[1]岳金芳a,帕提古丽·吾甫尔b.慢性阻塞性肺疾病患者miR-21表达与IL-12水平关系探讨[J].现代检验医学杂志,2016,31(03):153-155.[doi:10.3969/j.issn.1671-7414.2016.03.043]
　YUE Jin-fanga,PATIGULI·Wufuerb.Discussion on the Relationship of the miR-21 Expression and IL-12 Level in Patients with Chronic Obstructive Pulmonary Disease[J].Journal of Modern Laboratory Medicine,2016,31(03):153-155.[doi:10.3969/j.issn.1671-7414.2016.03.043]
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慢性阻塞性肺疾病患者miR-21表达与IL-12水平关系探讨()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年03期
页码:
153-155
栏目:
检验与临床
出版日期:
2016-06-15

文章信息/Info

Title:
Discussion on the Relationship of the miR-21 Expression and IL-12 Level in Patients with Chronic Obstructive Pulmonary Disease
作者:
岳金芳a; 帕提古丽·吾甫尔b
新疆喀什地区第一人民医院a.重症医学一科;
b.普内科,新疆喀什 844000
Author(s):
YUE Jin-fanga; PATIGULI·Wufuerb
a.Intensive Medical Department;
b.Internal Medicine the First People's Hospital of Kashi,Xinjiang Kashi 844000,China
关键词:
miR-21;  IL-12;  慢性阻塞性肺疾病
分类号:
R563; R392.11
DOI:
10.3969/j.issn.1671-7414.2016.03.043
文献标志码:
A
摘要:
目的 探讨慢性阻塞性肺疾病(COPD)患者外周血单个核细胞miR-21表达和血清IL-12水平的关系。方法 选取COPD患者共80例,对照组50例为健康人群,通过实时荧光定量PCR检测外周血单个核细胞miR-21表达,酶联免疫吸附法检测血清IL-12水平。结果 COPD稳定期组、急性期组和对照组间血糖、胆固醇和三酰甘油水平差异均无统计学意义(F=0.341～1.542,均P>0.05),而miR-21表达和IL-12水平比较差异有统计学意义(F=8.951～15.017,均P<0.05)。COPD稳定期组和急性期组miR-21表达和IL-12水平均比对照组高,差异有统计学意义(t=10.501～25.612,均P<0.05)。COPD急性期组比稳定期组miR-21表达和IL-12水平高,且差异有统计学意义(t=13.298～36.415,均P<0.05)。IL-12和miR-21存在显著的正相关(r=0.381～0.496,均P<0.05)。结论 miR-21可能参与调控IL-12的水平,miR-21表达与COPD的发病机制相关。
Abstract:
Objective To discuss the relationship of miR-21 expression and IL-12 level in patients with chronic obstructive pulmonarydisease.Methods Chosed 80 patients with suspected chronic obstructivepulmonary disease(COPD)and 50 cases as control group. Real-time RT-PCR was performed to determine the expression 1evels of miR-21 of peripheral blood mononuclear cells of three groups.To detect IL-12 level by ELISA method.Results There were no statistical difference among GLU,TC and TGlevel among stable phage,acute phage of COPD and control group(F=0.341～1.542,P>0.05).However miR-21 expression and IL-12 level had statisticaldifference(F=8.951～15.017,P<0.05).The expression 1evels of miR-21 of peripheral blood mononuclear cells of acute phage group were apparently higherthan stable phage,health group(P<0.05).The concentration of IL-12 in serum of acute COPD group was higher than other two groups(P<0.05),comparedwith stable COPD group and health group.IL-12 level was positiveiy related tomiR-21 expression(r=-0.381～0.496).Conclusion miR-2lmay be involved in the regulation of IL-12 expression.miR-21expression may be closely related to the pathogenesis of COPD.

参考文献/References:

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备注/Memo

备注/Memo:
作者简介:岳金芳(1981-),女,主治医师,主要从事临床重症疾病的诊疗工作。
更新日期/Last Update: 2016-06-25