[1]高 珺,王 琼,林广裕,等.病毒性脑炎患儿脑脊液人类鼻病毒检测的价值[J].现代检验医学杂志,2015,30(02):11-15.[doi:10.3969/j.issn.1671-7414.2015.02.004]
　GAO Jun,WANG Qiong,LIN Guang-yu,et al.Value of Human Rhinovirus Detected in Cerebrospinal Fluid of Children with Viral Encephalitis[J].Journal of Modern Laboratory Medicine,2015,30(02):11-15.[doi:10.3969/j.issn.1671-7414.2015.02.004]
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病毒性脑炎患儿脑脊液人类鼻病毒检测的价值()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年02期
页码:
11-15
栏目:
论著
出版日期:
2015-03-20

文章信息/Info

Title:
Value of Human Rhinovirus Detected in Cerebrospinal Fluid of Children with Viral Encephalitis
作者:
高 珺1; 王 琼2; 林广裕3; 陈洁玲3; 陆学东2
1.广东医学院,广东湛江 524023; 2.广东医学院附属福田医院医学检验部,广东深圳 518033; 3.汕头大学医学院第二附属医院儿科,广东汕头 515041
Author(s):
GAO Jun1; WANG Qiong2; LIN Guang-yu3; CHEN Jie-ling3; LU Xue-dong2
1.Guangdong Medical College,Guangdong Zhanjiang 524023,China; 2.Department of Laboratory Medicine,Affiliated Futian Hospital of Guangdong Medical College Guangdong Shenzhen 518033,China; 3.Department of Pediatrics,the SecondAffiliated Hospital of Shantou University Medical College,Guangdong Shantou 515041,China
关键词:
鼻病毒;  病毒性脑炎;  儿童;  巢式逆转录聚合酶链反应
分类号:
R512.3; R373.19
DOI:
10.3969/j.issn.1671-7414.2015.02.004
文献标志码:
A
摘要:
目的 了解儿童病毒性脑炎的病原体,建立快速、特异检测鼻病毒的方法,探讨病毒性脑炎与鼻病毒的相关性。方法 收集2012年1月～12月全年期间收住在汕头大学医学院第二附属医院儿科重症监护室出现发热、惊厥症状的患儿脑脊液标本169份,应用巢式RT-PCR扩增脑脊液标本的鼻病毒核酸,对阳性结果进行核酸序列测定和同源性分析。结果 巢式RT-PCR总共检测出39份鼻病毒阳性样本,总阳性检出率为23.1%; 人类鼻病毒(HRV)感染的儿童中年龄在5岁以下的占87.6%; 7～9月份HRV阳性检出率较高; 序列分析显示39例HRV检测阳性标本中HRV-A型18例(46.1%,18/39),HRV-B型7例(17.9%,7/39),HRV-C型14例(35.9%,14/39); 28例病毒性脑炎有8例检出HRV,其中3例为HRV-C。结论 建立的巢式RT-PCR可以检测到脑脊液标本中的鼻病毒片段,并且HRV-A,B和C三型均有检出,结合临床症状可认为HRV可能是病毒性脑炎的病原体之一。
Abstract:
Objective To understand the pathogen of viral encephalitis(VE)in children,and establish a rapid and specific method for detecting human rhinovirus(HRV),and investigate the correlation between HRV andviral encephalitis(VE).Methods 169 CSF specimens were collected from children with convulsions and fever,who were admitted to the pediatric intensive care unit(PICU)of Second Affiliated Hospital of Shantou University Medical College between January 2012 and December 2012.Nested RT-PCR was used to detected HRV in CSF specimens,and the positive PCR products were sequenced,then analyzed and constructed the phylogenetic tree by software.Results 39(23.1%)out of 169 samples were HRV positive.Among them,148(87.6%)children were below 5 years old.The detection rate of HRV increases from July to September,and reached its highest point in September.Sequence analyzed showed that the 39 HRV positive specimens including 18(46.1%,18/39)positive for HRV-A,7(17.9%,7/39)positive for HRV-B,14(35.9%,14/39)positive for HRV-C.There were 8 out of 28 VE cases were detected in HRV,including 3(50%,3/6)positive for HRV-C.Conclusion HRV could be detected in CSF specimens by nested RT-PCR,including three types of HRV,combined with clinical symptoms considered that HRV may be one of the VE pathogen.

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备注/Memo

备注/Memo:
作者简介:高 珺(1989-),女,研究生在读,临床检验诊断学,Tel:13723483437,E-mail:junegaoo@163.com。 通讯作者:陆学东,研究员,E-mail:luxuedong2004@163.com。
更新日期/Last Update: 2015-03-20