[1]梁 涛,张 杨,许依婷,等.哮喘患者外周血单个核细胞TIM-3及Galectin-9的表达与临床意义[J].现代检验医学杂志,2016,31(01):52-54.[doi:10.3969/j.issn.1671-7414.2016.01.014]
 LIANG Tao,ZHANG Yang,XU Yi-ting,et al.Express of TIM-3 and Galectin-9 Genes in Peripheral Blood Mononuclear Cells from Patients with Asthma[J].Journal of Modern Laboratory Medicine,2016,31(01):52-54.[doi:10.3969/j.issn.1671-7414.2016.01.014]
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哮喘患者外周血单个核细胞TIM-3及Galectin-9的表达与临床意义()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年01期
页码:
52-54
栏目:
论著
出版日期:
2016-01-25

文章信息/Info

Title:
Express of TIM-3 and Galectin-9 Genes in Peripheral Blood Mononuclear Cells from Patients with Asthma
作者:
梁 涛张 杨许依婷王 宁蔡鹏程王晓蓓胡丽华
华中科技大学同济医学院附属协和医院检验科,武汉 430022
Author(s):
LIANG TaoZHANG YangXU Yi-tingWANG NingCAI Peng-chengWANG Xiao-bei HU Li-hua
Department of Clinical Laboratory,Union Hospital, Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China
关键词:
哮喘 T细胞免疫球蛋白黏蛋白3 半乳糖凝集素-9
分类号:
R562.25; R392.11
DOI:
10.3969/j.issn.1671-7414.2016.01.014
文献标志码:
A
摘要:
目的 探讨T细胞免疫球蛋白黏蛋白3(TIM-3)和半乳糖凝集素-9(Galectin-9)基因在急性发作期哮喘患者外周血单个核细胞的表达及临床意义。方法 收集2014年1月~10月门诊急性发作期哮喘患者60例(排除不符合者15例,确定入选45例)及30例健康体检者(对照组),以实时定量RT-PCR法分别对哮喘患者及体检者外周血单个核细胞(PBMCs)中的TIM-3,Galectin-9和IFN-γ的表达情况进行同步测定,初步探讨TIM-3,Galectin-9在哮喘发病中的潜在作用。结果 急性发作期哮喘患者PBMCs中TIM-3,Galectin-9及IFN-γ mRNA水平均分别高于健康对照组(U=458.5,P=0.019; U=437.5,P=0.010; U=260,P<0.001),二者间的差异均有统计学意义。结论 TIM-3/Galectin-9可能参与了哮喘的发生、发展,TIM-3或(和)Galectin-9有可能为治疗哮喘提供一个新的靶点。
Abstract:
Objective To investigate the levels of the mRNAexpression of TIM-3 and Galectin-9 in peripheral blood monocytes(PBMCs)of acute exacerbation asthma patients and their clinical significances.Methods 60 patients with acute exacerbation asthma(eliminating 15 cases of non-conform to the regulations)and 30 cases of healthy subjects were collected from January to October of 2014.Used fluorescence quantitative real-time reverse transcription-polymerase chain reaction to measure the mRNA expression of TIM-3 and Galectin-9 in PBMCs of patients with asthma and healthy controls.Results The expression of TIM-3,Galectin-9 and IFN-γ mRNA in the PBMCs from acute exacerbation asthma patients were all abnormally higher than healthy controls(U=458.5,P=0.019; U=437.5,P=0.010; U=260,P<0.001).There were statistically significant differences between them.Conclusion TIM-3/Galectin-9 pathway may participate inthe occurrence,development of asthma.TIM-3 or(and)Galectin-9 may prove tobe an important target for treatments to asthma.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:武汉市科技攻关计划项目,项目编号:2013060602010235。 作者简介:梁 涛(1983-),男,硕士研究生,技师,从事临床免疫研究。 通讯作者:胡丽华,Tel:027-85726311,E-mail:lihuahu@hust.edu.cn。
更新日期/Last Update: 2016-01-20