[1]陈 蕾,刘志忠,贾淑芬,等.双管单色荧光PCR法与基因芯片法检测CYP2C19基因多态性的比较研究[J].现代检验医学杂志,2016,31(04):51-53,57.[doi:10.3969/j.issn.16717-414.2016.04.013]
　CHEN Lei,LIU Zhi-zhong,JIA Shu-fen,et al.Comparing Study of Double Tube and One-Color Fluorescent PCR Technology and Gene Chip Method in Detecting the CYP2C19 Gene Polymorphisms[J].Journal of Modern Laboratory Medicine,2016,31(04):51-53,57.[doi:10.3969/j.issn.16717-414.2016.04.013]
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双管单色荧光PCR法与基因芯片法检测CYP2C19基因多态性的比较研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年04期
页码:
51-53,57
栏目:
论著
出版日期:
2016-08-10

文章信息/Info

Title:
Comparing Study of Double Tube and One-Color Fluorescent PCR Technology and Gene Chip Method in Detecting the CYP2C19 Gene Polymorphisms
文章编号:
1671-7414(2016)04-051-04
作者:
陈 蕾1; 刘志忠1; 贾淑芬1; 方 芳2; 康熙雄2
1.中国康复研究中心北京博爱医院检验科,北京 100068;
2.首都医科大学附属北京天坛医院实验诊断中心,北京 100050
Author(s):
CHEN Lei1; LIU Zhi-zhong1; JIA Shu-fen1; FANG Fang2; KANG Xi-xiong2
1.Department of Clinical Laboratory,Beijing Bo'ai Hospital,China Rehabilitation Research Center,Beijing 100068,China;
2.Exparimental Diagnosis Center,Affiliated Beijing Tiantan Hospital,Capital Medical University,Beijing 100050,China
关键词:
CYP2C19;  荧光PCR;  基因芯片;  多态性;  单核苷酸
分类号:
Q503; Q786
DOI:
10.3969/j.issn.16717-414.2016.04.013
文献标志码:
A
摘要:
目的 建立快速检测CYP2C19*2和CYP2C19*3 基因多态性的实验方法。方法 从样本库中选取100例血液样本。设计针对CYP2C19*2和CYP2C19*3基因多态性位点的特异性引物和taqman荧光探针,通过双管单色荧光PCR法检测基因型,与基因芯片法进行比较验证。结果 双管单色荧光PCR法能很好地鉴别CYP2C19*2和CYP2C19*3基因型,与基因芯片结果一致率为100%。结论 双管单色荧光PCR法能快速有效地进行CYP2C19*2和CYP2C19*3基因多态性分型,该方法期待用于临床实验室以指导个体化用药。
Abstract:
Objective To establish a rapid method for detection of CYP2C19*2 and CYP2C19*3 gene polymorphisms.Methods A total of 100 peripheral blood samples were collected from the sample library of the clinic laboratory.The specific primers and fluorescent probe sequences in the sites of CYP2C19*2 and CYP2C19*3 gene polymorphisms were designed,and the two gene polymorphisms were detected by double tube and one-color fluorescent PCR directly,and validated by a commercialgene chip method.Results The double tube and one-color fluorescent PCR technology couldidentify CYP2C19*2 and CYP2C19*3 single nucleotide polymorphisms,achieving 100% consistence with gene chip method.Conclusion The double tube and one-color fluorescent PCR technology based on the specific probe andprimer of CYP2C19*2 and CYP2C19*3 single nucleotide polymorphisms is effective,rapid and reliable in identifying genotypes.This method is expected to be applied in clinical laboratory to guide personalized medicine.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:首都医科大学科研基金项目(13JL36)。
作者简介:陈 蕾(1975-),女,硕士,主管技师,主要从事临床分子生物学检验工作,E-mail:amoon cl@163.com。
通讯作者:刘志忠(1970-),男,博士,E-mail:lzzlab@126.com。
更新日期/Last Update: 2016-08-10