[1]章晓燕,王 薇,何法霖,等.全国网织红细胞计数 参考区间和决定限现状调查与分析[J].现代检验医学杂志,2016,31(03):158-161.[doi:10.3969/j.issn.1671-7414.2016.03.045]
　ZHANG Xiao-yan,WANG Wei,HE Fa-lin,et al.Investigation and Analysis of Reference Intervals and Decision Limits of Reticulocyte Count in China[J].Journal of Modern Laboratory Medicine,2016,31(03):158-161.[doi:10.3969/j.issn.1671-7414.2016.03.045]
点击复制

全国网织红细胞计数 参考区间和决定限现状调查与分析()

《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年03期
页码:
158-161
栏目:
质量控制·实验室管理
出版日期:
2016-06-15

文章信息/Info

Title:
Investigation and Analysis of Reference Intervals and Decision Limits of Reticulocyte Count in China
作者:
章晓燕,王 薇,何法霖,钟 堃,王治国
北京医院 卫生部临床检验中心 北京市临床检验工程技术研究中心,北京 100730
Author(s):
ZHANG Xiao-yan,WANG Wei,HE Fa-lin,ZHONG Kun,WANGZhi-guo
National Center for Clinical Laboratories,Beijing Engineering Research Center of Laboratory Medicine,Beijing Hospital,Beijing 100730,China
关键词:
网织红细胞计数;  参考区间;  决定限
分类号:
R446.111
DOI:
10.3969/j.issn.1671-7414.2016.03.045
文献标志码:
A
摘要:
目的 调查目前中国网织红细胞计数参考区间的来源、上下限分布以及决定限的现状。方法 利用基于Web方式的室间质量评价软件系统,收集参加2014年网织红细胞计数参考区间调查的实验室参考区间上下限、临床决定水平、来源情况、方法原理、仪器和试剂等相关信息。按照仪器对各实验室进行分组,然后使用SPSS19.0软件对各组结果进行统计描述。结果 共276家实验室回报了有效结果。参考区间来源中所占比例依次为《全国临床检验操作规程》(53.99%)、仪器厂家说明书(16.30%)、教科书(9.78%)、试剂厂家说明书(9.06%)等。决定限来源中所占比例依次为《全国临床检验操作规程》(56.06%)、仪器厂家说明书(16.67%)、教科书(7.58%)、试剂厂家说明书(7.58%)等。不同组间的参考区间上下限以及临床决定水平的中位数与均值较为接近,但P2.5和P97.5差异较大。仅有45.65%和48.48%的实验室在使用参考区间和决定限前对其进行了验证。参考区间下限和决定限在不同组间无明显差异(P>0.05),但参考区间上限在不同仪器组间差异具有统计学意义(P<0.001)。结论 中国网织红细胞参考区间现状不尽如人意,应建立适应中国大多实验室和主要检测仪器的参考区间和决定限,为网织红细胞技术的标准化工作提供参考。
Abstract:
Objective To investigate and analyze the current situation of reference intervals and decision limits of reticulocyte count inChina.Methods The information related to upper/lower limits,decision limits,data resources,method principles,instruments and reagents of reticulocyte count in 2014 was collected via the external quality assessment software system based on website.The participants were divided according to their methods and instruments.The SPSS 19.0 was used for conducting the data analysis and statistics of results for each group.Results There were 276 laboratories submitting the valid results.The major resources for reference intervals came from national guide to clinical laboratory procedures(53.99%),instructions of instrument manufacturers(16.30%),textbook(9.78%),instructions of reagents manufacturers(9.06%)and others.The major resources for decision limits were national guide to clinical laboratory procedures(56.06%),instructions of instrument manufacturers(16.67%),textbook(7.58),instructions of reagents manufacturers(7.58%)and others.The medians and averages of upper/lower limits and decision limits among subgroups were close,whereasthe P2.5 and P97.5 were significant discrepancies.Only 45.65% and 48.48% of participant laboratories had verified reference intervals and decision limits before clinic use.The lower limits and decision limits among subgroups had nostatistical difference(P>0.05),where as the upper limits of different instrument groups had significant differences(P<0.001).Conclusion The current situation of reference intervals and decision limits of reticulocyte count is unsatisfactory.It is certain to establish auniformed reference interval and decision limits for reticulocyte count,which provides a reference for the standardization of reticulocyte count testing.

参考文献/References:

[1] 王治国.临床检验生物学变异与参考区间[M].北京:人民卫生出版社,2012:89-94.
[2] Fuentes-Arderiu X,Mas-Serra R,Alumà-Trullàs A,et al.Guideline for the production of multicentre physiological reference values using the same measurement system.A proposal of the Catalan Association for Clinical LaboratorySciences[J].Clin Chem Lab Med,2004,42(7):778-782.
[3] 程 俊.网织红细胞参数与红细胞参数在孕妇缺铁性贫血的临床应用研究[J].临床和实验医学杂志,2013,12(11):885-886,888. Cheng J.The diagnostic value of reticulocyte parameters and red blood cells in pregnant women in iron deflciency anemia[J].J Clin Exper Med,2013,12(11):885-886,888.
[4] Paul SH, Amadeo JP.Reference intervals:a user's guide[M].Washington DC:AACC press,2005:31-36.
[5] 费 阳,王 薇,钟 堃,等.235家临床实验室红细胞沉降率参考区间和医学决定水平的现况调查[J].检验医学与临床,2016,13(1):13-15. Fei Y,Wang W,Zhong K,et al.Investigation and analysis of reference intervals and medicine decide levels of erythrocyte sedimentation rate in 235clinical laboratories[J].Lab Med Clin,2016,13(1):13-15.
[6] 王 薇,钟 堃,何法霖,等.2012年全国凝血试验检验项目参考区间现状调查分析[J].中华血液学杂志,2015,36(1):39-43. Wang W,Zhong K,He FL,et al.The investigation of reference intervals application status of the analysis in coagulation test[J].Chin J Hematol,2015,36(1):39-43.
[7] Solberg HE.International Federation of Clinical Che-mistry(IFCC),Scientific committee,Clinical Section.Expert Panel on Theory of Reference Values,and International Committee for Standardization in Haematology(ICSH)Standing Committee on Reference Values.Approved recommendation(1986)on the theory of reference values.Part 1.The concept of reference values[J].J Clin Chim Acta,1987,25(5):337-342.
[8] 曾 洁,陈文祥,申子瑜.参考区间研究现状概述[J].中华检验医学杂志,2010,33(6):570-573. Zeng J,Chen WX,Shen ZY.Reference interval research overview[J].Chinese Journal of Laboratory Medicine,2010,33(6):570-573.
[9] 中华人民共和国卫生部.中华人民共和国卫生行业标准:临床实验室检验项目参考区间的制定WS/T 402-2012[S].北京:中国标准出版社,2013.
[10] 尚 红,陈文祥,潘柏申,等.中国成人常用肝功能和电解质及血细胞分析项目参考区间[J].中华检验医学杂志,2013,36(5):393-394. Shang H,Chen WX,Pan BS,et al.Reference intervals for common tests of liver function electrolytes and blood cell analysis of chinese adults[J].ChinJ Lab Med,2013,36(5):393-394.
[11] International Organization for Standardization 15 18 9.Medical laboratories.Requirements for quality and competence[S].Distributed Through AmericanNational Standards Institute,2012.

相似文献/References:

[1]钟 堃,王 薇,何法霖,等.国内临床化学9项常规项目参考区间与即将发布的卫生行业标准的比较和分析[J].现代检验医学杂志,2015,30(03):38.[doi:10.3969/j.issn.1671-7414.2015.03.011]
　ZHONG Kun,WANG Wei,HE Fa-lin,et al.Comparison and Analysis of the Reference Intervals of 9 Serum Routine Chemical Analytes between the Current Application of Clinical Laboratories and the Latest Health Industrial Standards Draft for Approval[J].Journal of Modern Laboratory Medicine,2015,30(03):38.[doi:10.3969/j.issn.1671-7414.2015.03.011]
[2]钟 堃,王 薇,何法霖,等.半胱氨酸蛋白酶抑制剂C和β2微球蛋白参考区间的调查与分析[J].现代检验医学杂志,2015,30(04):32.[doi:10.3969/j.issn.1671-7414.2015.04.008]
　ZHONG Kun,WANG Wei,HE Fa-lin,et al.Investigation and Analysis of Reference Intervals of Cystatin C and β2-Microglobulin[J].Journal of Modern Laboratory Medicine,2015,30(03):32.[doi:10.3969/j.issn.1671-7414.2015.04.008]
[3]钟 堃,王 薇,何法霖,等.全国231家临床实验室23项干化学 与湿化学检验项目参考区间的比较和分析[J].现代检验医学杂志,2015,30(01):145.[doi:10.3969/j.issn.1671-7414.2015.01.044]
　ZHONG Kun,WANG Wei,HE Fa-lin,et al.Comparison of the Reference Intervals of 23 Analytes between Wet Chemistry and Dry Chemistry in 231 Clinical Laboratories in China[J].Journal of Modern Laboratory Medicine,2015,30(03):145.[doi:10.3969/j.issn.1671-7414.2015.01.044]
[4]乔 军,毛华杰,白家瑞,等.西安地区同型半胱氨酸参考区间的初步建立[J].现代检验医学杂志,2016,31(05):130.[doi:10.3969/j.issn.1671-7414.2016.05.037]
　QIAO Jun,MAO Hua-jie,BAI Jia-rui,et al.Investigation on Serum Homocysteine for Reference Range in Xi'an[J].Journal of Modern Laboratory Medicine,2016,31(03):130.[doi:10.3969/j.issn.1671-7414.2016.05.037]
[5]王丽娜,葛君琍,郭苗.宝鸡地区成年健康人群血清胱抑素C参考区间调查[J].现代检验医学杂志,2016,31(06):141.[doi:10.3969/j.issn.1671-7414.2016.06.042]
　WANG Li-na,GE Jun-li,GUO Miao.Investigation of Reference Range of Serum Cystatin C of Healthy People in Baoji[J].Journal of Modern Laboratory Medicine,2016,31(03):141.[doi:10.3969/j.issn.1671-7414.2016.06.042]
[6]何金泉,吴彩明,李国强.广东虎门地区健康人群低血红蛋白密度(LHD%)参考区间的建立和临床应用[J].现代检验医学杂志,2017,32(02):86.[doi:10.3969/j.issn.1671-7414.2017.02.023]
　HE Jin-quan,WU Cai-ming,LI Guo-qiang.Building the Reference Range of Low Hemoglobin Density(LHD%) of Healthy Population in Humen District of Guangdong and Discuss Its Clinical Application Value[J].Journal of Modern Laboratory Medicine,2017,32(03):86.[doi:10.3969/j.issn.1671-7414.2017.02.023]
[7]卢美红,鞠少卿,丛 辉,等.南通地区健康人群血清NSE电化学发光法检测参考区间的调查[J].现代检验医学杂志,2018,33(04):59.[doi:10.3969/j.issn.1671-7414.2018.04.015]
　LU Mei-hong,JU Shao-qing,CONG Hui,et al.Investigate of Serum NSE Reference Range of Healthy People in Nantong with Electrochemiluminescence[J].Journal of Modern Laboratory Medicine,2018,33(03):59.[doi:10.3969/j.issn.1671-7414.2018.04.015]
[8]刘纯岳,林帝金,方俊粤,等.健康成年人血清α-L-岩藻糖苷酶参考区间的建立及适用性评价[J].现代检验医学杂志,2018,33(05):102.[doi:10.3969/j.issn.1671-7414.2018.05.027]
　LIU Chun-yue,LIN Di-jin,FANG Jun-yue,et al.Establishment and Applicability Evaluation of Reference Interval for Serum AFU of Healthy Adults[J].Journal of Modern Laboratory Medicine,2018,33(03):102.[doi:10.3969/j.issn.1671-7414.2018.05.027]
[9]肖光军,刘艳婷,杨 洪,等.四川省遂宁地区表观健康成年人群血浆FMN,FMN/TP比值及FMN/ALB比值参考区间的建立[J].现代检验医学杂志,2019,34(02):56.[doi:10.3969/j.issn.1671-7414.2019.02.015]
　XIAO Guang-jun,LIU Yan-ting,YANG Hong,et al.Establishment of Reference Interval for PlasmaFMN,FMN/TP and FMN/ALB Value among Apparently HealthyAdult Population in Suining of Sichuan[J].Journal of Modern Laboratory Medicine,2019,34(03):56.[doi:10.3969/j.issn.1671-7414.2019.02.015]
[10]张 勇,刘 婧,扈会整,等.陕西省咸阳地区正常人群尿液有形成分参考区间的建立[J].现代检验医学杂志,2019,34(03):112.[doi:10.3969/j.issn.1671-7414.2019.03.027]
　ZHANG Yong,LIU Jing,HU Hui-zheng,et al.Establishment of A Reference Interval for Urine Formationin Healthy People in Xianyang Region,Shaanxi Province[J].Journal of Modern Laboratory Medicine,2019,34(03):112.[doi:10.3969/j.issn.1671-7414.2019.03.027]

备注/Memo

备注/Memo:
基金项目:北京市自然科学基金资助项目(7143182),北京医院课题资助(BJ-2015-025)。 作者简介:章晓燕(1992-),女,在读研究生,研究方向:实验室管理学。 通讯作者:王治国,E-mail:zgwang@nccl.org.cn。
更新日期/Last Update: 2016-06-25