[1]王京伟,李 艳,童永清,等.湖北地区非小细胞肺癌EGFR 基因突变及其意义的研究[J].现代检验医学杂志,2016,31(03):7-11.[doi:10.3969/j.issn.1671-7414.2016.03.003]
　WANG Jing-wei,LI Yan,TONG Yong-qing,et al.Detection of Epidermal Growth Factor Receptor(EGFR) Mutations and the Significance in Patients with Non-small Cell Lung Cancer(NSCLC)of Hubei Province[J].Journal of Modern Laboratory Medicine,2016,31(03):7-11.[doi:10.3969/j.issn.1671-7414.2016.03.003]
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湖北地区非小细胞肺癌EGFR 基因突变及其意义的研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年03期
页码:
7-11
栏目:
论著
出版日期:
2016-06-15

文章信息/Info

Title:
Detection of Epidermal Growth Factor Receptor(EGFR) Mutations and the Significance in Patients with Non-small Cell Lung Cancer(NSCLC)of Hubei Province
文章编号:
1671-7414(2016)03-007-06
作者:
王京伟; 李 艳; 童永清; 吴 薇; 郑红云
武汉大学人民医院检验科,武汉 430060
Author(s):
WANG Jing-wei; LI Yan; TONG Yong-qing; WU Wei; ZHENGHong-yun
Department of Clinical Laboratory,Renmin Hospital of Wuhan University,Wuhan 430060,China
关键词:
非小细胞肺癌;  EGFR突变;  PCR-sanger测序;  ARMS扩增
分类号:
R734.2; R730.43
DOI:
10.3969/j.issn.1671-7414.2016.03.003
文献标志码:
A
摘要:
目的 探讨湖北地区非小细胞肺癌(NSCLC)患者中EGFR基因的突变情况,评价湖北地区EGFR突变的检测力度,为临床合理选择酪氨酸激酶抑制剂提供可靠的实验依据。方法 采用PCR-sanger测序法以及ADx-ARMS法检测2010～2015年期间253例肺癌标本中EGFR基因突变情况,探讨不同检测手段的敏感度,探讨不同性别、不同组织分型间EGFR不同外显子的突变频率差异及其临床意义。结果253例肺癌标本中共检测到突变位点93个,以E19和E21的突变最为常见,占比为53.76%和35.38%。ADx-ARMS法的EGFR总突变检出率明显高于Sanger测序法(P=0.001)。女性肺癌患者的检出率明显高于男性(P=0.001)。不同组织分型中,腺癌(38.01%)的检出率最高,腺鳞癌(30.77%)与大细胞癌(20%)次之,鳞癌(4.55%)较低。无吸烟史的肺癌患者检出率(51.6%,81/157)明显高于有吸烟史患者(24%,12/50)。结论与PCR-sanger测序法相比,ADx-ARMS法对临床筛选EGFR-TKI治疗的受益亚群,预测EGFR-TKI疗效更为敏感有意义。湖北地区女性肺癌患者中EGFR突变频率明显高于男性,与激素水平变化及吸烟状况有关。
Abstract:
Objective To evaluated the prevalence of EGFR mutations in Hubei region,to provide reliable experimental basis for reasonable screening TKI beneficiaries in clinic.Methods EGFR mutation of 253 patients diagnosed with NSCLC in Hubei region during 2010 to 2015 was detected by PCR-sanger sequencing and ARMS assay,toinvestigate the sensitivity of different methods,to explore the frequency andclinical significance of EGFR mutation in different gender,in different histological type and different exons.Results 93 of 253 NSCLC patients harbored EGFR mutation,mainly occurred in exon 19 and 21,accounting for 53.76% and 35.38% of the total EGFR mutation rate,respectively.ADx-ARMS method showed higher sensitivity(P=0.001).The mutation detection rate of female NSCLC was significantly higher than that of male NSCLC(P=0.001).Theobserved incidence of EGFR mutations in patients with adenocarcinomas(38.01%)was the highest in different histological type,followed by glandular squamouscell carcinoma(30.77%),large cell carcinoma(20%)and squamous cell carcinoma(4.55%).Nonsmoking patients had a higher EGFR mutation frequency(51.6%,81/157)than those with a history of smoking(24%,12/50).ConclusionARMS assay was more sensitive and more convenient detection method for clinical screening for EGFR-TKI treatment subpopulation.The mutation rate offemale NSCLC was significantly higher than that of male NSCLC in Hubei Province.Speculated that the sexual differences in NSCLC with EGFR mutation frequencieswere related to hormone levels and smoking status.

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备注/Memo

备注/Memo:
基金项目:国家自然基金项目(81200389,8110059),武汉大学青年教师自主科研项目(2042015Kf0113)。 作者简介:王京伟(1986-),女,博士,主治医师,主要从事分子诊断与个体化医疗,动脉粥样硬化的分子机制,泛素化蛋白质组学研究,Tel:17771860355,E-mail:sophia wang03@hotmail.com。 通讯作者:李 艳,E-mail:yanlitf120@yahoo.com.cn。
更新日期/Last Update: 2016-06-25