[1]韩慕天,程洪波,王家雄,等.不孕女性生殖道UU,CT,NG和MG感染状况分析及不同检测方法结果比较[J].现代检验医学杂志,2018,33(01):137-140.[doi:10.3969/j.issn.1671-7414.2018.01.001]
　HAN Mu-tian,CHENG Hong-bo,WANG Jia-xiong,et al.Analysis of UU,CT,NG and MG in Infertile Women and Comparison of Different Detection Methods[J].Journal of Modern Laboratory Medicine,2018,33(01):137-140.[doi:10.3969/j.issn.1671-7414.2018.01.001]
点击复制

不孕女性生殖道UU,CT,NG和MG感染状况分析及不同检测方法结果比较()
分享到：

《现代检验医学杂志》[ISSN:/CN:]

卷:
第33卷
期数:
2018年01期
页码:
137-140
栏目:
研究简报·实验技术
出版日期:
2018-02-05

文章信息/Info

Title:
Analysis of UU,CT,NG and MG in Infertile Women and Comparison of Different Detection Methods
文章编号:
1671-7414(2018)01-137-04
作者:
韩慕天; 程洪波; 王家雄; 沈丽燕; 王改改; 宋 丹; 杨慎敏; 王馥新; 许咏乐; 王 玮; 李 红; 史轶超
南京医科大学附属苏州医院生殖遗传中心,江苏苏州 215000
Author(s):
HAN Mu-tian; CHENG Hong-bo; WANG Jia-xiong; SHEN Li-yan; WANG Gai-gai;  SONG Dan; YANG Shen-min; WANG Fu-xin; XU Yong-le; WANG Wei; LI Hong; SHI Yi-chao
Reproductive Heredity Center,the Suzhou Hospital Affiliated to Nanjing Medical University,Jiangsu Suzhou 215000,China
关键词:
支原体;  衣原体;  非淋菌性尿道炎;  核酸恒温扩增检测法
分类号:
R691.3
DOI:
10.3969/j.issn.1671-7414.2018.01.001
文献标志码:
A
摘要:
目的 分析不孕症女性生殖道常见微生物的分布状况,比较核酸恒温扩增检测技术(SAT)与培养法或乳胶法解脲支原体(UU)、沙眼衣原体(CT)、淋病奈瑟菌(NG)和生殖支原体(MG)检出的差异。方法 2016年6月～9月于南京医科大学附属苏州医院生殖中心就诊的467例女性不孕患者,年龄20～48(31.52±6.83)岁,分析UU,CT,NG和MG的感染分布状况。选择其中352例行辅助生殖技术的女性患者,年龄21～46(30.67±6.67)岁,取生殖道拭子标本,一份以培养法或乳胶法检测UU或CT。另一份用SAT法进行检测,根据实验结果评估两种方法检测相应微生物的敏感度和特异度差异。结果467例不孕女性生殖道拭子检测结果中UU阳性率最高62.53%(292/467),CT阳性率1.93%(9/467),NG阳性率0.21%(1/467),MG阳性率1.71%(8/467)。与正常对照组[23.81%(25/105)]相比不孕女性组UU感染率更高(χ2=52.01,P<0.01)。对352例行辅助生殖技术的不孕女性患者用不同检测方法检测UU,CT,UU拭子培养的阳性率为48.9%,UU-SAT检测阳性率为63.9%,SAT法阳性率高于培养法。拭子培养和SAT检测结果经配对四格表χ2检验,差异具有统计学意义(χ2=41.93,P<0.01),显示SAT法检测UU具有更高的敏感度。CT-SAT检测阳性率为1.71%,CT乳胶法检测阳性率为0.28%。乳胶法检测结果与SAT结果相比差异具有统计学意义(Fisher确切概率法统计分析,P<0.05),SAT阳性率和敏感度更高。结论 不孕女性生殖道常见病原体以UU最为多见,其次为CT和MG。SAT法与培养法或乳胶法检测UU和CT相比较,前者具有更高的敏感度。
Abstract:
Abstract:Objective To analyze the distribution of pathogens in the genital tract of infertile female,and comparing traditional methodswith simultaneous amplification and testing(SAT)in the detection of UU,CT,NGand MG.Methods 467 female infertility patients were selected from the reproductive center of Suzhou Hospital Affiliated to Nanjing Medical University between June and September 2016 to analyze the distribution of UU,CT,MG and NG.The age was between 20 to 48 years old(mean 31.52±6.83 years old).352 cases of female patients with assisted reproductive technology wereselected,aged from 21 to 46 years old(mean 30.67±6.67 years old).The swabswere tested by traditional methods or SAT.The sensitivity and specificity of the methods in detecting the pathogens were evaluated according to the experimental results.Results Among the 467 infertile women, the number of UU positive cases was the highest,the positive rate was 62.53%(292/467),the positive rate of CT was 1.93%(9/467)and the positive rate of NG was0.21%(1/467),and the positive rate of MG was 1.71%(8/467).UU infection rate was higher in infertile women than normal control group 23.81%(25/105)(χ2=52.01,P<0.01).352 cases of female patients with assisted reproductive technology were selected for further analysis.For UU detection,the positiverate of swab samples detected by liquid culture was 48.9%,while the positive rate detected by SAT was 63.9%.Obviously the positive rate of SAT was higher than that of liquid culture.Swab culture and SAT results were analyzed by pairedχ2 test(χ2=41.93,P<0.01).The positive rate of CT-SAT was 1.71%,and the positive rate of CT-latex method was 0.28%.There was significant difference between CT latex method and SAT(Fisher exact probabilistic methodstatistical analysis,P<0.005),which indicated that SAT method had a higher sensitivity.The positive rate(1.7%)and sensitivity(100%)of SAT were also higher than that of traditional method.Conclusion UUwas the most common pathogen in female reproductive tract pathogens,followed by CT andMG.The SAT method has higher sensitivity than the conventional method in detecting of UU and CT.

参考文献/References:

[1] Babu G,Singaravelu BG,Srikumar R,et al.Comparative study onthe vaginal flora and incidence of asymptomatic vaginosis among healthy women and in women with infertility problems of reproductive age[J].J Clin Diagn Res,2017,11(8):DC18-DC22.
[2] 《非淋菌性尿道炎病原学诊断专家共识》编写组,中华医学会男科学分会.非淋菌性尿道炎病原学诊断专家共识[J].中华男科学杂志,2016,22(11):1038-1043. 《Expert Consensus on Pathogen Diagnosis of Non-Gonococcal Urethritis》Write Group,Chinese Medical Association male science branch.Expert consensus on pathogen diagnosis of non-gonococcal urethritis[J].NationalJournal of Andrology,2016,22(11):1038-1043.
[3] Taylor-Robinson D.Mollicutes in vaginal microbiology:Mycoplasma hominis,Ureaplasma urealyticum,Ureaplasma parvum and Mycoplasma genitalium[J].Res Microbiol,2017,168(9/10):875-881.
[4] Xie QZ,Xu WM,Qi QR,et al.Patients with cervical Ureaplasma Urealyticum and Chlamydia Trachomatis infection undergoing IVF/ICSI-ET:The needfor new paradigm[J].J Huazhong Univ Sci Technolog(Med Sci),2016,36(5):716-722.
[5] Horner PJ,Blee K,Falk L,et al.2016 European gui-deline on the management of non-gonococcal urethritis[J].Int J STD AIDS,2016,27(11):928-937.
[6] Takanashi M,Ito S,Kaneto H,et al.Development and clinical applicationof an InvaderPlus○R assay for the detection of genital mycoplasmas[J].J Infect Chemother,2015,21(7):516-519.
[7] Gerovassili A, Marcandona O, Asimakopoulos B,et al.Relationship betweenChlamydia-Ureaplasma-Mycoplasma Genital detection with semen concentration and motility among greek men[J].Int J Fertil Steril,2017,11(2):130-133.
[8] Rudkjobing VB,Thomsen TR,Xu Y,et al.Comparing culture and molecular methods for the identification of microorganisms involved in necrotizing soft tissue infections[J].BMC Infect Dis,2016,16(1):652.
[9] Schwebke JR,Rompalo A,Taylor S,et al.Re-evaluating the treatment ofnongonococcal urethritis:emphasizing emerging pathogens-a randomized clinicaltrial[J].Clin Infect Dis,2011,52(2):163-170.
[10] Nakashima K,Shigehara K,Kawaguchi S,et al.Pre-valence of human papillomavirus infection in the oropharynx and urine among sexually active men:a comparative study of infection by papillomavirus and other organisms,including Neisseria gonorrhoeae,Chlamydia trachomatis,Mycoplasma spp,and Ureaplasma spp[J].BMC Infect Dis,2014,14(2):43-48.
[11] Salmeri M,Valenti D,La Vignera S,et al.Prevalence of Ureaplasma urealyticum and Mycoplasma hominis infection in unselected infertile men[J].J Chemother,2012,24(2):81-86.
[12] Seifoleslami M,Safari A,Khayyat KM.Prevalence of Ureaplasma urealyticum and Mycoplasma hominis in high vaginal swab samples of infertile females[J].Iran Red Crescent Med J,2015,17(12):e16823.
[13] Phillips-Howard PA, Nyothach E, Ter Kuile FO,et al.Menstrual cups andsanitary pads to reduce school attrition,and sexually transmitted and reproductive tract infections:a cluster randomised controlled feasibility study in rural Western Kenya[J].BMJ Open,2016,6(11):e013229.
[14] Menon S, Timms P, Allan JA,et al.Human and pathogen factors associatedwith chlamydia trachomatis-related infertility in women[J].Clin Microbiol Rev,2015,28(4):969-985.
[15] Foschi C,Salvo M,Cevenini R,et al.Chlamydia trachomatis antimicrobial susceptibility in colorectal and endocervical cells[J].J Antimicrob Chemother,2017,34(6):322-330.
[16] Salfa MC,Suligoi B,Italian STI Laboratory-based surveillance WorkingGroup.Prevalence of Chlamydia trachomatis,Trichomonas vaginalis and Neisseria gonorrhoeae Based on Data Collected by a Network of Clinical Microbiology Laboratories,in Italy[J].Adv Exp Med Biol,2016,901:47-57.
[17] Taylor-Robinson D,Jensen JS,Svenstrup H,et al.Difficulties experienced in defining the microbial cause of pelvic inflammatory disease[J].Int J STD AIDS,2012,23(1):18-24.
[18] Cicinelli E,Matteo M,Tinelli R,et al.Chronic endometritis due to common bacteria is prevalent in women with recurrent miscarriage as confirmed by improved pregnancy outcome after antibiotic treatment[J].Reprod Sci,2014,21(5):640-647.
[19] Gaydos CA.Mycoplasma genitalium:accurate diagnosis is necessary for adequate treatment[J].J Infect Dis,2017,216(suppl 2):S406-S411.
[20] Levina GA,Barkhatova OI,Gorina LG,et al.Unusual forms of persistenceof Mycoplasma hominis in organism of infected humans[J].Zh Mikrobiol Epidemiol Immunobiol,2012(4):104-109.
[21] Centers for Disease Control and Prevention.Recommendations for the laboratory-based detection of Chlamydia trachomatis and Neisseria gonorrhoeae-2014[J].MMWR Recomm Rep,2014,63(RR-02):1-19.
[22] Martens MG,Fine P,Fuller D,et al.Clinical evaluation of a new Pap test-based method for screening of Chlamydia trachomatis and Neisseria gonorrhoeae using liquid-based cytology media[J].South Med J,2013,106(9):506-512.
[23] 郑 渠,刘 玮,张国巍,等.RNA恒温扩增法与培养法检测解脲脲原体结果比较分析[J].中华男科学杂志,2017,23(8):717-721. Zheng Q,Liu W,Zhang GW,et al.RNA simultaneous and amplificationtesting versus the culture method for detecting ureaplasma urealyticum infection[J].National Journal of Andrology,2017,23(8):717-721.
[24] Ling Q, Song QX, Feng JL,et al.Prevalence of Chlamydia trachomatis,Neisseria gonorrhoeae,Mycoplasma genitalium and Ureaplasma urealyticum infections using a novel isothermal simultaneous RNA amplification testing methodin infertile males[J].Ann Clin Microbiol Antimicrob,2017,16(1):45-54. 收稿日期:2017-09-19 修回日期:2017-11-27

相似文献/References:

[1]梁有才a,张苑珊b,范菲楠,等.泌尿生殖道支原体采用固体联合液体培养新方案的实验研究[J].现代检验医学杂志,2017,32(03):126.[doi:10.3969/j.issn.1671-7414.2017.03.034]
　LIANG You-caia,ZHANG Yuan-shanb,FAN F ei-nan,et al.Research on New Experimental Scheme of Mycoplasma Solid Culture Combined with Liquid Culture[J].Journal of Modern Laboratory Medicine,2017,32(01):126.[doi:10.3969/j.issn.1671-7414.2017.03.034]

备注/Memo

备注/Memo:
基金项目:江苏省遗传医学重点学科基金(FXK201305); 苏州市临床重点病种诊疗技术专项(lczx201411); 苏州市临床医学中心(szzx201505)。 作者简介:韩慕天(1989-),男,免疫学硕士,初级检验技师,研究方向:脂肪酸的免疫调控以及多囊卵巢综合征发病机制研究,E-mail:793563556@qq.com。 通讯作者:史轶超,E-mail:syc@androl.cn。
更新日期/Last Update: 2018-02-07