[1]孙灵迪a,王平均b,鹿 永a,等.HLA-B27基因表达量及亚型分析在AS疾病评估中的意义[J].现代检验医学杂志,2016,31(01):58-60.[doi:10.3969/j.issn.1671-7414.2016.01.016]
　SUN Ling-dia,WANG Ping-junb,LU Yonga,et al.Significance of the Expression Quantity of HLA-B27 Gene and Its Subtype in Estimating Patients with Ankylosing Spondylitis[J].Journal of Modern Laboratory Medicine,2016,31(01):58-60.[doi:10.3969/j.issn.1671-7414.2016.01.016]
点击复制

HLA-B27基因表达量及亚型分析在AS疾病评估中的意义()
分享到：

《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年01期
页码:
58-60
栏目:
论著
出版日期:
2016-01-25

文章信息/Info

Title:
Significance of the Expression Quantity of HLA-B27 Gene and Its Subtype in Estimating Patients with Ankylosing Spondylitis
作者:
孙灵迪a; 王平均b; 鹿 永a; 武晓茜a; 邵先安a
解放军第123医院a.检验病理科; b.脊柱外科,安徽蚌埠 233015
Author(s):
SUN Ling-dia; WANG Ping-junb; LU Yonga; WU Xiao-qiana; SHAO Xian-ana
a.Clinical Laboratory and Pathological Department; b.Surgical Department,the 123 Hospital of PLA,Anhui Bengbu 233015,China
关键词:
强直性脊柱炎;  人类白细胞抗原-B27;  基因;  表达量;  疾病活动指数
分类号:
R593.23; Q786
DOI:
10.3969/j.issn.1671-7414.2016.01.016
文献标志码:
A
摘要:
目的 探讨人类白细胞抗原(human leukocyto antigens,HLA)B27(HLA-B27)基因表达量及其亚型在强直性脊柱炎疾病诊疗中的意义。方法收集120例疑似强直性脊柱炎患者和50例健康体检者的临床资料,对所收集患者的全血采用实时荧光定量聚合酶链反应检测HLA-B27基因相对表达量,在此基础上通过基因测序确认HLA-B27基因亚型。采用SPSS17.0软件对数据进行分析,P<0.05被认为差异有统计学意义。结果 120例疑似强直性脊柱炎患者中,确诊55例,其中HLA-B27阳性53例(96.36%),BASDAI评分与HLA-B27基因表达量具有相关性(r=0.845,P=0.000)。基因亚型分别为HLA-B27:04型29例(54.72%),HLA-B27:05型20例(37.74%),HLA-B27:02型2例(3.77%),HLA-B27:03型1例(1.89%),HLA-B27:07型1例(1.89%)。50例健康对照组中筛查HLA-B27阳性2例(4%),均为B27:04亚型。对HLA-B27:04和HLA-B27:05两种亚型患者年龄(t=0.711,P=0.480)、性别(χ2=0.880,P=0.348)、家族史(χ2=0.011,P=0.916)和治疗史(χ2=0.113,P=0.736)比较差异无统计学意义。结论 HLA-B27阳性AS患者以HLA-B27:04和HLA-B27:05为主。HLA-B27基因表达量与其疾病活动指数相关。
Abstract:
Objective To investigate whether expression quantity of HLA-B27 and its subtypes associated with incidence of AS,the gene expression of HLA-B27 and its subtypes were detected in patients with AS.Methods 120 cases patients suspected with AS and 50 healthy subjects were enrolled in the study.Main demographic and clinical characteristics ofthe subjects were collected.Meanwhile total RNA was isolated from peripheral blood and real time RT-PCR was used to measure the quantitative expression of HLA-B27 gene.Besides RT-PCR,sequence-based typing(SBT)method was used to confirm the HLA-B27 subtype.All experimental data were analyzed by SPSS17.0 software and P values <0.05 were considered to be significant.Results Firstly,there were 55 subjects were finally diagnosed as AS patients among the 120 patients suspected with AS.There were 53 subjects whose HLA-B27 was positive(96.36%)in 55 patients with AS.It showed that there was acorrelation between BASDAI and expression quantity of HLA-B27 gene(r=0.845,P=0.000).Five subtypes were found and which were HLA-B27:04 subtype(29/53,54.72%),HLA-B27:05 subtype(20/53,37.74%),HLA-B27:02 subtype(2/53,3.77%),HLA-B27:03 subtype(1/53,1.89%)and HLA-B27:07 subtype(1/53,1.89%),respectively.Among the 50 healthy subjects,there were only one kind of subtype(2/50,4%),which was HLA-B27:04.There were no statistical difference in the age(t=0.711,P=0.480),sex(χ2=0.880,P=0.348),family history(χ2=0.011,P=0.916)and treatment(χ2=0.113,P=0.736)between the HLA-B27:04 and HLA-B27:05 subtypes.ConclusionHLA-B27:04 and HLA-B27:05 were primary subtypes in AS patients which HLA-B27 positive.There was a correlation between gene expression quantityof HLA-B27 and AS disease activity index.

参考文献/References:

[1] Dean LE,Jones GT,MacDonald AG,et al.Global pre-valence of ankylosing spondylitis[J].Rheumatology,2014,53(4):650-657.
[2] Robinson PC,Brown MA.The genetics of ankylosing spondylitis and axial spondyloarthritis[J].Rheum Dis Clin North Am,2012,38(3):539-553.
[3] Jiang Y,Yang M,Wu H,et al.The relationship between disease activity measured by the BASDAI and psychological status,stressful life events,and sleep quality in ankylosing spondylitis[J].Clinical Rheumatology,2015,34(3):503-510.
[4] Zambrano-Zaragoza JF,Agraz-Cibrian JM,González-Reyes C,et al.Ankylosing spondylitis:from cells to genes[J].International Journal of Inflammation,2013(50):1653.
[5] Raychaudhuri SP,Deodhar A.The classification and diagnostic criteria ofankylosing spondylitis[J].J Autoimun,2014(48/49):128-133.
[6] Johnsen SS,Bakland G,Nossent JC.The distribution of HLA-B27 subtype in patients with ankylosing spondylitis in Northern Norway[J].Scandinavian Journal of Rheumatology,2014,43(4):296-300.
[7] Ng EK,Chong WW,Jin H,et al.Differential expression of microRNAS in plasma of patients with colorectal cancer:a potential marker for colorectal cancer screening[J].Gut,2009,58(10):1375-1381.
[8] 张志坚,奚永志,孙玉英.全基因组单核苷酸多态性与强直性脊柱炎遗传易感性的研究进展[J].中华医学杂志,2011,91(18):1289-1291. Zhang ZJ,Xi YZ,Sun YY.Progress of genetic susceptibility of the whole genome single nucleotide polymorphism and ankylosing spondylitis[J].NatlMed J China,2011,91(18):1289-1291.
[9] 杜旭娜,李 晏,张胜利,等.比较BASDAI和mini-BASDAI对强直性脊柱炎病情的判断价值[J].军医进修学院学报,2012,33(6):556-558. Du XN,Li Y,Zhang SL,et al.Value of bath ankylosing spondylitis disease activity index and mini-bath ankylosing spondylitis disease activity index for diagnosis of ankylosing spondylitis[J].Journal of Chinese PLA Postgraduate Medical School,2012,33(6):556-558.
[10] 孙灵迪,梅传忠,鹿 永,等.PCR-SBT与IMS-ELI-SA法在强直性脊柱炎患者HLA-B27检测中的比较[J].现代检验医学杂志,2015,30(2):16-18. Sun LD,Mei CZ,Lu Y,et al.Comparison analysis of HLA-B27 detection by PCR-SBT and IMS-ELISA in AS patients[J].J Mod Lab Med,2015,30(2):16-18.

相似文献/References:

[1]王 森,高 硕,徐学静,等.强直性脊柱炎患者外周血单个核细胞中Toll样受体表达及功能的研究[J].现代检验医学杂志,2016,31(02):60.[doi:10.3969/j.issn.1671-7414.2016.02.018]
　WANG Sen,GAO Shuo,XU Xue-jing,et al.Expression Profiles and Function of Toll-like Receptors in Peripheral Blood Mononuclear Cells of Ankylosing Spondylitis Patients[J].Journal of Modern Laboratory Medicine,2016,31(01):60.[doi:10.3969/j.issn.1671-7414.2016.02.018]
[2]王平均a,孙灵迪b,武晓茜b,等.HLA-B27表达量在评估强直性脊柱炎患者疾病状况中的作用[J].现代检验医学杂志,2015,30(05):153.[doi:10.3969/j.issn.1671-7414.2015.05.049]
　WANG Ping-juna,SUN Ling-dib,WU Xiao-qianb,et al.Roles of the Expression Intensity of HLA-B27 in Estimating Patients with Ankylosing Spondylitis[J].Journal of Modern Laboratory Medicine,2015,30(01):153.[doi:10.3969/j.issn.1671-7414.2015.05.049]
[3]张　静a,董松武b,孙　峰a.ROC 曲线设置流式细胞术检测 HLA-B27 的临界值在强直性脊柱炎诊断和鉴别诊断中的应用[J].现代检验医学杂志,2020,35(06):153.[doi:doi:10.3969/j.issn.1671-7414.2020.06.037]
　ZHANG Jing a,DONG Song-wu b,SUN Feng a.Application of ROC Curve of Setting HLA-B27 Critical Value in theDiagnosis and Differential Diagnosis of Ankylosing Spondylitis[J].Journal of Modern Laboratory Medicine,2020,35(01):153.[doi:doi:10.3969/j.issn.1671-7414.2020.06.037]
[4]王　佩,米晓燕,白园园,等.强直性脊柱炎患者血清 YKL-40，Galectin-3 和 ChT 水平与疾病活动度及动脉粥样硬化进展的相关性研究[J].现代检验医学杂志,2021,36(03):52.[doi:10.3969/j.issn.1671-7414.2021.03.012]
　WANG Pei,MI Xiao-yan,BAI Yuan-yuan,et al.Correlation of Serum YKL-40, Galectin-3, CHT Levels with Disease Activityand Progression of Atherosclerosis in Patients with Ankylosing Spondylitis[J].Journal of Modern Laboratory Medicine,2021,36(01):52.[doi:10.3969/j.issn.1671-7414.2021.03.012]
[5]曹俊杰a,罗改莹a,王　瑞b.外周血C-反应蛋白与清蛋白比值及红细胞分布宽度检测对强直性脊柱炎的诊断价值[J].现代检验医学杂志,2021,36(06):136.[doi:10.3969/j.issn.1671-7414.2021.06.029]
　CAO Jun-jie,LUO Gai-ying,WANG Rui.Clinical Value of Peripheral Blood C-reactive Protein to Albumin Ratio and Red Blood Cell Distribution Width in Diagnosis of Ankylosing Spondylitis[J].Journal of Modern Laboratory Medicine,2021,36(01):136.[doi:10.3969/j.issn.1671-7414.2021.06.029]
[6]严鸣光,方 晓,李汶轩,等.强直性脊柱炎模型小鼠踝关节组织和临床患者PBMC 中miR-142-5p，SOCS1 mRNA 表达及与免疫功能分析研究[J].现代检验医学杂志,2024,39(03):29.[doi:10.3969/j.issn.1671-7414.2024.03.005]
　YAN Mingguang,FANG Xiao,LI Wenxuan,et al.Expression of miR-142-5p, SOCS1 mRNA in Ankle Joint Tissue Spondylitis Model Mice and PBMC of Clinical Patients and Their Immune Function Analysis[J].Journal of Modern Laboratory Medicine,2024,39(01):29.[doi:10.3969/j.issn.1671-7414.2024.03.005]

备注/Memo

备注/Memo:
基金项目:南京军区医学科技课题资助项目(12MB015)。 作者简介:孙灵迪(1986-),男,硕士生,研究方向:分子免疫诊断。Tel:18305523929。 通讯作者:邵先安,男,副教授,硕士生导师,研究方向:分子/基因免疫。E-mail:XAShao@126.com。
更新日期/Last Update: 2016-01-20