[1]吴瑞,杨杨,朱贞,等.2013年西安地区风疹病毒的分离与基因特征研究[J].现代检验医学杂志,2015,30(05):84-86.[doi:10.3969/j.issn.1671-7414.2015.05.025]
　WU-Rui,YANG-Yang,ZHU-Zhen,et al.Genetic Characteristics of Rubella Virus Strains Isolated in Xi’an City，2013[J].Journal of Modern Laboratory Medicine,2015,30(05):84-86.[doi:10.3969/j.issn.1671-7414.2015.05.025]
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2013年西安地区风疹病毒的分离与基因特征研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年05期
页码:
84-86
栏目:
论著
出版日期:
2015-12-10

文章信息/Info

Title:
Genetic Characteristics of Rubella Virus Strains Isolated in Xi’an City，2013
作者:
吴瑞1; 杨杨1; 朱贞2; 司源3; 刘继锋1; 马超锋1
1.西安市疾病预防控制中心，西安710054； 2.中国疾病预防控制中心，北京100050；3.陕西省疾病预防控制中心，西安710054
Author(s):
WU-Rui1; YANG-Yang1; ZHU-Zhen2; SI-Yuan3; Liu Ji-feng1; MA Chao-feng1
1.Xi’an Center for Disease Control and Prevention，Xi’an 710074，China； 2.Chinese Center for Disease Control and Prevention，Beijing 100050，China； 3.Shaanxi Provincial Center for Disease Control and Prevention，Xi’an 710054，China
关键词:
西安地区; 风疹病毒; 基因型别; 基因特征
分类号:
R373.11；R372
DOI:
10.3969/j.issn.1671-7414.2015.05.025
文献标志码:
A
摘要:
目的了解西安地区风疹病毒基因特征，为风疹控制工作提供分子流行病学的基线数据。方法采集西安地区2013年3起农村学校风疹暴发疫情中27个病例的27份咽拭子标本及6份尿液标本，利用RT-PCR法检测风疹病毒核酸，阳性者接种Vero-SLAM细胞分离病毒，并利用RT-PCR方法鉴定分离到的毒株，对分离到的风疹病毒进行基因序列测定，利用BIOEDIT，MAGA6.0软件进行风疹病毒基因特征分析。结果27份咽拭子标本中共25份风疹核酸阳性，6份尿液标本均为风疹核酸阳性；从风疹核酸阳性标本分离病毒，25份咽拭子标本和6份尿液标本分别分离到18株和5株风疹病毒株。9株测序成功，经鉴定全部属于1E型基因型。结论2013年西安地区分离到的风疹病毒株与我国的主要基因型一致，应加强对西安地区风疹血清学诊断、病毒学和血清流行病学监测。
Abstract:
ObjectiveTo investigate the genetic characteristics of rubella virus strains isolated in Xi’an city，then to provide molecular epidemiological?baseline data for further control．MethodsA total of 27 cases of pharyngeal swab specimens and 6 cases of urine specimens were collected from rubella outbreaks patients in 3 rubella outbreak in 2013．RT-PCR method was used to detect rubella virus nucleic acid，rubella virus isolated by Vero-SLAM cells and identified by RT-PCR method．The genetic characteristics were analyzed with the virus isolating and RT-PCR method combining．The virus strains were amplified by RT-PCR and sequenced．Phylogenetic tree was constructed by using BIOEDIT and MEGA 4.0 software package．ResultsAmong 27 swab specimens and 6 cases of urine specimens there were 25 and 4 RUV RNA positive．18 strains of rubella virus strain isolated from 23 RUV RNA positive (swab specimens) and 5 strains from 6 (urine specimens) specimens in xi’an region，but only 9 rubella virus strains via sequencing appraisal all belong to genotype 1E genotype．Conclusion1E genotype of rubella virus is the predominance genotype in Xi’an city．Should strengthen the rubella serological diagnosis，virology and serum epidemiological surveillance．

参考文献/References:

［1］刘继锋，相晓妹，马超锋，等．2004～2012年西安市风疹流行病学分析［J］．现代预防医学，2015，42(2)：208-210． Liu JF，Xiang XM，Ma CF，et al．Analysis of the epidemiological characteristics of rubella in Xi’an city from 2004～2012［J］．Modern Preventive Medicine，2015，42(2)：208-210．
[2]Wang Y，Ma Y，Xu XT，et al．Genetic characterization analysis on epidemic rubella virus strains isolated in Liaoning from 2007 to 2012［J］．Bing Du Xue Bao，2013，29(6)：589-595．
[3]Zhu Z，Cui A，Wang H，et al．Emergence and continuous evolution of genotype 1E rubella viruses in China［J］．J Clin Microbiol，2012，50(2)：353-363．
[4]朱贞，郭学斌，崔爱利，等．中国2007～2008年风疹流行病学和病毒基因特征分析［J］．中国疫苗和免疫，2009，15(3)：201-204． Zhu Z，Guo XB，Cui AL，et al．Analysis of epidemiological and genetic characteristics of rubella viruses in china from 2007 to 2008［J］．Chinese Journal of Vaccines and Immunization，2009，15(3)：201-204．
[5]马静，罗会明，郝利新，等．中国2005～2011年风疹流行病学特征分析［J］．中国疫苗和免疫，2012，18(6)：500-503，540． Ma J，Luo HM，Hao LX，et al．Analysis on epidemiological characteristics of rubella in China during 2005～2011［J］．Chinese Journal of Vaccines and Immunization，2012，18(6)：500-503，540．
[6]马玉杰，薄芳，黄鹤，等．黑龙江省消除麻疹阶段风疹流行特征分析［J］．中国公共卫生管理，2015，31(1)：71-73． Ma YJ，Bo F，Huang H，et al．Analysis of characteristics of rubella epidemic in measles elimination phase，in Heilongjiang province［J］．China Journal Public Health Management，2015，31(1)：71-73．
[7]朱贞，许文波，蒋小泓，等．2003～2007年中国风疹病毒基因特征分析［J］．病毒学报，2008，24(1)：7-16． Zhu Z，Xu WB，Jiang XH，et al．Genetic characterization of Chinese rubella virus isolates from 2003～2007［J］．Chinese Journal of Virology，2008，24(1)：7-16．
[8]Chen M，Zhu Z，Liu D，et al．Rubella epidemic caused by genotype 1E rubella viruses in Beijing，China，in 2007～2011［J］．Virol J，2013(10)：122．
[9]杜慧，郭玉，赵娜，等．河北省风疹病毒的分离鉴定及基因型分析［J］．河北医药，2012，34(21)：3315-3316． Du H，Guo Y，Zhao N，et al．Rubella virus isolation and identification of genotype analysis in hebei province［J］．Hebei Medical Journal，2012，34(21)：3315-3316．

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备注/Memo

备注/Memo:
作者简介：吴瑞(1982-)，女，博士，主管检验技师，研究方向：病毒病分子诊断，Tel：029-85520062，E-mail：wur2003@163.com。 通讯作者：马超锋(1974-)，男，博士，副主任医师，E-mail：mark7447@tom.com。
更新日期/Last Update: 1900-01-01