[1]卢美红,鞠少卿,丛 辉,等.南通地区健康人群血清NSE电化学发光法检测参考区间的调查[J].现代检验医学杂志,2018,33(04):59-62.[doi:10.3969/j.issn.1671-7414.2018.04.015]
　LU Mei-hong,JU Shao-qing,CONG Hui,et al.Investigate of Serum NSE Reference Range of Healthy People in Nantong with Electrochemiluminescence[J].Journal of Modern Laboratory Medicine,2018,33(04):59-62.[doi:10.3969/j.issn.1671-7414.2018.04.015]
点击复制

南通地区健康人群血清NSE电化学发光法检测参考区间的调查()
分享到：

《现代检验医学杂志》[ISSN:/CN:]

卷:
第33卷
期数:
2018年04期
页码:
59-62
栏目:
论著
出版日期:
2018-08-30

文章信息/Info

Title:
Investigate of Serum NSE Reference Range of Healthy People in Nantong with Electrochemiluminescence
文章编号:
1671-7414(2018)04-059-04
作者:
卢美红; 鞠少卿; 丛 辉; 杨曙梅; 祝文彩
南通大学附属医院医学检验科,江苏南通 226001
Author(s):
LU Mei-hong; JU Shao-qing; CONG Hui; YANG Shu-mei; ZHU Wen-cai
Department of Laboratory Medicine,the Affiliated Hospital of Nantong University,Jiangsu Nantong 226001,China
关键词:
神经元特异性烯醇化酶;  年龄;  性别;  参考区间
分类号:
R446.112
DOI:
10.3969/j.issn.1671-7414.2018.04.015
文献标志码:
A
摘要:
目的 调查和建立南通地区表观健康人群神经元特异性烯醇化酶(neuron-specific enolase,NSE)的参考值区间。方法 选取2017年1月～2018年1月南通大学附属医院体检中心3 874例健康体检者作为研究对象,其中男性2 767例,女性1 107例,通过问卷调查和体格检查排除神经系统、血液病、炎症、肿瘤等疾病,采用罗氏cobase 411全自动电化学发光仪检测NSE水平。根据性别、年龄将受检者分组(男女各7组:<30岁,30～39岁,40～49岁,50～59岁,60～69岁,70～79岁,>80岁),对结果进行统计学分析,建立相应的参考区间。分析性别、年龄分组后的组间差异是否有统计学意义; 将获得的参考区间与试剂盒提供的生物参考区间进行比较,并进行参考区间的验证。结果 NSE水平呈正态分布,男性血清NSE水平(15.79±3.52ng/ml)明显高于女性(13.79±3.04 ng/ml),差异有统计学意义(t=3.102,P=0.037)。但独立样本t检验进行各年龄组间两两比较差异无统计学意义(P>0.05)。采用(-overx)&#177; 1.96s确定参考区间,NSE参考区间为8.47～21.07 ng/ml,男性为8.72～21.36 ng/ml,女性为8.06～20.10 ng/ml,对该研究建立的NSE参考区间进行验证符合标准。结论 初步建立了适合该地区表观健康人群的血清NSE参考区间。NSE的参考区间有性别差异,根据不同性别建立的参考区间,可为疾病的诊断和治疗提供准确的依据。
Abstract:
Abstract:Objective To investigate and establish a reference range of neuron-specific enolase(NSE)in apparently healthy population inNantong region.Methods A total of 3 874 healthy examinees from the medical examination center of the Affiliated Hospital of Nantong University were included in the study of which 2 767 were males and 1 107 were females.NSE was detected using a Roche Cobase 411 automatic electrochemiluminometer.There were classified according to gender and age(divided into 7 age groupsfor men and women:<30 years,30～39 years,40～49 years,50～59 years,60～69 years,70～79 years,>80 years).The results were statistically analyzed and the corresponding reference intervals was established.Statistically significant differences between groups after gender and age were analyzed.Compared the reference interval obtained with the instrument manual biological reference interval. Results NSE levels were normally distributed.Here was a statistically significant difference in NSE levels between males and females(t=3.102,P=0.037).There was no significant difference between the two age groups in the independent sample t test(P>0.05).Determine the referenceinterval using (-overx)&#177; 1.96s,NSE reference interval 8.47～21.07 ng/ml,male NSE reference interval 8.72～21.36 ng/ml.Female NSE reference interval(8.06～20.10 ng/ml),and there was a difference with the reference interval provided by the manufacturer.Validation of reference intervals meets standards. Conclusion A preliminary set of serum NSE reference intervals suitable for apparently healthy people in the region was established.The reference range of NSE in healthy people in this region was related to gender,soit is possible to establish their own reference ranges according to different genders to provide an accurate basis for the diagnosis and treatment of diseases.

参考文献/References:

[1] Bharosay A,Bharosay VV,Saxena K,et al.Role of brain biomarkerin predicting clinical outcome in hypertensive cerebrovascular ischemic stroke[J].Indian J Clin Biochem,2018,33(2):178-183.
[2] Haque A,Polcyn R,Matzelle D,et al.New insights into the role of neuron-specific enolase in neuro-inflammation,neurodegeneration,and neuroprotection[J].Brain Sci,2018,8(2):33.
[3] 李岸凤,赵咏梅,刘运秋.NSE和ProGRP表达水平与小细胞肺癌病理分期的相关性研究[J].实用癌症杂志,2018,33(2):177-180. Li AF,Zhao YM,Liu YQ.Correlation research of NSE and ProGRP levelwith different pathological stages of small cell lung cancer[J].The Practical Journal of Cancer,2018,33(2):177-180.
[4] 乔潜林,白新学,刘海玉,等.脑脊液和血清中PCT与NSE及CRP检测在颅内感染诊断中的价值分析[J].中华医院感染学杂志,2017,27(11):2469-2472. Qiao QL,Bai XX,Liu HY,et al.Analysis of the value of cerebrospinal fluid and serum PCT,NSE and CRP detection in the diagnosis of intracranial infection[J].Chinese Journal of Nosocomiol,2017,27(11):2469-2472.
[5] 赵车冬,闫玉珠,吴文婧,等.血清CEA,CYFRA21-1,NSE水平在肺癌诊断与分型中的应用价值[J].现代检验医学杂志,2016,31(6):59-61,65. Zhao CD,Yan YZ,Wu WJ,et al.Application value of serum CEA,CYFRA21-1 and NSE levels in the diagnosis and typing of lung cancer[J].Journal ofModern Laboratory Medicine,2016,31(6):59-61,65.
[6] 杨银忠,张建英,程文霞,等.血液HSP90a,CEA,NSE,CYFRA21-1及SCCA联合检测对肺癌的诊断价值研究[J].中国现代医学杂志,2017,27(12):60-64. Yang YZ,Zhang JY,Cheng WX,et al.Value of blood HSP90a,CEA,NSE,CYFRA21-1 and SCCA in diagnosis lung cancer[J].Chinese Journal of Modern Medicine,2017,27(12):60-64.
[7] 何 正,罗世翊,魏 玲,等.CEA,NSE,CA125,CYFRA21-1在肺癌中的检测及意义[J].系统医学,2017,2(7):7-9. He Z,Luo SY,Wei L,et al.Test and significance of CEA,NSE,CA125,CYFRA21-1 in lung cancer[J].System Medicine,2017,2(7):7-9.
[8] Clinical and Laboratory Standards Institute.CLSI C28-A3.Defining,establishing,and verifying reference intervals in the clinical laboratory[S].Wayne:PA,CLSI C28-A3,2008.
[9] 中华人民共和国国家卫生和计划生育委员会.WS/T402-2012临床实验室检验项目参考区间的制定[S].北京:中国标准出版社,2012. National Health and Family Planning Commission of the People's Republic of China.WS/T402-2012 Define and determine the reference intervals in clinical laboratory[S].Beijing:China Standard Press,2012.
[10] 何建平,王小艳,梁志乐.缺血性脑卒中血清神经元特异性烯醇化酶的变化及其意义[J].实用医学杂志,2017,33(4):612-615. He JP,Wang XY,Liang ZL.Changes of serum neuron-specific enolase in ischemic stroke and its significance[J].The Journal of Practical Medicine,2017,33(4):612-615.
[11] 李玉艳,梁琳琳,刘晓勇.颅脑损伤患者脑脊液和血清神经元特异性烯醇化酶及髓鞘碱性蛋白水平变化及临床意义[J].国际检验医学杂志,2017,38(10):1338-1340. Li YY,Liang LL,Liu XY.Changes and clinical significance of serum and cerebrospinal fluid NSE and MBP in patients with craniocerebral injury[J].International Journal of Laboratory Medicine,2017,38(10):1338-1340.
[12] Liu L,Teng J,Zhang L,et al.The combination of the tumor markers suggests the histological diagnosis of lung cancer[J].Biomed Res Int,2017,2017:2013989.
[13] Huang L,Zhou JG,Yao WX,et al.Systematic review and meta-analysis ofthe efficacy of serum neuron-specific enolase for early small cell lung cancerscreening[J].Oncotarget,2017,8(38):64358-64372.
[14] Volaric D,Flego V,Bauhar G,et al.Diagnostic value of tumour markersin pleural effusions[J].Biochem Med(Zagreb),2018,28(1):010706.
[15] 丁志勇,谢奇朋,陈 翔,等.儿童和成人血清神经元特异性烯醇化酶正常值的比较研究[J].实用医学杂志,2010,26(24):4518-4520. Ding ZY,Xie QP,Chen X,et al.Comparison of the normal value of neuro-specific enolase in sera between children and adults[J].The Journal of Practical Medicine,2010,26(24):4518-4520.

相似文献/References:

[1]张 萍a,王红义b,杨迎桂a,等.血清CEA,ProGRP,NSE和CYFRA21-1联合检测在肺癌诊断中的应用研究[J].现代检验医学杂志,2016,31(02):56.[doi:10.3969/j.issn.1671-7414.2016.02.017]
　ZHANG Pinga,WANG Hong-yib,YANG Ying-guia,et al.Clinical Application of Combined Detection of CEA,ProGRP,NSE and CYFRA21-1 in Diagnosis of Lung Cancer[J].Journal of Modern Laboratory Medicine,2016,31(04):56.[doi:10.3969/j.issn.1671-7414.2016.02.017]
[2]李 霞,刘小红,杨 乐.不同年龄阶段癫痫患儿奥卡西平 活性代谢产物血药浓度的临床价值[J].现代检验医学杂志,2015,30(01):143.[doi:10.3969/j.issn.1671-7414.2015.01.043]
　LI Xia,LIU Xiao-hong,YANG Le.Clinical Values of Blood Serum Concentration of MHD in Children with Epilepsy of Different Ages[J].Journal of Modern Laboratory Medicine,2015,30(04):143.[doi:10.3969/j.issn.1671-7414.2015.01.043]
[3]王洋一,胡宏章.在不同性别及年龄间糖化血红蛋白水平差异分析[J].现代检验医学杂志,2017,32(02):123.[doi:10.3969/j.issn.1671-7414.2017.02.034]
　WANG Yang-yi,HU Hong-zhang.Differential Analysis of Glycosylated Hemoglobin in Different Sex and Age[J].Journal of Modern Laboratory Medicine,2017,32(04):123.[doi:10.3969/j.issn.1671-7414.2017.02.034]
[4]邢延芳,张 艳,艾江波,等.延安市宝塔区40岁以上常住人口随机抽样筛查脑卒中高危人群血清Hcy水平与性别及年龄差异[J].现代检验医学杂志,2018,33(05):111.[doi:10.3969/j.issn.1671-7414.2018.05.030]
　XING Yan-fang,ZHANG Yan,AI Jiang-bo,et al.Homocysteine Comparison in Different Gender and Age Groups with High Risk of Stroke of Permanent Residents over Forty Years Old in Baota District of Yan'an[J].Journal of Modern Laboratory Medicine,2018,33(04):111.[doi:10.3969/j.issn.1671-7414.2018.05.030]
[5]易晓榕,张明忠,田卫华,等.湖北省恩施土家族苗族自治州不孕妇女不同年龄阶段血清抗苗勒管激素水平研究[J].现代检验医学杂志,2019,34(04):96.[doi:10.3969/j.issn.1671-7414.2019.04.023]
　YI Xiao-rong,ZHANG Ming-zhong,TIAN Wei-hua,et al.Changes of Anti-Mullerian Hormone Levels in Infertile Women of Different Ages in Enshi Minority Areas[J].Journal of Modern Laboratory Medicine,2019,34(04):96.[doi:10.3969/j.issn.1671-7414.2019.04.023]
[6]梁培松,王结珍,陈 康,等.孕早期年龄,BMI 和PAPPA 水平综合评价对GDM 的预测价值[J].现代检验医学杂志,2020,35(01):133.[doi:10.3969/j.issn.1671-7414.2020.01.035]
　LIANG Pei-song,WANG Jie-zhen,CHEN Kang,et al.Predictive Value of Comprehensive Evaluation of Early Pregnant Age,BMI and PAPPA Levels to the GDM[J].Journal of Modern Laboratory Medicine,2020,35(04):133.[doi:10.3969/j.issn.1671-7414.2020.01.035]
[7]彭　琦a,程少鹏b,陈辅萍a.非小细胞肺癌组织中NSE 的基因表达及与患者临床特征的相关性研究[J].现代检验医学杂志,2020,35(03):36.[doi:10.3969/j.issn.1671-7414.2020.03.008]
　PENG Qia,CHENG Shao-pengb,CHEN Fu-pinga.Correlation between NSE Gene Expression and Serum NSE Level in Tumor Tissues and Clinical Characteristics of Patients with Non-Small Cell Lung Cancer[J].Journal of Modern Laboratory Medicine,2020,35(04):36.[doi:10.3969/j.issn.1671-7414.2020.03.008]
[8]徐翠香,王建华,武　敏,等.胃癌患者血清CA724，NSE和 PGR水平联合检测对肿瘤临床病理分期及淋巴结转移的诊断价值[J].现代检验医学杂志,2021,36(01):6.[doi:10.3969/j.issn.1671-7414.2021.01.002]
　XU Cui-xiang,WANG Jian-hua,WU Min,et al.Diagnostic Value of Combined Detection of Serum CA724, NSE and PGR Levels in Patients with Gastric Cancer for Tumor Clinical Pathological Stage and Lymph Node Metastasis[J].Journal of Modern Laboratory Medicine,2021,36(04):6.[doi:10.3969/j.issn.1671-7414.2021.01.002]
[9]王妮娜,李晓花,陈敏丽,等.肺癌患者血清NSE，SCC，CA125及 CYFRA21-1水平检测在不同病理类型早期诊断和化疗疗效评价中的应用价值[J].现代检验医学杂志,2021,36(01):33.[doi:10.3969/j.issn.1671-7414.2021.01.009]
　WANG Ni-na,LI Xiao-hua,CHEN Min-li,et al.Application Value of Serum NSE, SCC, CA125 and CYFRA21-1 in Patients of Lung Cancer for Early Diagnosis and Evaluation of Chemotherapy Efficacy in Patients with Different Pathological Types[J].Journal of Modern Laboratory Medicine,2021,36(04):33.[doi:10.3969/j.issn.1671-7414.2021.01.009]
[10]朱　娇a,孙国才b,刘素荣a,等.食管癌患者血清神经元特异性烯醇化酶与长链非编码RNA ITGA9-AS1水平联合检测的实验诊断价值研究[J].现代检验医学杂志,2021,36(05):62.[doi:10.3969/j.issn.1671-7414.2021.05.014]
　ZHU Jiaoa,SUN Guo-caib,LIU Su-ronga,et al.Experimental Diagnostic Value of Combined Detection of SerumNeuron-Specific Enolase and Long Non-Coding RNA ITGA9-AS1 inPatients with Esophageal Cancer[J].Journal of Modern Laboratory Medicine,2021,36(04):62.[doi:10.3969/j.issn.1671-7414.2021.05.014]

备注/Memo

备注/Memo:
基金项目:江苏省“六大人才高峰”项目(WS-066); 南通市科技项目(MS12017008-1)。 作者简介:卢美红(1975-),女,硕士,副主任技师,主要研究方向:临床免疫学检验,E-mail:alu522@126.com。 通信作者:祝文彩:男,主任技师,E-mail:5840712620@qq.com。
更新日期/Last Update: 2018-08-16