[1]朱　娇a,孙国才b,刘素荣a,等.食管癌患者血清神经元特异性烯醇化酶与长链非编码RNA ITGA9-AS1水平联合检测的实验诊断价值研究[J].现代检验医学杂志,2021,36(05):62-64.[doi:10.3969/j.issn.1671-7414.2021.05.014]
　ZHU Jiaoa,SUN Guo-caib,LIU Su-ronga,et al.Experimental Diagnostic Value of Combined Detection of SerumNeuron-Specific Enolase and Long Non-Coding RNA ITGA9-AS1 inPatients with Esophageal Cancer[J].Journal of Modern Laboratory Medicine,2021,36(05):62-64.[doi:10.3969/j.issn.1671-7414.2021.05.014]
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食管癌患者血清神经元特异性烯醇化酶与长链非编码RNA ITGA9-AS1水平联合检测的实验诊断价值研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第36卷
期数:
2021年05期
页码:
62-64
栏目:
论　著
出版日期:
2021-10-14

文章信息/Info

Title:
Experimental Diagnostic Value of Combined Detection of SerumNeuron-Specific Enolase and Long Non-Coding RNA ITGA9-AS1 inPatients with Esophageal Cancer
文章编号:
1671-7414（2021）05-062-04
作者:
朱　娇1a; 孙国才1b; 刘素荣1a; 王英英2
(1. 兵器工业五二一医院a. 肿瘤血液病科;b. 老年病科，西安 710065;2. 陕西中医药大学附属医院肿瘤二科，陕西咸阳 712000)
Author(s):
ZHU Jiao1a;  SUN Guo-cai1b;  LIU Su-rong1a;  WANG Ying-ying2
（1a.Department of Oncology Hematology;1b. Department of Geriatric, 521 Hospital of Norinco Group, Xi’an 710065, China;2.the Second Oncology Department, Affiliated Hospital of Shaanxi University of Chinese Medicine, Xi’an 712000, China）
关键词:
神经元特异性烯醇化酶; 长链非编码RNA; 食管癌
分类号:
R735.1；R730.43
DOI:
10.3969/j.issn.1671-7414.2021.05.014
文献标志码:
A
摘要:
目的　探究食管癌(esophagus cancer, EC)患者血清神经元特异性烯醇化酶(neuronspecific enolase, NSE)与长链非编码RNA(long non-coding RNA, lncRNA) ITGA9-AS1水平联合检测的实验诊断价值。方法　选择2018年5月~2019年7月兵器工业五二一医院收治的112例EC可疑病变患者为研究对象，经病理学检查确诊67例EC患者为EC组，45例食管良性病变为食管良性病变组。采用电化学发光免疫分析仪检测NSE水平，采用实时荧光定量PCR(quantitative Real-time PCR, qRT-PCR)检测血清lncRNA ITGA9-AS1的水平。Pearson相关分析分析血清NSE，lncRNA ITGA9-AS1水平与EC患者病理分期、肿瘤浸润深度的相关性。结果　EC组患者血清NSE（22.71±3.03μg/L vs 2.98±0.22μg/L）和lncRNA ITGA9-AS1（0.88±0.21 vs 0.64±0.14）的表达水平高于食管良性病变组，差异具有统计学意义 (t=13.33，6.41，均P=0.00)。III期EC患者血清NSE和lncRNA ITGA9-AS1水平显著高于I期和II期，差异具有统计学意义(P＜0.01)，另外肿瘤浸润深度T4血清NSE和lncRNA ITGA9-AS1水平显著高于T1，差异具有统计学意义(P＜0.01)。Pearson相关性分析结果显示，血清NSE，lncRNA ITGA9-AS1水平与EC患者病理分期、肿瘤浸润深度呈显著正相关(r=0.142，P=0.005和r=0.171，P=0.013)。血清NSE，lncRNA ITGA9-AS1诊断敏感度均较低，但均具有较高的特异度。联合检测在EC中有较高的敏感度和特异度，分别为82.1%和83.2%。结论　血清NSE和lncRNA ITGA9-AS1定量检测可用于EC的诊断，联合检测可提高诊断的敏感度。
Abstract:
Objective　To explore the diagnostic value of combined detection of serum neuronspecific enolase (NSE) and longnon-coding RNA(lncRNA) ITGA9-AS1 in patients with esophagus cancer (EC). Methods　A total of 112 patients withsuspected EC lesions admitted to 521 Hospital of Norinco Group from May 2018 to July 2019 were selected as the study subjects.67 patients with EC confirmed by pathological examination were classified as EC group, and 45 patients with benign esophageallesions were classified as esophageal benign lesions group. The level of NSE was detected by electrochemiluminescenceimmunoanalyzer, and the level of serum lncRNA ITGA9-AS1 was detected by quantitative real-time PCR (qRT-PCR). Results　Theexpression levels of serum NSE(22.71±3.03μg/L vs 2.98±0.22μg/L) and lncRNA ITGA9-AS1 (0.88±0.21 vs 0.64±0.14) inEC group were higher than those in esophageal benign lesions group, and the difference was statistically significant (t=13.33,6.41, all P=0.00). The serum levels of NSE and lncRNA ITGA9-AS1 in stage III EC patients were significantly higher than thosein stage I and II, with statistical significance (P<0.01). In addition, the serum levels of NSE and lncRNA ITGA9-AS1 in T4patients with tumor invasion depth were significantly higher than those in T1, with statistical significance (P <0.01). Pearsoncorrelation analysis showed that serum NSE, lncRNA ITGA9-AS1 levels were significantly positively correlated withpathological stage and tumor invasion depth in EC patients (r=0.142, P=0.005 and r=0.171, P=0.013). Serum NSE and lncRNA ITGA9-AS1 had low diagnostic sensitivity, but both had high specificity and sensitivity. The sensitivity and specificity of thecombined test in EC were 82.1% and 83.2%, respectively. Conclusion　Quantitative detection of serum NSE and lncRNAITGA9-AS1 could be used in the diagnosis of EC, and the combined detection can improve the sensitivity of the diagnosis.

参考文献/References:

[1] 孟茜茜，张子凡，程志远，等．食管癌肿瘤标志物研究及临床应用进展[J]. 中国实用内科杂志, 2019,39(7):634-639.MENG Qianqian, ZHANG Zifan, CHENG Zhiyuan,et al. Advances in research and clinical applicationof tumor markers in esophageal cancer [J]. ChineseJournal of Practical Internal Medicine, 2019, 39(7):634-639.
[2] 马箐, 马山蕊, 王孟, 等．MicroRNAs 与食管癌关系的研究进展[J]. 中国肿瘤, 2017, 26(5):371-377.MA Qing, MA Shanrui, WANG Meng, et al. Researchprogress on relationship between microRNAs andesophageal cancer [J]. China Cancer, 2017, 26(5):371-377.
[3] 吴丹, 贺远龙. 早期食管癌内镜下切除术后食管狭窄的防治进展[J]. 实用医学杂志, 2016, 32(11):1882-1884.WU Dan, HE Yuanlong. Progress in the preventionand treatment of esophageal stenosis after endoscopicresection of early esophageal cancer [J]. The Journal ofPractical Medicine, 2016, 32(11): 1882-1884.
[4] 熊利芬，李蜀豫．食管黏膜脱落细胞DNA 倍体联合血清IL-12,IL-16,IL-17 检测在食管癌诊断中的应用[J]. 解放军医药杂志, 2019, 31(11):24-27.XIONG Lifen, LI Shuyu. DNA ploidy in esophagealmucosa exfoliated cells combined with serum IL-12, IL-16 and IL-17 detection in diagnosis of patientswith esophageal cancer [J]. Medical & PharmaceuticalJournal of Chinese People’s Liberation Army, 2019,31(11):24-27.
[5] 李国良, 朱明闯, 熊鹏, 等. 长链非编码RNA 在食管癌中的研究进展[J]. 实用医学杂志,2018, 34 (24):4015-4018.LI Guoliang, ZHU Mingchuang, XIONG Peng,et al. Research progress of long non-coding RNAin esophageal cancer [J]. The Journal of PracticalMedicine, 2018, 34(24):4015-4018.
[6] 贾小婷, 罗利云, 郑国沛, 等.LncRNA ITGA9-AS1抑制乳腺癌细胞增殖并增强其对顺铂的敏感性[J].中国生物化学与分子生物学报, 2017,33(7):706-711.JIA Xiaoting, LUO Liyun, ZHENG Guopei, et al.LncRNA ITGA9-AS1 inhibits proliferation andenhances sensitivity of breast cancer cells to cDDP[J]. Chinese Journal of Biochemistry and MolecularBiology, 2017,33(7):706-711.
[7] 杜园园，李鹏，郭旭霞．血清C- 反应蛋白联合细胞角蛋白19 片段抗原、鳞状上皮细胞癌相关抗原及神经元特异性烯醇化酶检测在食管癌患者中的临床应用[J]. 中国卫生检验杂志, 2020, 30(9):1033-1035,1042.DU Yuanyuan, LI Peng, GUO Xuxia. Clinicalapplication of combined detection of serumCRP,CYFRA21-1, SCC and NSE in esophageal cancer[J]. Chinese Journal of Health Laboratory Technology,2020, 30(09):1033-1035,1042.
[8] 彭彦, 王燕, 李峻岭, 等. 血清NSE，ProGRP 和LDH 在小细胞肺癌诊断治疗中的作用[J]. 中国肺癌杂志, 2016, 19(9):590-594.PENG Yan, WANG Yan, LI Junling, et al. Utility ofNSE, ProGRP and LDH in diagnosis and treatment inpatients with small cell lung cancer [J]. Chinese Journalof Lung Cancer , 2016, 19(9):590-594.
[9] 王宝平，张泓，余绍兵，等．血清CA125，NSE 及24h 尿VMA 检测在小儿神经母细胞瘤诊治中的应用价值[J]. 癌症进展, 2019, 17(7):837-839,868.WANG Baoping, ZHANG Hong, YU Shaobing, et al.The application value of detecting serum CA125, NSEand 24-hour urine VMA in diagnosis and treatment ofpediatric neuroblastoma [J]. Oncology Progress, 2019,17(7):837-839,868.
[10] 屈雪, 秦晓松. 胃泌素释放肽前体、癌胚抗原和神经元特异性烯醇化酶在食管癌诊断中的价值研究[J]. 中国肿瘤外科杂志, 2020, 12(2):144-147.QU Xue, QIN Xiaosong. Diagnostic value ofprogastrin-releasing peptide, carcinoembryonic antigenand neuron specific enolase in esophageal cancer [J].Chinese Journal of Surgical Oncology, 2020, 12(2):144-147.
[11] 陈津，彭伟，崔兆磊，等．食管癌放疗患者血清涎液化糖链抗原水平检测在预测放射性肺炎的风险评估[J]. 现代检验医学杂志, 2020, 35(3):126-128,156.CHEN Jin, PENG Wei, CUI Zhaolei, et al. SerumKL-6 predicts the risk of pneumonitis inductionafter radiotherapy of esophageal cancer [J]. Journalof Modern Laboratory Medicine, 2020, 35(3):126-128,156.
[12] 肖小平，张熊，秦光明．食管癌和良性食管疾病患者血浆miRNA-21 和miRNA-143 检测的临床应用研究[J]. 现代检验医学杂志, 2017, 32(4):72-75,139.XIAO Xiaoping, ZHANG Xiong, QIN Guangming.Clinical research of detecting plasma miRNA-21 andmiRNA-143 for identifying early esophageal cancerand benign esophageal diseases [J]. Journal of ModernLaboratory Medicine, 2017, 32(4):72-75,139.

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备注/Memo

备注/Memo:
作者简介：朱娇（1987-），女，硕士，主治医师，研究方向：肿瘤血液病的临床诊疗，E-mail:aa362484318@126.com。
通讯作者：王英英（1986-），女，硕士，主治医师，研究方向：常见肿瘤的治疗，E-mail:listars555@163.com。
更新日期/Last Update: 1900-01-01