[1]李瑜珍a,郑 琳b,张惠媚a,等.不同浓度葡萄糖、氯化钠及氯化铁对铜绿假单胞菌生物膜形成的影响研究[J].现代检验医学杂志,2018,33(04):131-133.[doi:10.3969/j.issn.1671-7414.2018.04.036]
　LI Yu-zhena,ZHENG Linb,ZHANG Hui-meia,et al.Study on the Effects of Different Concentration Glucose,Sodium Chloride,Ferric Chloride on the Biofilm of Pseudomonas Aeruginosa[J].Journal of Modern Laboratory Medicine,2018,33(04):131-133.[doi:10.3969/j.issn.1671-7414.2018.04.036]
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不同浓度葡萄糖、氯化钠及氯化铁对铜绿假单胞菌生物膜形成的影响研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第33卷
期数:
2018年04期
页码:
131-133
栏目:
研究简报·实验技术
出版日期:
2018-08-30

文章信息/Info

Title:
Study on the Effects of Different Concentration Glucose,Sodium Chloride,Ferric Chloride on the Biofilm of Pseudomonas Aeruginosa
文章编号:
1671-7414(2018)04-131-03
作者:
李瑜珍a; 郑 琳b; 张惠媚a; 张春雷a
深圳市中医院a.检验科; b.中药制剂研究重点实验室,广东深圳 518034
Author(s):
LI Yu-zhena; ZHENG Linb; ZHANG Hui-meia; ZHANGChun-leia
a.Department of Clinical Laboratory; b.Key Laboratoryof Traditional Chinese Medicine Research,Shenzhen TCM Hospital,Guangdong Shenzhen 518034,China
关键词:
葡萄糖;  氯化钠;  氯化铁;  铜绿假单胞菌生物膜
分类号:
R378.991; R446.5
DOI:
10.3969/j.issn.1671-7414.2018.04.036
文献标志码:
A
摘要:
深圳市中医院a.检验科; b.中药制剂研究重点实验室,广东深圳 518034
Abstract:
Abstract:Objective To study the effect of different concentrations glucose,sodium chloride,ferric chloride on the biofilm producingcapacity of Pseudomonas aeruginosa,and provide new strategy for the P.aeruginosa biofilm prevention/treatment and optimization of the condition of P.aeruginosa biofilm experiment model in vitro.Methods P.aeruginosa were co-cultured with glucose at the concentration of 0,27.75,55.51,83.26 and 111.01 mmol/L,with NaCl at the concentration of 0,85.56,171.12,256.67 and 342.23 mmol/L,and with FeCl3 at the concentrationof 0,0.77,1.54,3.09 and 6.17 mmol/L respectively.Compared with the control groups with no glucose,NaCl or FeCl3,the biofilm producing capacity ofP.aeruginosa were tested by semi-quantitative crystal violet method in vitro.Results The groups of 55.51 mmol/L glucose,85.56 mmol/L,171.12 mmol/L sodim chloride and 3.09 mmol/L FeCl3 was respectivelycompared with the control group the differences were statistically significant(P=0.020,0.036,0.017,0.041).Conclusion 55.51 mmol/L glucose,85.56 mmol/L NaCl,171.12 mmol/L NaCl,3.09 mmol/L FeCl3treatment could significantly induce the biofilm producing of P.aeruginosa.To prevent the P.aeruginosa biofilm producing,patient infected with P.aeruginosa should control blood glucose and reduce sodium intake.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:深圳市科技计划项目(JCYJ20150401163247217),广东省医学科学基金项目(A2017402)。 作者简介:李瑜珍(1972-),女,大学本科,学士学位,副主任技师,主要从事临床微生物学研究,E-mail.liyuzhen0803@163.com。
更新日期/Last Update: 2018-08-16