[1]程永红,肖小平.中重度颅脑损伤并发脑梗死患者血清miRNA-124与miRNA-210表达的临床意义[J].现代检验医学杂志,2018,33(03):83-87.[doi:10.3969/j.issn.1671-7414.2018.03.021]
　CHENG Yong-hong,XIAO Xiao-ping.Clinical Application in Detecting Serum microRNA-124 and microRNA-210 in the Moderately Severe Craniocerebral Injury Complicated with the Traumatic Cerebral Infarction[J].Journal of Modern Laboratory Medicine,2018,33(03):83-87.[doi:10.3969/j.issn.1671-7414.2018.03.021]
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中重度颅脑损伤并发脑梗死患者血清miRNA-124与miRNA-210表达的临床意义()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第33卷
期数:
2018年03期
页码:
83-87
栏目:
论著
出版日期:
2018-07-17

文章信息/Info

Title:
Clinical Application in Detecting Serum microRNA-124 and microRNA-210 in the Moderately Severe Craniocerebral Injury Complicated with the Traumatic Cerebral Infarction
文章编号:
1671-7414(2018)03-083-05
作者:
程永红1; 肖小平2
1.商洛市中心医院检验科, 陕西商洛 726000,2.西安市第八医院检验科,西安 710061
Author(s):
CHENG Yong-hong1; XIAO Xiao-ping2
1.Department of Clinical Laboratory, the Central Hospital of Shangluo City,Shaanxi Shangluo 726000,China; 2.Department of Clinical Laboratory,the Eighth Hospital of Xi'an City,Xi'an 710061,China
关键词:
颅脑损伤;  外伤性脑梗死;  miRNA-124;  miRNA-210
分类号:
R651.15; R392.11
DOI:
10.3969/j.issn.1671-7414.2018.03.021
文献标志码:
A
摘要:
目的 探讨血清微小RNA(microRNA,miRNA)-124和miRNA-210表达在中重度颅脑损伤并发外伤性脑梗死中的临床应用价值。方法 收集商洛市中心医院和西安市第八医院住院治疗的57例中重度颅脑损伤患者的血清及临床资料,依据是否发生外伤性脑梗死分为梗死组22例,未梗死组35例,并设立对照组(24例),采用实时荧光定量聚合酶链反应(Real-time-PCR,RT-PCR)技术检测血清miRNA-124和miRNA -210的表达。比较分析血清miRNA-124和miRNA-210的变化与外伤性脑梗死的关系。结果 血清miRNA-124和miRNA-210在梗死组、未梗死组和对照组的表达分别为(0.90±0.18,0.39±0.07,0.18±0.03)和(0.95±0.20,0.42±0.10,0.21±0.04)。与对照组比较,血清miRNA-124和miRNA-210在未梗死组和梗死组的表达显著上调,差异均有统计学意义(t=22.99,23.67,均P<0.01; t=35.37,36.93,均P<0.01)。两标志物在梗死组的表达显著高于未梗死组,差异均有统计学意义(t=23.27,25.12,均P<0.01)。大脑前动脉(ACA)、大脑中动脉(MCA)及大脑后动脉(PCA)在梗死组、未梗死组和对照组的平均流速分别为(36.9±7.6,45.3±8.6,33.9±5.5)cm/s,(60.3±7.9,79.2±12.7,49.6±7.2)cm/s,(51.3±7.7,70.5±11.9,43.1±6.9)cm/s.ACA,MCA及PCA在未梗死组的平均流速与对照组比较明显增快,差异有统计学意义(t=12.23,13.10,13.16,均P<0.01)。ACA,MCA和PCA在梗死组的平均流速分别与未梗死组及对照组比较明显减慢,差异有统计学意义[(t=11.30,12.67,12.09,均P<0.01),(t=11.37,11.52,11.09,均P<0.01)]。miRNA-124和miRNA-210在梗死组的表达具有正相关性(r=0.629,均P<0.01),它们分别与梗死组ACA,MCA和PCA的平均流速具有负相关性[(r=-0.653,-0.620,-0.613,均P<0.01),(r=-0.679,-0.656,-0.637,均P<0.01)],而与未梗死组ACA,MCA和PCA的平均流速则具有正相关性[(r=0.640,0.596,0.607,均P<0.01),(r=0.676,0.625,0.638,均P<0.01)]。结论 检测血清miRNA-124和miRNA-210的表达可预测外伤性脑梗死的发生及判断其危险程度,估计病情的发展状况。
Abstract:
Abstract:Objective To investigate the clinical application value of the levels of serum miRNA-124 and miRNA-210 in the moderately severe craniocerebral injury complicated with the traumatic cerebral infarction.Methods The serum and clinical data in 57 cases of patientswith the moderately severe craniocerebral injury from the Central Hospital ofShangluo City and the Eighth Hospital of Xi'an City were collected.Patients were divided into the infarction group(n=22)and non infarction group(n=35)in accordance with whether there happaned the traumatic cerebral infarction,and set up the control group(24 cases in control group),using the real-timefluorescent quantitative PCR(Real-time-PCR,RT-PCR)technology to detect the expression of serum miRNA-124 and miRNA-210 in comparative analysing the relationship between the changes of serum miRNA-124,-210 and the traumatic cerebral infarction.Results In the infarcted group,non-infarcted group and control group,the expression of miRNA-124 were 0.90±0.18,0.39±0.07 and 0.18±0.03,which of miRNA-210 were 0.95±0.20,0.42±0.10 and 0.21±0.04.The expression of serum miRNA-124 and miRNA -210 in the the infarcted group and non-infarcted group significantly increased compared withthose in the control group,the differences had statistical significances(t=22.99,23.67,all P<0.01 and t=35.37,36.93,all P<0.01).The expression of the two markers were higher in the infarction group than thosein the non-infarction group(t=23.27,25.12,all P<0.01).In the infarction group,non infarction group and the control group,the average velocityof the anterior cerebral artery(ACA),middle cerebral artery(MCA)and posterior cerebral artery(PCA),respectively,were(36.9±7.6,60.3±7.9,51.3±7.7)cm/s,(45.3±8.6,79.2±12.7,70.5±11.9)cm/s.(33.9±5.5,49.6±7.2,43.1±6.9)cm/s.The average velocity of MCA,PCA and ACA was obviously increased in non infarction group compared with those in the control group,and the differences were statistically significant(t=12.23,13.10,13.16,all P<0.01).However,the significantly decreased presences were also shown in the infarction group compared with those in the non-infarction group andthe control group(t=11.30,12.67,12.09,all P<0.01 and t=11.37,11.52,11.09,all P<0.01).In the infarction group,the expression ofthe miRNA-124 and miRNA-210 had a positive correlation(r=0.629,all P<0.01).In the infarction group,the negative relation had been found betweenthe expression of two markers and the average flow velocity of ACA,MCA and PCA(r=-0.653,-0.620,-0.613,all P<0.01 and r=-0.679,-0.656,-0.637,all P<0.01),while in the non-infarction group,the positverelation had been presented between them(r=0.640,0.596,0.607,all P<0.01; r=0.676,0.625,0.638,all P<0.01).ConclusionDetecting the expression of serum miRNA-124,miRNA-210 can predict the occurrence of traumatic cerebral infarction,and judge the dangerous degree in estimating the development of the disease.

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备注/Memo

备注/Memo:
作者简介:程永红(1968-),女,大专学历,主管检验师,研究方向:脑卒中的基因诊断,E-mail:554283592@qq.com。 通讯作者:肖小平(1968-),男,本科学历,主管检验师,研究方向:脑卒中的基因诊断,E-mail:3248089878@qq.com。
更新日期/Last Update: 2018-04-16