[1]鲁晓燕a,刘曼华a,赵 枰b,等.紧密连接蛋白Claudin-4在子宫内膜异位症组织中的表达[J].现代检验医学杂志,2016,31(04):62-64.[doi:10.3969/j.issn.16717-414.2016.04.016]
 LU Xiao-yana,LIU Man-huaa,ZHAO Pingb,et al.Expressions of Claudin-4 mRNA in Endometriosis[J].Journal of Modern Laboratory Medicine,2016,31(04):62-64.[doi:10.3969/j.issn.16717-414.2016.04.016]
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紧密连接蛋白Claudin-4在子宫内膜异位症组织中的表达()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年04期
页码:
62-64
栏目:
论著
出版日期:
2016-08-10

文章信息/Info

Title:
Expressions of Claudin-4 mRNA in Endometriosis
文章编号:
1671-7414(2016)04-062-03
作者:
鲁晓燕a刘曼华a赵 枰b乔海风a刘颖蕾a朱 燕c
南通市第一人民医院a.妇产科;
b.检验科;
c.病理科,江苏南通 226001
Author(s):
LU Xiao-yanaLIU Man-huaaZHAO PingbQIAO Hai-fengaLIU Ying-leiaZHU Yanc
a.Gynaecology and Obstetrics;
b.Depatment of ClinicalLaboratory;
c.Department of Pathology,the First People's Hospital of Nantong,Jiangsu Nantong 226001,China
关键词:
子宫内膜异位症 紧密连接蛋白-4
分类号:
R711.74; R392.11
DOI:
10.3969/j.issn.16717-414.2016.04.016
文献标志码:
A
摘要:
目的 探讨紧密连接蛋白Claudin-4在子宫内膜异位症(内异症)在位、异位内膜与正常内膜中的表达,及其在内异症发病中的作用。方法采用RT-PCR的方法检测35例卵巢子宫内膜异位囊肿患者的在位、异位内膜组织中紧密连接蛋白Claudin-4 mRNA的表达,并与30例正常对照子宫内膜比较。结果 ①Claudin-4 mRNA在在位、异位及对照组织中表达量分别为12.38±6.12,4.59±1.35和13.05±6.48。异位内膜组织中Claudin-4 mRNA的表达量低于在位和对照组内膜组织,差异有统计学意义(P<0.01)。在位与对照内膜组织比较,Claudin-4mRNA的表达量差异无统计学意义(P>0.05)。②在异位内膜组织中,Ⅰ,Ⅱ期患者的Claudin-4 mRNA的表达量(6.74±1.92)较Ⅲ,Ⅳ期患者(3.82±1.01)明显升高,差异也有统计学意义(P<0.01)。③在位与对照内膜组织中,增生期Claudin-4 mRNA的表达量(14.01±7.02)较分泌期(11.14±5.48)明显升高,差异也有统计学意义(P<0.05); 异位组内膜组织中,增生期与分泌期Claudin-4 mRNA的表达量差异无统计学意义(P>0.05)。结论 Claudin-4在异位内膜中低表达可能与子宫内膜异位症的发生发展有关。
Abstract:
Objective To investigate mRNA expression of claudin-4 in the eutopic and ectopic endometrium of women with endometriosis and normal endometrium,and evaluate the role of clandin-4 in the pathogenesis of endometriosis.Methods The mRNA expression of claudin-4 ineutopic and ectopic endometrium of patients with endometriosis(n=35)andin normal endometrium from control group(n=30)were detected by reverse transcription-polymerase chain reaction.Results ①In eutopic and ectopic endometriam of women with endometriosis and control endometrium,the expression of claudin-4 mRNA was 12.38±6.12,4.59±1.35 and 13.05±6.48,respectively.Expression of claudin-4 mRNA was significantly lower in theectopic endometriotic tissue than in the eutopic endometrium and the control endometrium(P<0.01).No significant difference was found between eutopic endometrium from women with endometriosis and control endometrium(P>0.05).②Expression of claudin-4 mRNA levels of mild endometriosis were significantly higher than that of severe endometriosis in the ectopic endometriotic tissue(6.74±1.92 vs 3.82±1.01)(P<0.01).③In eutopic and control endometriam,Expression of claudin-4 mRNA levels were significantly higher in the proliferative phase than that in the secretory phase(14.01±7.02 vs 11.14±5.48)(P<0.05).In ectopic endometriam,no significant difference was found between proliferative phase and secretory phase(P>0.05).Conclusion Down-regulated expression of claudin-4 could be involved in the development of endometriosis lesions.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:2014南通市社会事业科技创新与示范(重点病种的临床规范化诊疗)(HS2014074),南通市卫生局青年基金资助项目(WQ2014023)。
作者简介:鲁晓燕(1971-),女,医学硕士,主任医师,研究方向:妇科内分泌,Tel:13962938293,E-mail:zhaopinghonghong@163.com。
更新日期/Last Update: 2016-08-10