[1]李 洪,唐 浏,郑 淋,等.结肠癌组织中microRNA-126表达及临床意义[J].现代检验医学杂志,2017,32(04):60-63.[doi:10.3969/j.issn.1671-7414.2017.04.017]
 LI Hong,TANG Liu,ZHENG Lin,et al.Expression of microRNA-126 in Colon Cancer and Its Clinical Significance[J].Journal of Modern Laboratory Medicine,2017,32(04):60-63.[doi:10.3969/j.issn.1671-7414.2017.04.017]
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结肠癌组织中microRNA-126表达及临床意义()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年04期
页码:
60-63
栏目:
论著
出版日期:
2017-07-25

文章信息/Info

Title:
Expression of microRNA-126 in Colon Cancer and Its Clinical Significance
文章编号:
1671-7414(2017)04-060-04
作者:
李 洪唐 浏郑 淋刘 娴
琼海市人民医院检验科,海南琼海 571400
Author(s):
LI HongTANG LiuZHENG LinLIU Xian
Department of Clinical Laboratory,Qionghai People's Hospital,Hainan Qionghai 571400,China
关键词:
结肠癌 microRNA-126 生物学功能
分类号:
R735.35; R730.43
DOI:
10.3969/j.issn.1671-7414.2017.04.017
文献标志码:
A
摘要:
目的 探讨microRNA-126在结肠癌组织中的表达及生物学意义。方法 收集104例结肠癌手术患者,实时定量聚合酶链反应(RT-PCR)检测结肠癌患者的结肠癌组织以及癌旁组织中microRNA-126的表达(共104对)。应用慢病毒表达载体构建microRNA-126细胞系,并进一步通过体外实验研究microRNA-126对结肠癌细胞增殖、迁移和侵袭能力的影响。结果 结肠癌组织中microRNA-126相对表达水平(0.63±0.11)明显低于癌旁组织(1.08±0.15),差异有统计学意义(t=14.561,P<0.01)。结肠癌组织中microRNA-126表达阳性率(61.5%)显著低于癌旁组织(86.5%),差异有统计学意义(χ2=16.908,P<0.05)。microRNA-126表达与结肠癌Dukes分期、浸润深度、有无淋巴结转移及远处转移明显相关(P<0.05)。在有基质胶的Transwell实验中,转染组细胞迁移数(11.26±4.85个)明显低于空白对照组(264.37±32.15个),差异有统计学意义(t=23.418,P<0.01)。无基质胶的Transwell实验中,转染组细胞迁移数(83.75±13.74个)也明显低于空白对照组(339.64±26.38个),差异有统计学意义(t=12.682,P<0.01)。microRNA-126可以抑制SW480细胞的增殖及其侵袭转移。结论 microRNA-126可明显抑制结肠癌细胞的发生发展,影响结肠癌细胞的生物学行为。
Abstract:
Objective To investigate the expression and biological significance of microRNA-126 in colon cancer tissue.MethodsThe expression of microRNA-126 in colon cancer tissues and adjacent tissues of colon cancer patients was detected by real-time quantitative polymerase chain reaction(RT-PCR)in 104 patients with colon cancer surgery(totalof 104 pairs).The lentiviral vector was used to construct microRNA-126 cell line,and the effects of microRNA-126 on proliferation,migration and invasion ofcolon cancer cells were further studied in vitro.Results The relative expression of microRNA-126 in colon cancer tissues(0.63±0.11)was significantly lower than that in adjacent tissues(1.08±0.15),the difference was statistically significant(t=14.561,P<0.01).The positive rateof microRNA-126 expression in colon cancer tissues(61.5%)was significantly lower than that in adjacent cancer tissues(86.5%)the difference was statistically significant(χ2=16.908,P<0.05).The expression of microRNA-126 wassignificantly correlated with Dukes stage,depth of invasion,lymph node metastasis and distant metastasis(P<0.05).In the Transwell experiment with matrix glue,the number of cell migration in transfection group(11.26±4.85)wassignificantly lower than that in blank control group(264.37±32.15),the difference was statistically significant(t=23.418,P<0.01).In the Transwellexperiment without matrix glue,the number of cell migration in transfection group(83.75±13.74)was significantly lower than that in blank control group(339.64±26.38),the difference was statistically significant(t=12.682,P<0.01).MicroRNA-126 could inhibit the proliferation and invasion of SW480 cells.Conclusion MicroRNA-126 can significantly inhibit thedevelopment of colon cancer cells and affect the biological behavior of colon cancer cells.

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备注/Memo

备注/Memo:
作者简介:李 洪(1979-),男,本科,主管检验师,从事临床医学检验研究,E-mail:lihong668@126.com。
更新日期/Last Update: 1900-01-01