[1]吴健,崔蕾蕾,刘连庚.姜黄素对皮肤鳞状细胞癌A431细胞侵袭的抑制作用[J].现代检验医学杂志,2015,30(05):62-65.[doi:10.3969/j.issn.1671-7414.2015.05.019]
 WU Jian,CUI Lei-lei,LIU Lian-geng.Inhibitory Effect of Curcumin on Invasion of Skin Squamous Cell Carcinoma A431 Cells[J].Journal of Modern Laboratory Medicine,2015,30(05):62-65.[doi:10.3969/j.issn.1671-7414.2015.05.019]
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姜黄素对皮肤鳞状细胞癌A431细胞侵袭的抑制作用()

《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年05期
页码:
62-65
栏目:
论著
出版日期:
2015-12-10

文章信息/Info

Title:
Inhibitory Effect of Curcumin on Invasion of Skin Squamous Cell Carcinoma A431 Cells
作者:
吴健1崔蕾蕾1刘连庚2
1.盐城市第一人民医院检验科,江苏盐城224005;2.盐城市中医院检验科,江苏盐城224002
Author(s):
WU Jian1CUI Lei-lei1LIU Lian-geng2
1.Department of Laboratory Medicine,the First People’s Hospital of Yancheng City, Jiangsu Yancheng 224005,China;2.Department of Laboratory Medicine, Yancheng Hospital of Traditional Chinese Medicine,Jiangsu Yancheng 224002,China
关键词:
姜黄素皮肤鳞状细胞癌侵袭信号传导和转录激活因子3侵袭
分类号:
R739.5;R730.43
DOI:
10.3969/j.issn.1671-7414.2015.05.019
文献标志码:
A
摘要:
目的研究姜黄素对侵袭信号传导和转录激活因子3(signal transducer and activator of transcription 3,STAT3)表达的调控,初步探讨姜黄素在防治皮肤鳞状细胞癌侵袭转移中可能存在的新的作用机制。方法以高侵袭性皮肤鳞癌细胞株A431为研究对象,采用不同剂量姜黄素处理。MTT法检测5,10,15,20,25,30,35,40和50 μmol/L姜黄素作用24,48和72 h对细胞的毒性作用;Transwell小室侵袭试验法检测5,10和15 μmol/L姜黄素对细胞的侵袭能力,黏附试验法检测对细胞的黏附能力;Western blot法检测5,10和15 μmol/L姜黄素作用后STAT3蛋白表达水平的影响,RT-PCR法检测对细胞中STAT3基因mRNA转录水平的影响。结果姜黄素浓度<15 μmol/L,作用时间24 h时,对细胞的生长抑制呈时间和剂量依懒性(P>0.001),当姜黄素浓度15 μmol/L,作用时间为24 h时,对细胞无明显毒性作用,细胞存活率85%;细胞侵袭能力和黏附能力随着姜黄素浓度的增加而逐渐降低,以15 μmol/L姜黄素浓度处理效果最明显(P>0.05);姜黄素可明显抑制STAT3基因mRNA转录水平,其抑制作用与剂量呈高度依懒性(P>0.05)。结论姜黄素可通过抑制STAT3信号通路的活化及该信号通路靶基因STAT3的表达降低皮肤鳞癌A431细胞的侵袭性。
Abstract:
ObjectiveTo investigate the regulatory effect of curcumin on expression of signal transducer and activator of transcription 3 (STAT3) in skin squamous cell carcinoma tissues as well as the possible mechanism of curcumin in prevention and treatment of skin squamous cell carcinoma.MethodsHighly invasive A431 cells were treated with curcumin at various dosages.The cytotoxic effects of treatment with 5,10,15,20,25,30,35,40 and 50 μmol/L curcumin for 24,48 and 72 h on A431 cells were measured by MTT assay.The invasion abilities of cells treated with 5,10 and 15 μmol/L curcumin were measured by Transwell test,while the adhesive abilities by cell adhesion assay.The effect of 5,10 and 15 μmol/L curcumas on expression levels of STAT3 were determined by western blot,while those on transcription level of STAT3 mRNA by RT-PCR.ResultsThe treatment with curcumin at a dosage of more than 15 μmol/L for more than 24 h inhibited the growth of A431 cells in time-and dose-dependent patterns (P<0.001).The treatment with curcumin at a dosage of not less than 15 μmol/L for 24 h showed no significant cytotoxic effect on the cells,while the survival rate of cells was more than 85%.The invasion and adhesive abilities of cells decreased gradually with the increasing curcumin concentration.The curcumin at a concentration of 15 μmol/L showed the strongest inhibitory effect on the two abilities (P<0.05).Curcumin showed significantly dose-dependent inhibitory effect on the transcription level of STAT3 mRNA (P<0.05).ConclusionCurcumin inhibited the invasive abilitiy of A431 cells by inhibiting the activation of STAT3 signal pathway and expression of STAT3 as a target gene of the pathway.

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备注/Memo

备注/Memo:
作者简介:吴健(1979-),男,硕士在读,主管检验师,主要从事肿瘤分子诊断方向的研究,E-mail:piaoxue1982717@sina.com。 通讯作者:刘连庚(1970-),男,副主任检验师,主要从事肿瘤诊断研究E-mail:wujianglinxing@163.com。
更新日期/Last Update: 1900-01-01