-year survival rate of the high expression group of CLDN10 (58.46%) was higher than that of the low expression group (37.31%), and the five-year survival rate of the low expression group of CSPG4 (55.56%) was higher than that of the high expression group (40.58%), and the differences were statistically significant (χ2=7.848, 4.018, all P < 0.05). Multivariate COX analysis results showed that low expression of CLDN10, high expression of CSPG4, low differentiation degree, and lymph node metastasis were all risk factors affecting the prognosis of NSCLC patients (HR=1.362, 1.368, 1.335, 1.314, all P<0.05). Conclusion　The expression level of CLDN10 was low and CSPG4 was high in cancer tissues of NSCLC patients, which was closely related to the development and prognosis of NSCLC.

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备注/Memo

备注/Memo:
作者简介：陈敏（1990-），女，硕士研究生，主治医师，研究方向：呼吸系统疾病，E-mail：ch3371f@163.com。
通讯作者：沈亚卉（1985-），女，博士研究生，副主任医师，研究方向：呼吸系统疾病基础与临床研究，E-mail：shenyahui@njmu.edu.cn。
更新日期/Last Update: 2024-03-15