[1]赵和平,王冀邯,蒋文艳,等.骨科医院多重耐药鲍曼不动杆菌耐药性与基因同源性分析[J].现代检验医学杂志,2016,31(05):88-90.[doi:10.3969/j.issn.1671-7414.2016.05.023]
　ZHAO He-ping,WANG Ji-han,JIANG Wen-yan,et al.Drug Resistance and Homology Analysis of Multi-drug Resistant Acinetobacter Baumannii in Orthopaedic Hospital[J].Journal of Modern Laboratory Medicine,2016,31(05):88-90.[doi:10.3969/j.issn.1671-7414.2016.05.023]
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骨科医院多重耐药鲍曼不动杆菌耐药性与基因同源性分析()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年05期
页码:
88-90
栏目:
论著
出版日期:
2016-10-21

文章信息/Info

Title:
Drug Resistance and Homology Analysis of Multi-drug Resistant Acinetobacter Baumannii in Orthopaedic Hospital
文章编号:
1671-7414(2016)05-088-03
作者:
赵和平; 王冀邯; 蒋文艳; 张 蓓; 赵 静; 于 燕
西安交通大学医学院附属红会医院检验科,西安 710054
Author(s):
ZHAO He-ping; WANG Ji-han; JIANG Wen-yan; ZHANG Bei; ZHAO Jing; YU Yan
Department of Clincal Laboratory, Honghui Hospital,College of Medicine of Xi'an Jiaotong University,Xi'an 710054,China
关键词:
多重耐药鲍曼不动杆菌;  耐药性;  基因外回文重复序列聚合酶链式反应;  同源性
分类号:
R378; Q786
DOI:
10.3969/j.issn.1671-7414.2016.05.023
文献标志码:
A
摘要:
目的 分析骨科医院多重耐药鲍曼不动杆菌(MDR-AB)的耐药情况及基因分型。方法 对2016年1月～7月临床检出的34株MDR-AB进行药敏分析,水煮法提取菌落DNA; 以基因外回文重复序列(repetitive extragenic palindromic,REP)为引物进行PCR扩增(REP-PCR),对凝胶电泳条带进行分型分析。结果 34株MDR-AB对17种抗生素中的15种药物耐药率在70%以上,对复方新诺明耐药率稍低为62%,对左旋氧氟沙星耐药率最低为15%。REP-PCR将34株MDR-AB分为Ⅰ,Ⅱ和Ⅲ三个基因型,其中Ⅰ型为主要的流行型别。结论 骨科医院MDR-AB感染情况严重,且主要由同一种基因型构成,临床应合理使用抗菌药物,定期监测耐药性变迁,避免院内传播。
Abstract:
Objective To investigate the drug resistance and homology status of Multi-drug Resistant Acinetobacter Baumannii(MDR-AB)in orthopaedic hospital.Methods 34 strains MDR-AB were isolated from 2016.1～2016.7 for DNA extraction and were typed by repetitiveextragenic palindromie-polyrnerase chain reaction(REP-PCR).Results The resistance rate of MDR-AB were ≥70% to 15 of 17 antimicrobials,except to cotrimoxazole(14%)and levofloxacin(61%),34 strains of MDR-ABwere divided into three types by REP-PCR including type Ⅰ,type Ⅱ and typeⅢ.Conclusion Drug resistance of MDR-AB was severe andmainly composed of the same genotype(type Ⅰ).Rational use of antimicrobial and regular monitoring of drug resistance is necessary to reduce the nosocomial transmission.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:西安市卫生局科技计划项目(J2014033)。
作者简介:赵和平(1967-),男,本科,副主任技师,从事临床医学检验工作,Tel:029-62818662,E-mail:redcrossjyk@163.com。
通讯作者:于 燕(1974-),女,硕士研究生,副主任技师,从事临床医学检验工作,Tel:029-62818662,E-mail:732867304@qq.com。
更新日期/Last Update: 2016-10-30