[1]牛志立,张平安,童永清.探讨性别和年龄在慢性丙型肝炎患者 外周血Toll样受体3和7基因表达中的影响[J].现代检验医学杂志,2015,30(01):23-26.[doi:10.3969/j.issn.1671-7414.2015.01.007]
　NIU Zhi-li,ZHANG Ping-an,TONG Yong-qing.Exploring Impact of Gender and Age Discrepancies on the Expression of Toll-like Receptor 3 and 7 Gene mRNA of Patients with Chronic Hepatitis C Infection[J].Journal of Modern Laboratory Medicine,2015,30(01):23-26.[doi:10.3969/j.issn.1671-7414.2015.01.007]
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探讨性别和年龄在慢性丙型肝炎患者 外周血Toll样受体3和7基因表达中的影响()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年01期
页码:
23-26
栏目:
论著
出版日期:
2015-01-30

文章信息/Info

Title:
Exploring Impact of Gender and Age Discrepancies on the Expression of Toll-like Receptor 3 and 7 Gene mRNA of Patients with Chronic Hepatitis C Infection
文章编号:
1671-7414(2015)01-023-04
作者:
牛志立; 张平安; 童永清
武汉大学人民医院检验科,武汉 430060
Author(s):
NIU Zhi-li; ZHANG Ping-an; TONG Yong-qing
Department of Laboratory Medicine,Renmin Hospital of Wuhan University,Wuhan 430060,China
关键词:
慢性丙型肝炎;  外周血单个核细胞;  Toll样受体;  实时荧光定量PCR
分类号:
R512.63; Q786
DOI:
10.3969/j.issn.1671-7414.2015.01.007
文献标志码:
A
摘要:
目的 观察不同性别和年龄慢性丙型肝炎(CHC)患者外周血单个核细胞(PBMC)中Toll样受体3(TLR3)和Toll样受体7(TLR7)mRNA表达,探讨性别和年龄在CHC患者外周血TLR3和TLR7基因表达中的影响。方法 选择CHC患者115例,健康人群113例,取抗凝全血,实时荧光定量PCR检测TLR3和TLR7 mRNA表达。结果 CHC患者TLR3和TLR7表达量与健康人群相比,差异均有统计学意义(t=38.73,6.16,P<0.05)。CHC患者中非绝经期女性TLR3和TLR7表达量与绝经期女性和青年男性相比,差异均有统计学意义(t=61.210,6.464,P<0.05; t=24.166,26.266,P<0.05)。CHC患者中老年男性TLR3和TLR7表达量与青年男性相比,差异均有统计学意义(t=86.349,19.583,P<0.05),而与绝经期女性相比,仅TLR3表达量差异有统计学意义(t=122.941,P<0.05)。不同性别和年龄CHC患者的TLR3,TLR7基因表达量与HCV-RNA载量均无相关性(|r|<0.40,P>0.05),但是青年女性CHC患者HCV含量均比青年男性和老年女性低,差异有统计学意义(t=3.49,2.51,P<0.05),老年男性患者HCV含量比青年男性低,差异有统计学意义(t=2.35,P<0.05),老年男性和女性CHC患者HCV-RNA含量差异无统计学意义(t=1.20,P>0.05)。结论 性别和年龄与CHC患者TLR的表达有一定的关系,为临床合理有效治疗CHC提供理论依据和新方法。
Abstract:
Objective Investigating the expression of TLR3and TLR7 mRNA in peripheral blood mononuclear cells(PBMC)of patients with chronic hepatitis C infection(CHC),to explore the effects of gender and age on Toll-like receptor expression.Methods Peripheral blood wascollected from 115 patients of chronic hepatitis C infection,113 healthy individuals.Expression levels of TLR3 and TLR7 mRNA were detected by real-time quantitative PCR.Results The expression of TLR3 and TLR7 mRNA were significant difference between patients with CHC infection and healthy individuals(t=38.73,6.16,P<0.05),respectively.The expression of TLR3and TLR7 mRNA were significant difference between premenopausal females with CHC infection,postmenopausal females and young males of CHC infection(t=61.210,6.464,P<0.05; t=24.166,26.266,P<0.05),respectively.The expression of TLR3 and TLR7 mRNA were significant differences between old males andyoung males of chronic hepatitis CHC infection(t=86.349,19.583,P<0.05).The expression of TLR3 mRNA was significant differences between old males andpostmenopausal females of CHC infection(t=122.941,P<0.05).There was no correlation between the expressions of TLR3 and TLR7 and HCV-RNA load of CHCpatents of Gender and age discrepancies(|r|<0.40,P>0.05).The HCV-RNA load of premenopausal females was significant lower than young males and old females(t=3.49,2.51,P<0.05),the load of old males was lower thanold females(t=2.35,P<0.05),however,there was no significant differences between old males and old females(t=1.20,P<0.05).Conclusion Gender and age discrepancies have a relationship with the expression of Toll-like receptors of patients with CHC infection,which may provide atheoretical basis and a new method for CHC.

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备注/Memo

备注/Memo:

作者简介:牛志立(1989-),男,在读硕士,从事临床免疫学研究,Tel:18207156885,E-mail:1060128042@qq.com。
通讯作者:张平安,E-mail:zhangpingan@aliyun.com。
更新日期/Last Update: 2015-01-30