[1]李 君,曹立瀛,侯金友,等.河北省唐山地区人群红细胞A2B亚型分布情况及其分子机制的研究[J].现代检验医学杂志,2019,34(02):20-22.[doi:10.3969/j.issn.1671-7414.2019.02.006]
　LI Jun,CAO Li-ying,HOU Jin-you,et al.Study on Distribution and Molecular Mechanism of A2B SubtypeIndividuals in Tangshan Region of Hebei Province[J].Journal of Modern Laboratory Medicine,2019,34(02):20-22.[doi:10.3969/j.issn.1671-7414.2019.02.006]
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河北省唐山地区人群红细胞A2B亚型分布情况及其分子机制的研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第34卷
期数:
2019年02期
页码:
20-22
栏目:
论著
出版日期:
2019-05-11

文章信息/Info

Title:
Study on Distribution and Molecular Mechanism of A2B SubtypeIndividuals in Tangshan Region of Hebei Province
文章编号:
1671-7414(2019)02-020-04
作者:
李 君1; 曹立瀛1; 侯金友1; 张 慧1; 邹红蕊1; 孙 超2; 崔振超3
(1.开滦总医院输血科,河北唐山 063000; 2.唐山市中心血站,河北唐山 063000; 3.秦皇岛市中心血站,河北秦皇岛 066000)
Author(s):
LI Jun1; CAO Li-ying1;  HOU Jin-you1; ZHANG Hui1; ZOU Hong-rui1; SUN Chao2; CUI Zhen-chao3
(1.Blood Transfusion Branch,Kailuan General Hospital,Hebei Tangshan 063000,China; 2.Tangshan Central Blood Station,Hebei Tangshan 063000,China; 3.Qinhuangdao Central Blood Station,Hebei Qinhuangdao 066000,China)
关键词:
A2B亚型;  基因测序;  分子机制;  输血安全性
分类号:
R457.11
DOI:
10.3969/j.issn.1671-7414.2019.02.006
文献标志码:
A
摘要:
目的 研究河北省唐山地区人群A2B亚型分布情况及分子机制,探讨输血前开展A2B亚型检测的临床意义。方法 收集2017年11月～2018年3月住院、门诊、急诊及体检的AB型标本,采用抗-A1试剂筛选A2B亚型。采用PCR技术扩增A2B标本ABO基因第6～7外显子并进行Sanger法双向测序分析。结果 535例AB型样本检出12例A2B表型标本(2.24%)。基因测序显示2例标本基因型为ABO*A2.01/ABO*B.01,6例为ABO*A2.05/ABO*B.01,1例为ABO*BA.02/ABO*O.01.02,3例为ABO*A1.02/ABO*B.01。人群ABO*A2.05等位基因频率为0.56%,ABO*A2.01为0.19%。结论 河北省唐山地区人群存在一定比例A2B亚型,其分子机制存在异质性; 输血前开展A2B亚型检测能提升输血安全性。
Abstract:
Objective To investigate the distribution of A2B subtype individuals and their molecular characteristic in Tangshan Region of Hebei Province.Methods The individuals with AB phenotypewere collected in outpatients,in-patients,emergency department and physicalexamination department from November 2017 to March 2018.A2B phenotype was screened with Anti-A1.Then the exon 6 to 7 of ABO in the A2B phenotype individuals was amplified by polymerase chain reaction and sequenced bidirectionally with Sanger method.Results A2B phenotypic specimens werefound in 12 out of 535 AB phenotype individuals(2.24%).The sequence results were showed that genotypes of 2 individuals were ABO*A2.01/ABO*B.01,6 were ABO*A2.05/ABO*B.01, 1 was ABO*BA.02/ABO*O.01.02, and 3 were ABO*A1.02/ABO*B.01.The frequency of ABO*A2.05 allele was 0.56% and ABO*A2.01 was 0.19% in the Tangshan population.Conclusion There was a certain proportion of A2B subtypes in Tangshan Region of Hebei Province,and its molecular mechanism was heterogeneous.Testing A2B subtype before transfusion can improve the safety of blood transfusion.

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备注/Memo

备注/Memo:
作者简介:李 君(1985-),女,硕士,从事临床输血工作,E-mail:278800026@qq.com。 收稿日期:2019-02-10 修回日期:2019-02-23 页码：22+26
更新日期/Last Update: 2019-04-30