[1]鲁 军,程歆琦,禹松林,等.异嗜性抗体干扰化学发光方法检测cTnI的处理和分析[J].现代检验医学杂志,2018,33(02):101-104.[doi:10.3969/j.issn.1671-7414.2018.02.001]
　LU Jun,CHENG Xin-qi,YU Song-lin,et al.Processing and Analysis of cTnI Detected by Heterophilic Antibodies Interference with Chemiluminescence[J].Journal of Modern Laboratory Medicine,2018,33(02):101-104.[doi:10.3969/j.issn.1671-7414.2018.02.001]
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异嗜性抗体干扰化学发光方法检测cTnI的处理和分析()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第33卷
期数:
2018年02期
页码:
101-104
栏目:
研究简报·实验技术
出版日期:
2018-04-05

文章信息/Info

Title:
Processing and Analysis of cTnI Detected by Heterophilic Antibodies Interference with Chemiluminescence
文章编号:
1671-7414(2018)02-101-04
作者:
鲁 军; 程歆琦; 禹松林; 侯立安; 金 成; 由婷婷
中国医学科学院 北京协和医学院 北京协和医院检验科,北京 100730
Author(s):
LU Jun; CHENG Xin-qi; YU Song-lin; HOU Li-an; JIN Cheng; YOU Ting-ting
Department of Clinical Laboratory,Peking Union Medical CollegeHospital, Chinese Academic Medical Science and Peking Union Medical College,Beijing100730,China
关键词:
心肌肌钙蛋白I;  干扰;  异嗜性抗体
分类号:
R392.3
DOI:
10.3969/j.issn.1671-7414.2018.02.001
文献标志码:
A
摘要:
目的 分析和处理异嗜性抗体干扰心肌肌钙蛋白I(cTnI)免疫测定所导致的假阳性结果。方法 分别采用稀释法、更换检测系统(Beckman,Siemens和Abbott)及异嗜性抗体阻断剂对1例65岁男性患者血浆样本进行处理及检测,比较分析稀释一定倍数、更换检测系统和异嗜性抗体阻断剂处理前后的cTnI结果。结果 使用Beckman系统检测该患者血浆稀释前以及稀释2倍、4倍、8倍的cTnI结果分别为4.60,4.03,3.45和2.62 ng/ml; 使用Beckman,Siemens和Abbott系统检测该患者血浆cTnI的结果分别为4.60,0.023和0.022 ng/ml; 使用Beckman系统检测异嗜性抗体阻断剂处理前后的血浆cTnI结果分别为4.60和0.106 ng/ml。结论该患者血浆cTnI检测受到了异嗜性抗体的干扰,更换检测系统或使用异嗜性抗体阻断剂可以有效解决此类干扰作用。
Abstract:
Abstract:Objective To analyze and deal with false positive results caused by heterophile antibody interference immunoassay.Methods A 65-year-old male patient's plasma samples were processedby dilution method,heterophile antibody blockers and tested using different detection systems(Beckman,Siemens and Abbott),respectively.The results obtained by multiplied dilution and different detection systems as well as pre-and post-processed by heterophilic antibody blockers were compared and analyzed.Results The Beckman system was used to detect plasma cTnI in patients before dilution and multiplied diluted at 2,4 and 8 times,with the results of 4.60,4.03,3.45 and 2.62 ng/ml,respectively.The cTnI results of the patient's plasma determined by Beckman,Siemens and Abbott system were 4.60,0.023 and 0.022 ng/ml,respectively.The plasma cTnI results of before and after heterophilic antibody blockers processing determined by Beckman system were4.60 and 0.106 ng/ml respectively.Conclusion This cTnI assay of the patient's plasma was interfered by heterophile antibodies,andaltering the detection system or utilizing heterophile antibody blockers can solve such interference effectively.

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备注/Memo

备注/Memo:
作者简介:鲁 军(1988-),男,大学本科,检验技师,现主要从事临床生化及免疫工作。 通讯作者:程歆琦,研究生,副主任医师,E-mail: 18610207700@qq.com。
更新日期/Last Update: 2018-04-16