[1]张 蕾a,刘挺挺a,叶 辛b,等.IL-33在隐球菌脑膜炎患者外周血单个核中的表达及临床意义[J].现代检验医学杂志,2016,31(02):29-31.[doi:10.3969/j.issn.1671-7414.2016.02.009]
 ZHANG Leia,LIU Ting-Tinga,YE Xinb,et al.Expression of IL-33 in Patients with Cryptococcal Meningitis and Its Clinical Significance[J].Journal of Modern Laboratory Medicine,2016,31(02):29-31.[doi:10.3969/j.issn.1671-7414.2016.02.009]
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IL-33在隐球菌脑膜炎患者外周血单个核中的表达及临床意义()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年02期
页码:
29-31
栏目:
论著
出版日期:
2016-06-01

文章信息/Info

Title:
Expression of IL-33 in Patients with Cryptococcal Meningitis and Its Clinical Significance
作者:
张 蕾1a刘挺挺1a叶 辛1b陈孙孝2邓安梅1a
1.第二军医大学长海医院a.实验诊断科; b.输血科,上海 200433; 2.第二军医大学长征医院皮肤科,上海 200003
Author(s):
ZHANG Lei1aLIU Ting-Ting1aYE Xin1bCHEN Sun-xiao2DENG An-mei1a
1a.Department of Laboratory Diagnosis; 1b.Department of Transfusion Medicine,ChanghaiHospital, the Second Military Medical University,Shanghai 200433,China; 2.Department of Dermatology, Changzheng Hospital,the Second Military Medical University,Shanghai 2
关键词:
隐球菌脑膜炎 白细胞介素-33
分类号:
R519.4; R392.11
DOI:
10.3969/j.issn.1671-7414.2016.02.009
文献标志码:
A
摘要:
目的 探讨IL-33在隐球菌脑膜炎患者中的表达。方法 纳入上海长海医院和长征医院2008年5月~2014年9月隐球菌脑膜炎患者28例,健康对照30例。分离外周血单个核细胞,采用实时荧光定量PCR检测IL-33 mRNA表达量,ELISA法检测血清中IL-33蛋白浓度; 同时采用流式细胞术检测外周血中Th1/Th2细胞亚群的百分比,并分析其与血清中IL-33浓度的相关性。结果 隐球菌脑膜炎患者外周血单个核细胞及血清中IL-33水平明显增高(2.1±0.9 vs 1.0±0.2,U=136.0,P<0.001; 121.2±59.2 vs 56.7±19.7,U=141.0,P<0.001); 外周血中Th1细胞比例降低(4.1±1.7 vs 5.9±1.6,U=191.0,P<0.001),Th2细胞比例增高(2.8±0.9 vs 2.2±0.8,U=288.0,P<0.05); 进一步分析发现血清中IL-33浓度与Th2细胞百分比正相关,差异具有统计学意义(R=0.69,P<0.001)。结论 IL-33可能参与了隐球菌脑膜炎的发病机制。
Abstract:
Objective To explore the expression of IL-33 in patients with cryptococcal meningitis.Methods 28 patients with cryptococcal meningitis admitted to Shanghai Changhai and Changzheng Hospital and 30 healthy controls from May 2008 to September 2014 were enrolled.Peripheral blood mononuclear cells(PBMCs)were separated.IL-33 mRNA expressionwas determination by quantitative real-time PCR.Serum IL-33 level was determined by ELISA.The percentage of Th1 and Th2 cells was detected by flow cytometry,and their relation with serum IL-33 was analyzed.Results IL-33 mRNA and protein level were both increased in patients with ryptococcal meningitis(2.1±0.9 vs 1.0±0.2,U=136.0,P<0.001; 121.2±59.2vs 56.7±19.7,U=141.0,P<0.001),compared to healthy controls.The percentage of Th1 cells(4.1±1.7 vs 5.9±1.6,U=191.0,P<0.001)was decreased and Th2 cells(2.8±0.9 vs 2.2±0.8,U=288.0,P<0.05)was increased,compared to healthy controls.Furthermore, serum IL-33 level was positively correlated with Th2 cells(R=0.69,P<0.001).ConclusionIL-33 may be involved in the pathogenesis of cryptococcal meningitis.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:973计划[2013(B531606)]; 国家自然科学基金(81471605,81302579,81300748,81373282,81202353,81401358); 上海申康基金(CHDC22014014),军队科研基金(BWS14J023),长海医院1255学科建设计划(CH125530300),南京军区医药卫生科研基金(12MA056)。 作者简介:张 蕾(1990-),女,在读研究生,主要从事感染免疫研究。刘挺挺(1989-),女,在读研究生,主要从事感染免疫研究,为共同第一作者
更新日期/Last Update: 2016-02-20