[1]刘丽莎,李喆倩,谈 诚,等.川崎病并发冠脉损伤患儿血清活化HUVECs NF-κB促使MMP-9 mRNA水平上调的实验研究[J].现代检验医学杂志,2018,33(01):131-132,136.[doi:10.3969/j.issn.1671-7414.2018.01.001]
　LIU Li-sha,LI Zhe-qian,TAN Cheng,et al.Experimental Study on the Up-Regulation of NF- kappa B and MMP-9 mRNA Level in HUVECs Stimulated by Serum from Children with Coronary Artery Lesions of Kawasaki Disease[J].Journal of Modern Laboratory Medicine,2018,33(01):131-132,136.[doi:10.3969/j.issn.1671-7414.2018.01.001]
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川崎病并发冠脉损伤患儿血清活化HUVECs NF-κB促使MMP-9 mRNA水平上调的实验研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第33卷
期数:
2018年01期
页码:
131-132,136
栏目:
研究简报·实验技术
出版日期:
2018-02-05

文章信息/Info

Title:
Experimental Study on the Up-Regulation of NF- kappa B and MMP-9 mRNA Level in HUVECs Stimulated by Serum from Children with Coronary Artery Lesions of Kawasaki Disease
文章编号:
1671-7414(2018)01-131-03
作者:
刘丽莎; 李喆倩; 谈 诚; 于汉卿
南京医科大学附属儿童医院检验科,南京 210008
Author(s):
LIU Li-sha; LI Zhe-qian; TAN Cheng; YU Han-qing
Department of Clinical Laboratory, Children's Hospital of Nanjing Medical University,Nanjing 210008,China
关键词:
川崎病;  冠状动脉损伤;  核因子-κB;  基质金属蛋白酶9
分类号:
R725.5; R392.11
DOI:
10.3969/j.issn.1671-7414.2018.01.001
文献标志码:
A
摘要:
目的 探讨脐静脉内皮细胞(human umbilical vein endothelial cells,HUVECs)在川崎病(kawasaki disease,KD)并发冠状动脉损伤(coronary artery lesions,CALs)患儿血清作用下,核因子-κB(nuclear factor-kappa B,NF-κB)对基质金属蛋白酶9(matrix metalloproteases 9,MMP9)mRNA转录的影响。方法将HUVECs分为4组,分别为正常血清组(control组)、一般发热血清组(F组)、无冠脉损伤组(non-CALs组)和冠脉损伤组(CALs组)。免疫共沉淀(co-immunoprecipitation,ChIP)检测HUVECs中NF-κB p65与MMP-9启动子结合情况,RT-PCR检测HUVECs MMP-9mRNA水平。结果 与对照组相比,经KD患儿,特别是并发CALs患儿血清作用后,HUVECs NF-κB p65可直接结合在MMP-9启动子上,同时MMP-9 mRNA呈高表达。结论 HUVECs在KD并发CALs患儿血清作用下,NF-κB p65可直接结合在MMP-9启动子上促进其转录。
Abstract:
Abstract:Objective To investigate the relationship between nuclear factor-kappa B(NF-κB)and matrix metalloproteinase-9(MMP-9)mRNA in human umbilical vein endothelial cells(HUVECs)stimulated by the serum from children with coronary artery lesions of Kawasaki disease(KD). Methods HUVECs were cultured and were divided into 4 groups:normalserum group,general fever group,Non-CALs group and CALs group.Co-Immunoprecipitation(ChIP)was used to detect the relationship between NF-κB and MMP-9,and RT-PCR was used to detect the mRNA level of MMP-9.ResultsCompared with control groups,NF-κB p65 could bind the promoterof MMP-9 in HUVECs cultured with 10% serum from KD patients with coronary artery lesions.The mRNA level of MMP-9 was also up-regulated.Conclusion NF-κB p65 can promote the transcription of MMP-9 in HUVECsinduced by the serum from KD patients with coronary artery lesions.

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备注/Memo

备注/Memo:
基金项目:南京市医学科技发展基金(YKK13131)。 作者简介:刘丽莎(1984-),女,硕士,主管技师,研究方向:川崎病病理损伤与信号转导,E-mail:58065421@qq.com。
更新日期/Last Update: 2018-02-07