[1]邓顺江,黄韦华,吴林洪,等.miR-205在ITP患者外周血单个核细胞中的表达及意义[J].现代检验医学杂志,2016,31(06):10-12,16.[doi:10.3969/j.issn.1671-7414.2016.06.003]
　DENG Shun-jiang,HUANG Wei-hua,WU Lin-hong,et al.Expression of miR-205 in the Peripheral Blood Mononuclear Cells from Patients with Primary Immune Thrombocytopenia[J].Journal of Modern Laboratory Medicine,2016,31(06):10-12,16.[doi:10.3969/j.issn.1671-7414.2016.06.003]
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miR-205在ITP患者外周血单个核细胞中的表达及意义()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年06期
页码:
10-12,16
栏目:
论著
出版日期:
2016-12-20

文章信息/Info

Title:
Expression of miR-205 in the Peripheral Blood Mononuclear Cells from Patients with Primary Immune Thrombocytopenia
作者:
邓顺江1; 黄韦华1; 吴林洪2; 张蕾3; 叶辛1; 刘鹏1; 李腾达1; 龙曙萍1; 钱琤4; 邓安梅1
1.第二军医大学附属长海医院,上海 200433; 2.第二军医大学,上海200433; 3.第二军医大学附属长征医院,上海 200003; 4.解放军第100医院,江苏苏州 215007
Author(s):
DENG Shun-jiang1; HUANG Wei-hua1; WU Lin-hong2; ZHANG Lei3; YE Xin1;  LIU Peng1; LI Teng-da1; LONG Shu-ping1; QIAN Cheng4; DENG An-mei1
1.Changhai Hospital,the Second Militray Medical University,Shanghai 200433,China; 2.the Second Militray Medical University,Shanghai 200433,China; 3.Changzheng Hospital,the Second Militray Medical University,Shanghai 200003,China; 4.NO.100 Hospital of PLA,Jiangsu Suzhou 215007,China
关键词:
血小板减少;  外周血单个核细胞;  miR-205;  血小板计数
分类号:
R558.2; R392.11
DOI:
10.3969/j.issn.1671-7414.2016.06.003
文献标志码:
A
摘要:
目的 研究micro-RNA(miR-205)在原发性免疫性血小板减少症(primary immune thrombocytopenia,ITP)患者外周血单个核细胞(peripheral bloodmononuclear cells,PBMC)中的表达和意义。方法 收集43例ITP患者和38例健康人的外周静脉血2 ml,分离PBMC,提取细胞总RNA,逆转录合成cDNA,以U6为内参,应用实时荧光定量PCR(Real time PCR)的方法检测miR-205的表达量; 分析miR-205与血小板计数结果之间的相关性。结果 ITP初诊患者(Naive ITP)PBMC miR-205的表达水平与健康对照组相比上调(1.81±0.48 vs 0.92±0.25,t=10.31,P<0.01); 治疗后不完全缓解组(incmplete remissiongroup,IR group)和初诊患者相比,miR-205的表达水平差异无统计学意义(P=0.112),和正常对照组相比,则表达升高(1.63±0.65 vs 0.92±0.25,t=7.2,P<0.01); 14例治疗后完全缓解组(complete remission group,CR group)治疗后(After treatment)PBMC miR-205的表达低于治疗前(before treatment)的表达水平(1.07±0.43 vs 1.91±0.34,t=5.68,P<0.01); ITP患者PBMC中miR-205的表达量与血小板计数呈负相关(r=-0.0696,P<0.05)。结论 miR-205在ITP初诊患者PBMC中高表达,而治疗完全缓解后表达水平又降低,并且表达水平与血小板数量呈负相关,表明其可能与ITP的发生发展相关,或许会成为ITP诊疗新的标志物。
Abstract:
Objective To investigate the expression and clinical significance of microRNA(miR-205)in the peripheral blood mononuclear cells(PBMCs)of Primary Immune Thrombocytopenia(ITP)patients.MethodsThe real-time fluorescent quantitative PCR(real time PCR)was used to determine the expression of miR-205 in the peripheral blood of 43 ITP patients and 38 healthy controls.The correlations between the expression of miR-205 and platelet counts was analysed.Results The relativeexpression levels of miR-205 in health controls(0.92±0.25)were significantly lower than that in naive ITP(1.81±0.48)and incmplete remission group(1.63±0.65)(all P<0.01).The difference of miR-205 level between incmplete remission group and naive ITP group did not reach statistical significance(P=0.112).Expression of miR-205 after complete remission in peripheral bloodmononuclear cells(PBMC)was lower than its level at diagnosis expression of miR-205(1.07±0.43 vs 1.91±0.34,t=5.68,P<0.01).In addition.the miR-205 level in ITP patients was negativel correlated with the platelet counts(r=-0.0696,P<0.05).Conclusion The miR-205 expression in ITP patients was significantly increased,indicating miR-205 play an important role in the pathogenes of ITP,may becom a new potential diagnosis and treatment target of ITP.

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备注/Memo

备注/Memo:
基金项目:973计划(2013CB531606),国家自然科学基金(81471605,81401358,81501397,31500721,81501398,81302579,81273282,81202353),上海申康基金(SHDC22014014),上海科学教育基金(D14017),军队科研基金(BWS14J023,12MA056,5ZD009,15XD007),美捷登基金(MJR20150019)。 作者简介:邓顺江(1993-),男,本科,检验技师,E-mail:1114008320@qq.com。 黄韦华(1991-),女,在读硕士研究生,E-mail:huangweitina@163.com,共同第一作者。 通讯作者:钱 琤,主治医师,E-mail:qiancheng824@163.com。
更新日期/Last Update: 2016-12-20