[1]许宏涛,陈东科,赖惠英.革兰阳性球菌对利奈唑胺耐药机制的研究[J].现代检验医学杂志,2016,31(06):20-22，27.[doi:10.3969/j.issn.1671-7414.2016.06.006]
　XU Hong-tao,CHEN Dong-ke,LAI Hui-ying.Study on the Linezolid Resistance Mechanism of Gram Positive Cocci[J].Journal of Modern Laboratory Medicine,2016,31(06):20-22，27.[doi:10.3969/j.issn.1671-7414.2016.06.006]
点击复制

革兰阳性球菌对利奈唑胺耐药机制的研究()
分享到：

《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年06期
页码:
20-22，27
栏目:
论著
出版日期:
2016-12-20

文章信息/Info

Title:
Study on the Linezolid Resistance Mechanism of Gram Positive Cocci
作者:
许宏涛; 陈东科; 赖惠英
北京医院检验科 国家老年医学研究中心,北京 100730
Author(s):
XU Hong-tao; CHEN Dong-ke; LAI Hui-ying
Department of Laboratory Medicine, Beijing Hospital,National Geriatric Research Centre,Beijing 100730,China
关键词:
利奈唑胺耐药;  革兰阳性球菌;  耐药机制
分类号:
R378.1; R446.5
DOI:
10.3969/j.issn.1671-7414.2016.06.006
文献标志码:
A
摘要:
目的 对利奈唑胺耐药革兰阳性球菌主要耐药分子机制进行初步研究。方法 采用KB法及E-test法对6株利奈唑胺耐药革兰阳性球菌进行药敏试验,并应用脉冲场凝胶电泳(PFGE),PCR及测序技术对菌株主要分子流行病学及耐药分子机制进行研究。结果 5株柯氏葡萄球菌PFGE分为2型,对利奈唑胺MIC介于16～64 mg/L,菌株均呈现cfr基因阳性、L3存在双位点突变; 1株粪肠球菌对利奈唑胺MIC为8 mg/L,耐药与23SrRNA存在G2576T点突变相关。结论 利奈唑胺耐药革兰阳性球菌在临床的出现应引起广泛关注,为有效进行抗感染治疗应重视对利奈唑胺耐药菌株的临床监测。
Abstract:
Objective To study the molecular resistance mechanism of linezolid resistant gram positive cocci.Methods KB and E-test methods were used in antibiotic susceptibility tests.PFGE,PCRand sequencing techniques were applied to study the molecular epidemiological characteristics and resistance mechnisms of linezolid to gram positive cocci.Results The MICs of linezolid of the 5 Staphylococcus cohnii isolates were from 16 to 64 mg/L,and they were all cfr gene positive and with L3 mutations,and there were two PFGE types.The linezolid MIC of one Enterococcus faecalis was 8 mg/L with G2576T mutation in 23SrRNA.Conclusion There were linezolid resistant gram positive cocci appeared inclinical samples,for the effective anti-infection treatment monitoring linezolid resistant gram positive cocci in clinical lab is important.

参考文献/References:

[1] Tsiodras S,Gold HS,Sakoulas G,et al.Linezolid resistance in aclinical isolate of Staphylococcus aureus[J].Lancet,2001,358(9277):207-208.
[2] Mendes RE,Deshpande LM,Farrell DJ,et al.Assessment of linezolid resistance mechanisms among Staphylococcus epidermidis causing bacteraemia in Rome,Italy[J].The Journal of Antimicrobial Chemotherapy,2010,65(11):2329-2335.
[3] Toh SM,Xiong L,Arias CA,et al.Acquisition of a natural resistance gene renders a clinical strain of methicillin-resistant Staphylococcus aureusresistant to the synthetic antibiotic linezolid[J].Molecular Microbiology,2007,64(6):1506-1514.
[4] Kehrenberg C,Schwarz S.Distribution of florfenicol resistance genes fexA and cfr among chloramphenicol-resistant Staphylococcus isolates[J].Antimicrobial Agents and Chemotherapy,2006,50(4):1156-1163. [1] Neu N, Duchon J, Zachariah P. TORCH infections[J].Clin Perinatol,2015,42(1):77-103.
[2] 张巧安.孕前及孕期妇女TORCH感染状况调查分析[J].数理医药学杂志,2015,28(11):1662-1663. Zhang QA.The investigation and analysis of infection status of pre-pregnancy and pregnant women TORCH[J].Journal of Mathematical Medicine,2015,28(11):1662-1663.
[3] 吴佳丽,朱建昌.365例妊娠期妇女TORCH感染的血清学检测及妊娠结局分析[J].中国微生态学杂志,2015,27(10):1218-1220. Wu JL,Zhu JC.Serological detection of TORCH in 365 pregnant womenand pregnancy outcome analysis[J].Chinese Journal of Microecology,2015,27(10):1218-1220.
[4] 符青松,蒋 英.深圳市龙岗地区孕妇TORCH感染情况分析[J].中国医学工程,2015(10):27-28. Fu QS,Jiang Y.Analysis of TORCH infection in pregnant women in Longgang District of Shenzhen City[J].Chinese Medical Engineering,2015(10):27-28.
[5] 李东明,张 硕,陶春凤,等.孕妇TORCH感染情况与不良妊娠结局的相关性[J].中国妇幼保健,2015,30(28):4788-4789. Li DM,Zhang S,Tao CF,et al.Correlation between TORCH infection in pregnant women and adverse pregnancy outcome[J].Maternal and Child Health Care of China,2015,30(28):4788-4789.
[6] 邓 刚,张平安,吴 薇.2 760例育龄妇女TORCH监测数据分析[J].公共卫生与预防医学,2015,26(4):116-118. Deng G,Zhang PA,Wu W.Analysis of TORCH monitoring data of 2760 cases of women of childbearing age[J].Journal of Public Health and Preventive Medicine,2015,26(4):116-118.
[7] 谢小娟,李小侠,李芒会,等.西安地区9338例孕妇TORCH检测结果分析[J].医学综述,2015,21(16):3016-3018. Xie XJ,Li XX,Li MH,et al.Analysis of 9338 cases of TORCH detection in pregnant women in Xi'an area[J].Medical Recapitulate,2015,21(16):3016-3018.
[8] 黄勤洲,成 艳,张娟玲,等.5 447例早中期孕妇TORCH检测结果分析[J].中国优生与遗传杂志,2015,23(4):82-83. Huang QZ,Cheng Y,Zhang JL,et al.Detection of TORCH in esrly pregnant women from 5447 cases[J].Chinese Journal of Birth Health and Heredity,2015,23(4):82-83.
[9] 韦珍珍,郑宗富,施水兰,等.对福州地区4 512例孕妇血清TORCH抗体检测结果的分析[J].当代医药论丛,2015,13(6):67. Wei ZZ,Zheng ZF,Shi SL,et al.Analysis of serum TORCH antibody in4512 cases of pregnant women in Fuzhou[J].Contemporary Medicine Forum,2015,13(6):67.
[10] 丁 睿,张智贤,曾 华,等.6 027例孕产妇及新生儿TORCH检测结果分析[J].国际检验医学杂志,2015,36(4):485-486. Ding R,Zhang ZX,Zeng H,et al.Analysis on TO-RCH tests in 6 027 casesof pregnant women,puerperae and neonates[J].International Journal of Laboratory Medicine,2015,36(4):485-486.
[11] 薛爱国,丁 伟,孙 霞,等.青岛地区1 694例妊娠早期孕妇TORCH感染状况的调查分析[J].中国优生与遗传杂志,2014(10):80-81. Xue AG,Ding W,Sun X,et al.Investigation and analysis of TORCH infection in 1694 cases of early pregnant women in Qingdao area[J].Chinese Journal ofBirth Health and Heredity,2014(10):80-81.
[12] 徐雪琴.温州地区2 061例孕妇TORCH感染的调查分析[C].第九届全国免疫学学术大会论文集,2014:258. Xu XQ.Investigation and analysis of TORCH infection in 2061 pregnant women in Wenzhou[C].Proceedings of the Ninth National Symposium on Immunology,2014:258.
[13] 韦平宣.532例孕妇唐氏综合征筛查及TORCH筛查分析[J].右江民族医学院学报,2014,36(4):600-601. Wei PX.Screening of Down's syndrome in 532 cases of pregnant women and the analysis of TORCH screening[J].Journal of Youjiang Medical University forNationalities,2014,36(4):600-601.
[14] 冯先华,郗 娟,蒋 冰,等.武汉地区3 072例孕妇TORCH检测结果分析[J].中国优生与遗传杂志,2014,22(7):88,93. Feng XH,Xi J,Jiang B,et al.Analysis of TORCH test results about 3072 cases of pregnant women inWuhan area[J].Chinese Journal of Birth Health and Heredity,2014,22(7):88-93.
[15] 高文静,乔 杰,莫顺华,等.东莞市城区孕妇TO-RCH感染情况调查分析[J].中国民族民间医药,2014(9):106-107. Gao WJ,Qiao J,Mo SH,et al.Dongguan city pregnant women TORCH investigation of infect[J].Chinese Journal of Ethnomedicine and Ethnopharmacy,2014(9):106-107.
[16] 米 东,祁 佩,陈淑琴,等.天津地区不孕妇女和孕妇TORCH感染的检测分析[J].国际检验医学杂志,2014,35(2):241-243. Mi D,Qi P,Chen SQ,et al.Detection and analysis of TORCH infection in infertile women and pregnant women in Tianjin[J].International Journal of Laboratory Medicine,2014,35(2):241-243.
[17] 靳庆娥,谷俊朝,苏建荣.2015年北京地区孕前妇女血清TORCH抗体及TNF-α水平检测与相关分析[J].现代检验医学杂志,2016,31(3):105-107. Jin QE,Gu JC,Su JR.Seroprevalence of TORCH infection within pre-pregnancy women and the relationship with TNF-α level during 2015 in Beijing area[J].Journal of Modern Laboratory Medicine,2016,31(3):105-107.
[18] 余 杰,王海萍,伏春明.上海虹口区孕前妇女TORCH三种病原体感染情况及与妊娠结局的关系分析[J].现代检验医学杂志,2014,29(5):127-129. Yu J,Wang HP,Fu CM.Study on TORCH infection status of pre-pregnant women and their relationship with pregnancy outcome in Shanghai Hongkou district[J].Journal of Modern Laboratory Medicine,2014,29(5):127-129.
[19] 黄 霁,杨 敏,吴成亮.九江地区孕龄妇女TO-RCH感染情况分析[J].当代医学,2015,21(30):162-163. Huang Q,Yang M,Wu CL.Analysis of TORCH infection in pregnant women in Jiujiang area[J].Contemporary Medicine,2015,21(30):162-163.

备注/Memo

备注/Memo:
作者简介:许宏涛(1973-),女,医学博士,主任技师,从事微生物和分子生物学工作,研究方向:分子生物学技术应用于病原微生物的检出及抗生素相关耐药机制研究,Tel,010-85133617,E-mail:taotao200703@sina.com。
更新日期/Last Update: 2016-12-20