[1]陈 瑾,郭彩利,孙春红,等.采用不同血管通路采集自体外周血造血干细胞的安全性及有效性评估[J].现代检验医学杂志,2015,30(04):111-114.[doi:10.3969/j.issn.1671-7414.2015.04.032]
　CHEN Jin,GUO Cai-li,SUN Chun-hong,et al.Safety and Efficacy of Adopting Different Vascular Access During Autologous Peripheral Blood Stem Cells Collection[J].Journal of Modern Laboratory Medicine,2015,30(04):111-114.[doi:10.3969/j.issn.1671-7414.2015.04.032]
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采用不同血管通路采集自体外周血造血干细胞的安全性及有效性评估()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年04期
页码:
111-114
栏目:
研究简报·实验技术
出版日期:
2015-08-10

文章信息/Info

Title:
Safety and Efficacy of Adopting Different Vascular Access During Autologous Peripheral Blood Stem Cells Collection
文章编号:
1671-7414(2015)04-111-04
作者:
陈 瑾; 郭彩利; 孙春红; 刘 芳; 王 艳; 王晓宁
西安交通大学第一附属医院,西安 710061
Author(s):
CHEN Jin; GUO Cai-li; SUN Chun-hong; LIU Fang; WANG Yan; WANG Xiao-ning
the First Affiliated Hospital of Xi'an Jiaotong University,Xi'an 710061,China
关键词:
造血干细胞采集;  双腔股静脉置管;  单个核细胞
分类号:
R457
DOI:
10.3969/j.issn.1671-7414.2015.04.032
文献标志码:
A
摘要:
目的 探讨采用不同血管通路采集自体外周血造血干细胞的安全性及有效性。方法 2012年1月～2014年11月期间行自体外周血造血干细胞采集的87例患者分为两组:外周静脉采集组43例,均采用16G一次性内瘘针进行穿刺,进血管路选用肘部静脉,返血管路选用大隐静脉。中心静脉采集组44例,均采用双腔股静脉置管建立静脉通路。自体外周血造血的采集均选用Cobe Spectra血细胞分离机的Spectra Auto PBSC操作程序进行。观察两组患者在穿刺成功率、采集顺利程度、患者术中耐受度、采集过程中的不良反应以及最终采集物中单个核细胞数和CD34+细胞计数的差异。结果 两组患者采集的自体外周血单个核细胞数及CD34+细胞计数间差异无统计学显著性意义(t=4.159,3.147,P＞0.05),且均采集达标; 采集过程中不良反应在两组患者中差异无统计学显著性意义(χ2=0.000 9,P＞0.05); 但采用中心静脉组患者穿刺成功率高、采集顺利、患者术中耐受性均高于采用外周静脉通路的患者,差异有统计学意义(χ2=7.944,13.772 9,20.509 4,P＜0.05)。结论 采用双腔股静脉置管建立中心静脉通路在自体外周血干细胞采集中具有穿刺成功率高,采集顺利,最大程度减轻了患者的痛苦,且不影响采集效率和效果的优势。
Abstract:
Objective To assess the safety and efficacy ofadopting different vascular access during autologous peripheral blood stem cellscollection.Methods 87 patients received autologous peripheral blood stem cells collection were divided into two groups:One was peripheral vein harvesting group(43 cases),which used the 16G disposable fistula needle for autologous peripheral blood stem cell collection and the other central venous harvesting group(44 cases),which used double cavity of femoral vein catheter for autologous peripheral blood stem cell collection.The observation indicators included venous accesses,collection efficiency,patient tolerance,thenumber of mononuclear cell and CD34 positive cells.Results The numbers of mononuclear cells and CD34 positive cells in two groups were all above the standard and there was no significant difference(P＞0.05).However,the success of venous accesses,the efficiency smooth of collection and-patient tolerance were better in double cavity of femoral vein catheter group(P＜0.05).Conclusion Harvesting the autologous peripheral blood stem cell through central venous by using double cavity of femoral veincatheter had the advantages as follows:high success rate of puncture,acquisition smoothly and reducing the suffering extent of the patients,and also it did not affect the acquisition efficiency and effectiveness.

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备注/Memo

备注/Memo:
作者简介:陈 瑾(1965-),女,本科,学士,主管护师,曾任血液科护士长,研究方向:外周血干细胞采集,Tel:13700281799,E-mail:xueye35@126.com。
通讯作者:王晓宁。
更新日期/Last Update: 2015-08-10