[1]王克迪,徐东江,苏建荣.血浆置换法和公式校正法纠正乳糜血对仪器法测定血红蛋白影响的探讨[J].现代检验医学杂志,2017,32(03):137-139,143.[doi:10.3969/j.issn.1671-7414.2017.03.038]
 WANG Ke-di,XU Dong-jiang,SU Jian-rong.Correction of the Impact of Chylemia on Hematology Analysis by Plasma Exchange and Formula Calibration Method[J].Journal of Modern Laboratory Medicine,2017,32(03):137-139,143.[doi:10.3969/j.issn.1671-7414.2017.03.038]
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血浆置换法和公式校正法纠正乳糜血对仪器法测定血红蛋白影响的探讨()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年03期
页码:
137-139,143
栏目:
研究简报·实验技术
出版日期:
2017-05-25

文章信息/Info

Title:
Correction of the Impact of Chylemia on Hematology Analysis by Plasma Exchange and Formula Calibration Method
文章编号:
1671-7414(2017)03- 137-04
作者:
王克迪1徐东江2苏建荣1
1.首都医科大学附属北京友谊医院 临床检验中心,北京 100050;
2.北京积水潭医院检验科,北京 100035
Author(s):
WANG Ke-di1XU Dong-jiang2SU Jian-rong1
1.Clinical Laboratory Center, Beijing Friendship Hospital,Capital Medical University,Beijing 100050,Ch ina;
2.Department of Clinical Laboratory,Beijing Jishuitan Hospital,Beijing 1 00035,China
关键词:
乳糜血血细胞检测血浆置换公式
分类号:
R446
DOI:
10.3969/j.issn.1671-7414.2017.03.038
文献标志码:
A
摘要:
目的 探讨乳糜血血浆置换法和公式校正法两种方法对乳糜 血血细胞检验结果的校正效果,为实验室能够准确提供血细胞检测结果提供依据。方法 收集10例无溶血、黄疸、乳糜的标本进行血细胞测定设为对照组,每个 标本分为4份,分别加入5,10,20和40 μl脂肪乳,制备不同程度的乳糜血,采用血浆置换 法处理标本,每份标本置换2次,然后用血细胞分析仪重新测定,将结果与对照组结果进行 比较。此外,将测定的乳糜血标本结果和离心后乳糜血浆的血细胞结果带入校正公式HGB 修正值=HGB修正前-(HGB乳糜血血浆-HGB乳糜血血浆×HCT修正 前),得出的HGB修正值与对照组和血浆置换法结果进行比较和统计学分析。结果 乳糜血可造成HGB,MCH和MCHC三项指标明显升高,与其真实值差异 有统计学显著性意义(P<0.05),经过2次血浆置换后该3项参数恢复到正常和稳定的状 态。公式校正法结果与真实值差异无统计学意义(P>0.05)。结论 乳糜血标本经血浆置换法和公式校正法处理后均可消除乳糜微粒对仪器法测定HGB的影响 ,为临床提供准确的结果。
Abstract:
Objective Comparison the corrective effect of p lasma exchange method and formula method for chylemia on hematology analysis.〖 WT5”HZ〗Methods A total of 10 samples without hemolysis,jaundice a nd lipemia were set as control group,each sample was divided into 4 parts,then 5,10,20 and 40 μl lipid emulsion was added.Each chylous samples was treated by plasma exchange method for two times,and routine blood test was reanalyzed with hematology analyzer.The comparisons before and after the exchange was made ,while the test results on each exchange were analyzed.For another part,the H GB of chylous plasma was tested,the value was substituted into HGB correcte d value= HGBbefore correction-(HGB chylous plasma-HGBchylous plasma×HCTbefore correction).Results The chyle mia could lead significant increase of HGB,MCH and MCHC (P<0.05).After pl asma exchanging for two times,the three parameters returned to normal and the c ount of WBC,RBC and PLT decline slightly with no significance.There were no d ifferences between plasma exchange method and formula calibration method.〖WT5 ”HZ〗Conclusion Plasma exchange method and formula calibration meth od could significantly reduce the impact of chylemia on routine blood analysis, which facilitate the clinical work with correct analysis of routine blood test.

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备注/Memo

备注/Memo:

作者简介:王克迪(1985-),女,硕士研究生,主治医师,研究方向: 临床检验诊断学,E-mail:kediv@163.com。
更新日期/Last Update: 2017-06-25