[1]李桂荣,赵清侠a,宋 斌b,等.miR-221与miR-222在甲状腺乳头状癌组织中的表达及临床意义[J].现代检验医学杂志,2017,32(01):41-44.[doi:10.3969/j.issn.1671-7414.2017.01.012]
　LI Gui-rong,ZHAO Qing-xiaa,SONG Binb,et al.Expression of miR-221 and miR-222 in Papillary Thyroid Carcinoma Tissues and Its Clinical Significance[J].Journal of Modern Laboratory Medicine,2017,32(01):41-44.[doi:10.3969/j.issn.1671-7414.2017.01.012]
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miR-221与miR-222在甲状腺乳头状癌组织中的表达及临床意义()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年01期
页码:
41-44
栏目:
论著
出版日期:
2017-01-25

文章信息/Info

Title:
Expression of miR-221 and miR-222 in Papillary Thyroid Carcinoma Tissues and Its Clinical Significance
文章编号:
1671-7414(2017)01-041-04
作者:
李桂荣1; 赵清侠2a; 宋 斌2b; 刘 捷2c; 张 瑾2c; 李世东2c;  韩想利2c; 刘 晖2c; 张 文2c
1.陕西省第四人民医院耳鼻咽喉头颈外科,西安 710043;
2.陕西省人民医院a.手术二部;
b.普外二科;
c.耳鼻咽喉头颈外科,西安 710068
Author(s):
LI Gui-rong1; ZHAO Qing-xia2a; SONG Bin2b; LIU Jie2c;  ZHANG Jin2c; LI Shi-dong2c; HAN Xiang-li2c; LIU Hui2c; ZHANG wen2c
1.Department of ENT &
HNS,the Forth People's Hospital of Shaanxi,Xi'an 710043,China;
2a.the 2nd Department of OR;
2b.the 2nd Department of General Surgery;
2c.Department of ENT &
HNS,Shaanxi Provincial People's Hospital,Xi'an 710068,
关键词:
甲状腺乳头状癌;  微小核糖核酸221;  微小核糖核酸222;  病理学
分类号:
R736.1; R730.43
DOI:
10.3969/j.issn.1671-7414.2017.01.012
文献标志码:
A
摘要:
目的 探讨miR-221与miR-222在甲状腺乳头状癌(PTC)组织中的表达及其与临床病理因素之间的关系。方法 收集陕西省人民医院耳鼻咽喉头颈外科2015年5月～2016年5月就诊的甲状腺乳头状癌(43例)患者、结节性甲状腺肿(21例)及癌旁正常甲状腺组织(14例)共78例,行real time-PCR检测组织中的miR-221及miR-222,并结合临床病理因素进行分析,对结果进行统计学分析。结果甲状腺乳头状癌组中的miR-221及miR-222相对表达水平(11.54±3.37,10.67±2.45)高于结节性甲状腺肿组(3.21±1.12,2.89±1.23)及正常对照组(2.02±0.76,1.98±0.34),差异有统计学意义(t=3.62,3.25; 3.27,3.01,均P＜0.05),且与患者的性别、年龄、肿瘤大小等因素无关(P＞0.05),而与局部淋巴结转移、肿瘤侵犯包膜、远处转移以及临床分期相关(P＜0.05)。结节性甲状腺肿组中的miR-221及miR-222与正常对照组比较差异无统计学意义(t=0.91,0.79,P＞0.05)。结论miR-221及miR-222可作为甲状腺乳头状癌相对特异的分子标志物,对甲状腺乳头状癌的临床诊断及治疗有重要作用。
Abstract:
Objective To explore the expressions of miR-221 and miR-222 in papillary thyroid carcinoma(PTC),and relativities with clinical pathological features.Methods Samples from patientsof PCT(43 cases),nodular golter(21 cases),and para-carcinoma thyroid tissues(14 cases),78 cases in total(from 06/2015 to 05/2016,Shaanxi Provincial People's Hospital)were collected.Real time-PCR tests were carried out,then analyzed in relation to clinical pathology features,and statistical analysis was used to evaluate the results.Results The expressions of miR-221 and miR-222 were significantly higher in PTC(11.54±3.37,10.67±2.45)than in nodular golter(3.21±1.12,2.89±1.23)and normal thyroid tissue(2.02±0.76,1.98±0.34)(t=3.62,3.25; 3.27,3.01,all P＜0.05),which were significantly related with Regional lymph node metastasis,tumor invasion coated,distant metastasis and clinical stage(P＜0.05),while not related with the gender,age,or size of the tumor of the patients(P＞0.05),and no differences were found in nodular golter and in normal thyroid tissue(t=0.91,0.79,P＞0.05).Conclusion miR-221 and miR-222 could be considered as a specific molecular marker of PTC,may play an important role in thediagnosis and treatment on PTC.

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备注/Memo

备注/Memo:
基金项目:陕西省科学技术研究发展计划项目(2015SF050)。
作者简介:李桂荣(1974-),女,本科,副主任医师,主要研究方向:头颈肿瘤及鼻内镜技术,E-mail:526286281@qq.com。
通讯作者:张 文(1975-),女,在读博士,主任医师,主要研究方向:中耳炎、耳聋及头颈肿瘤,E-mail:smileww@foxmail.com。
更新日期/Last Update: 2017-01-20