[1]姚创利,赵 佳,鲁旭娟,等.冠心病患者血清脂蛋白相关磷脂酶A2水平与锰超氧化物歧化酶9 Ala/Val基因多态性关系探讨[J].现代检验医学杂志,2017,32(01):26-29.[doi:10.3969/j.issn.1671-7414.2017.01.008]
 YAO Chuang-li,ZHAO Ja,LU Xu-juan,et al.Study on the Relationship between Lipoprotein Associated Phospholipase A2 Level and Manganese Superoxide Dismutase 9 Ala/Val Genetic Polymorphism in Patients with Coronary Heart Disease[J].Journal of Modern Laboratory Medicine,2017,32(01):26-29.[doi:10.3969/j.issn.1671-7414.2017.01.008]
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冠心病患者血清脂蛋白相关磷脂酶A2水平与锰超氧化物歧化酶9 Ala/Val基因多态性关系探讨()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年01期
页码:
26-29
栏目:
论著
出版日期:
2017-01-25

文章信息/Info

Title:
Study on the Relationship between Lipoprotein Associated Phospholipase A2 Level and Manganese Superoxide Dismutase 9 Ala/Val Genetic Polymorphism in Patients with Coronary Heart Disease
文章编号:
1671-7414(2017)01-026-04
作者:
姚创利赵 佳鲁旭娟姜小建
西安市中心医院检验科,西安 710003
Author(s):
YAO Chuang-liZHAO JaLU Xu-juanJIANG Xiao-jian
Department of Clinical Laboratory,Xi'an Central Hospital,Xi'an 710003,China
关键词:
脂蛋白相关磷脂酶A2 锰超氧化物歧化酶 基因多态性 冠心病
分类号:
R541.4; R392.11
DOI:
10.3969/j.issn.1671-7414.2017.01.008
文献标志码:
A
摘要:
目的 探讨锰超氧化物歧化酶(Mn-SOD)基因多态性与脂蛋白磷脂酶A2(Lp-PLA2)联合检测在冠心病(CHD)早期诊断中的临床价值。方法选取冠心病患者组92例与健康对照组78例,分别检测各组血清中Lp-PLA2活性,Mn-SOD活性以及Mn-SOD基因多态性,分析血清Mn-SOD,Lp-PLA2及Mn-SOD基因多态性与CHD的相关性。测定项目所用方法:Mn-SOD采用比色法,Lp-PLA2采用连续监测法,应用测序法检测Mn-SOD 9 Ala/Val基因多态性的基因型。结果 CHD组Mn-SOD 9 VV基因型患者血清Lp-PLA2活性显著高于AV+AA基因型患者,差异具有统计学意义(P<0.01); VV基因型患者血清Mn-SOD活性显著低于AV+AA基因型患者,差异具有统计学意义(P<0.01)。结论 联合检测Mn-SOD 9 Ala/Val基因多态性和血清Lp-PLA2活性,可作为CHD早期诊断、预测的重要依据。
Abstract:
Objective To investigate the clinical value ofthe combined detection of manganese superoxide dismutase(Mn-SOD)genetic polymorphism and lipoprotein phospholipase A2(Lp-PLA2)in the early diagnosis of coronary heart disease(CHD).Methods 92 cases of coronaryheart disease patients and 78 cases of healthy control group were selected.Theactivity of Lp-PLA2,the activity of Mn-SOD and genotype of Mn-SOD 9 Ala/Valgenetic polymorphism were detected in the serum of each group via the use of colorimetry,continuous monitoring technique and gene-sequencing method respectively and then the correlation of serum Mn-SOD,Lp-PLA2 and Mn-SOD genetic polymorphism with CHD were analyzed.Results The Lp-PLA2 activity in serum of CHD groups with Mn-SOD 9 VV genotype was statistically significantly higher than that of patients with the AV+AA genotype(P<0.01).Theserum Mn-SOD activity in patients with VV genotype was significantlylower than that of those with AV+AA genotype(P<0.01).Conclusion Combined detection of Mn-SOD 9 Ala/Val genetic polymorphism and Lp-PLA2 activityin the serum can provide an important foundation for thediagnosis and prediction of coronary heart disease.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:陕西省科学技术研究发展计划项目(项目编号:2013K12-02-10)。
作者简介:姚创利(1962-),男,本科,副主任检验师,主要从事临床生物化学检验工作,E-mail:yaocl7270@163.com。
通讯作者:鲁旭娟(1966-),女,本科,主管检验师,E-mail:1323878654@qq.com。
更新日期/Last Update: 2017-01-20