[1]肖小平,郭 玲a,张 熊b.胆管癌组织LOXL2 mRNA与Tenascinm-C mRNA表达的临床应用研究[J].现代检验医学杂志,2017,32(03):79-81.[doi:10.3969/j.issn.1671-7414.2017.03.021]
　XIAO Xiao-ping,GUO Linga,ZHANG Xiongb.Clinical Application of the Expression of LOXL2 mRNA and Tenascin-C mRNA in Tissues of the Bile Duct Cancer[J].Journal of Modern Laboratory Medicine,2017,32(03):79-81.[doi:10.3969/j.issn.1671-7414.2017.03.021]
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胆管癌组织LOXL2 mRNA与Tenascinm-C mRNA表达的临床应用研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年03期
页码:
79-81
栏目:
论著
出版日期:
2017-05-25

文章信息/Info

Title:
Clinical Application of the Expression of LOXL2 mRNA and Tenascin-C mRNA in Tissues of the Bile Duct Cancer
文章编号:
1671-7 414(2017)03-079-03
作者:
肖小平1; 郭 玲2a; 张 熊2b
1. 西安市第八医院检验科，西安 710061；
2.咸阳市第一人民医院 a.病理科；
b. 检验科，陕西咸阳 712000
Author(s):
XIAO Xiao-ping1; GUO Ling2a; ZHANG Xiong2b
1.Department of Chinical Laboratory，the Eighth Hospital of Xi ’an City，Xi’an 710061，China；
2a.Department of Pathology；
2b.Department of Clinical Laboratory ， the First People’s Hospital of Xianyang City，Shaanxi Xianyang 712000，Ch ina
关键词:
胆管癌; 赖氨酰氧化酶样蛋白2 mRNA; Tenascin-C mRNA
分类号:
R735.8；R730.43
DOI:
10.3969/j.issn.1671-7414.2017.03.021
文献标志码:
A
摘要:
目的 探讨组织赖氨酰氧化酶样蛋白-2(LOXL2)mRNA和Tena scinm-C mRNA表达在胆管癌疾病中的临床应用价值。方法 收集35 例胆管癌患者(胆管癌组)和28例正常胆管组织(对照组)的血清及临床资料，采用实时荧光定 量PCR(real-time-PCR，RT-PCR)技术检测组织LOXL2 mRNA与Tenascin-C mRNA的表达， 探讨两标志物的变化与胆管癌疾病之间的关系。结果 组织LOXL2 mR NA和Tenascin-C mRNA在胆管癌组中的表达量分别为1.27±0.18，1.39±0.19；在对照 组的表达量分别为0.20±0.06，0.23±0.06。胆管癌组组织LOXL2 mRNA与Tenascin-C mRNA的表达与对照组比较显著增高，差异均有统计学意义(t=52.18，56.87，P均<0 .01)。胆管癌组两标志物的表达呈正相关性(r=0.687，P<0.01)。结论 组织LOXL2mRNA与Tenascin-CmRNA的表达可能成为胆管癌疾病的早期诊断及判断 其病程进展的分子标志物。
Abstract:
Objective To explore the clinical application o f the expression of LOXL2 mRNA and Tenascin-C mRNA in tissues for the disease w it h the bile duct cancer．Methods The serum and clinical da ta in 35 cases of patients with the bile duct cancer (cancer group) and 28 case s of patients with normal bile duct tissue (control group) were collected，used the real-time fluorescent quantitative PCR (real-time-PCR，RT-PCR) technolo gy to detect the expression of LOXL2 mRNA and Tenascin-C mRNA in tissues toobse rve the relationship between the changes and the bile duct cancer for the two ma rkers．Results The expression of LOXL2 mRNA and Tenascin -C mRNA in tissues in the cancer group were 1.27±0.18 and 1.39±0.19，whic h of ones in the control group were 0.20±0.06 and 0.23±0.06．In the cancer group，the expression of LOXL2 mRNA and Tenascin-C mRNA in tissues respectivel y wit h comparision to those in the control group were significantly higher，the diffe rences had statistical significance(t=52.18，56.87，P<0.01)，which of one s in the cancer group was positively related (r=0.687，P<0.01)．Conclution The expression of LOXL2 mRNA and Tenascin-C mRNA in ti ssues may be a molecular targets for the disease with the bile duct cancer in th e early diagnosis and judgment of progression in the courses of this disease．

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备注/Memo

备注/Memo:

作者简介：肖小平(1968-)，男，本科学历，主管检验师，研究方向：肿瘤 标志物的检测，E-mail：418079040@qq.com。
通讯作者：郭 玲(1979-)，女，本科学历，主治医师，研究方向 ：肝胆肿瘤疾病的诊断，E-mail：407205950@qq.com。
更新日期/Last Update: 2017-06-25