[1]王 磊,孙文利,刘 瑞,等.HPLC-MS/MS法检测骨髓移植患者全血环孢素A(CsA)浓度及与AM1和AM4N代谢产物的相关性研究[J].现代检验医学杂志,2019,34(02):52-55.[doi:10.3969/j.issn.1671-7414.2019.02.014]
　WANG Lei,SUN Wen-li,LIU Rui,et al.Correlation of the Concentration of Whole Blood Cyclosporine A(CsA)Detected by HPLC-MS/MS Method and Metabolites of AM1and AM4N in Bone Marrow Transplant Patients[J].Journal of Modern Laboratory Medicine,2019,34(02):52-55.[doi:10.3969/j.issn.1671-7414.2019.02.014]
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HPLC-MS/MS法检测骨髓移植患者全血环孢素A(CsA)浓度及与AM1和AM4N代谢产物的相关性研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第34卷
期数:
2019年02期
页码:
52-55
栏目:
论著
出版日期:
2019-05-11

文章信息/Info

Title:
Correlation of the Concentration of Whole Blood Cyclosporine A(CsA)Detected by HPLC-MS/MS Method and Metabolites of AM1and AM4N in Bone Marrow Transplant Patients
文章编号:
1671-7414(2019)02-052-05
作者:
王 磊1; 孙文利1; 刘 瑞1; 王红春1; 刘红星1; 2
(1.河北燕达陆道培医院,河北三河 065201; 2.北京陆道培血液病研究院,北京 100176)
Author(s):
WANG Lei1; SUN Wen-li1; LIU Rui1; WANG Hong-chun1; LIU Hong-xing1; 2
(1.Hebei Yanda Ludaopei Hospital,Hebei Sanhe 065201,China; 2.Beijing Ludaopei Institute of Hematology,Beijing 100176,China)
关键词:
环孢素;  高效液相色谱-串联质谱法;  血药浓度;  代谢产物
分类号:
R617; Q503
DOI:
10.3969/j.issn.1671-7414.2019.02.014
文献标志码:
A
摘要:
目的 探索环孢素A(CsA)在骨髓移植患者代谢产物AM1和AM4N与母药CsA的关系,为CsA在临床合理使用提供支持。方法 用高效液相色谱-串联质谱法(HPLC-MS/MS)对骨髓移植患者个体内和个体间的全血样本进行CsA,AM1和AM4N血药浓度检测。结果 CsA个体内代谢:AM1与CsA相关性不好(r=0.79),AM4N与CsA和AM1相关性均较好(r>0.9),代谢产物AM1和AM4N含量及比率各异; CsA个体间代谢:个体间CsA和AM1的相关性较差(r=0.59),AM4N和CsA及AM1在个体间不存在相关性(r<0.5),代谢产物AM1和AM4N含量及比率各异。结论 CsA代谢存在个体差异; HPLC-MS/MS法可用于骨髓移植患者CsA血药浓度检测。
Abstract:
Objective To explore the relationship among AM1,AM4N and CsA in bone marrow transplant patients,and provide support for therational use of CsA in clinical practice.Methods HPLC-MS/MS method was used to detect the concentrations of CsA,AM1 and AM4N in whole blood samples(both within and between individuals)of bone marrow transplant patients.Results Internal metabolism of CsA:the correlation between AM1 and CsA was not good(r=0.79),the correlation between AM4Nand CsA and AM1 was good(r>0.9),and the content and ratio of metabolitesAM1 and AM4N were different.Interindividual metabolism of CsA:there was poorcorrelation between CsA and AM1 among individuals(r=0.59),and there was nocorrelation between AM4N,CsA and AM1 among individuals(r<0.5).The content and ratio of metabolites AM1 and AM4N were different.Conclusion There were individual differences in CsA metabolism.HPLC-MS/MS method can be used to detect the blood concentration of CsA in patients with bone marrow transplantation.

参考文献/References:

[1] FURUKAWA T, KURASAKI-IDA T, MASUKO M,et al.Pharmacokinetic and pharmacodynamic analysis of cyclosporine A(CsA)to find the best single time point for the monitoring and adjusting of CsA dose using twice-daily 3-h intravenous infusions in allogeneic hematopoietic stem cell transplantation[J].International Journal of Hematology,2010,92(1):144-151.
[2] 朱愿超,梁良,赵明,等.再生障碍性贫血患者环孢素A血药浓度监测的回顾性分析[J].临床药物治疗杂志,2015,13(5):20-24. ZHU Yuanchao,LIANG Liang,ZHAO Ming,et al.Retrospective analysis of blood concentration monitoring of cyclosporine A in patients with aplastic anemia[J].Journal of Clinical Pharmacotherapy,2015,13(5):20-24.
[3] 王翔岩,钟旭丽,宋泽亮,等.儿童造血干细胞移植后环孢素A血药浓度监测[J].临床药物治疗杂志,2015,13(6):21-24. WANG Xiangyan,ZHONG Xuli,SONG Zeliang,et al.Therapeutic drug monitoring of cyclosporine A in children hematopoietic stem cell transplantation[J].Medication Journal Clinical,2015,13(6):21-24.
[4] 陈英,叶冬梅,蓝梅,等.急性抗宿主病细胞因子基因多态性与环孢素A的关联性研究[Z].广西壮族自治区人民医院,2017. CHEN Ying,YE Dongmei,LAN Mei,et al.Association between cytokine gene polymorphism and cyclosporine A in acute versus host disease[Z].Guangxi Zhuang Autonomous Region People's Hospital,2017.
[5] 王磊,孙文利,李培余,等.环孢素A血药浓度检测方法比较分析[J/CD].中华临床医师杂志(电子版),2017,11(3):417-421. WANG Lei,SUN Wenli,LI Peiyu,et al.Comparative analysis of cyclosporine blood concentration detection methods[J/CD].Chinese Journal of Clinicians(Electronic Edition),2017,11(3):417-421.
[6] 沈陈军,陈新贵,王勇.荧光偏振免疫分析法与高效液相色谱法检测环孢素A血药浓度的倒方差法Meta分析[J].安徽医药,2018,22(7):1256-1262. SHEN Chenjun,CHEN Xingui,WANG Yong.Comparison between FPIA and HPLC method in monitoring cyclosporin A blood concentration based on pearson correlation coefficient:a meta analysis[J].Anhui Medical and Pharmaceutical Journal,2008,22(7):1256-1262.
[7] 孙搏,郭彦琨,高君伟,等.CYP3A基因多态性与肾移植术后不同时期环孢素浓度剂量个体差异的相关性研究[J].中国药师,2017,20(1):91-93. SUN Bo,GUO Yankun,GAO Junwei,et al.Effect CYP3A polymorphism oncycosporine individual dosage and blood concentration in renal transplant recipients[J].China Pharmacist,2017,20(1):91-93.
[8] 王磊,刘红星,孙文利.HPLC-MS/MS法在骨髓移植患者全血CSA及AM1浓度测定中的应用[J].重庆医学,2017,46(23):3234-3237. WANG Lei,LIU Hongxing,SUN Wenli.The application of HPLC-MS/MS method in the determination of whole blood concentration of concentration of scyclosporine A and AM1 in bone marrow transplant patient[J].Chongqing Medical Journal,2017,46(23):3234-3237.
[9] 赵驻军,李亚,张翠欣.TDx检测环孢素A血药浓度时探针冲洗步骤的简化[J].现代检验医学杂志,2005,20(4):39. ZHAO Zhujun,LI Ya,ZHANG Cuixin.Probe flushing procedure for TDx detection of cyclosporine A blood concentration[J].Journal of Modern Laboratory Medicine,2005,20(4):39.
[10] 王磊,孙文利,李培余,等.Elecsys与HPLC-MS/MS检测骨髓移植患者环孢素A血药浓度的相关性及其应用意义[J].中华临床实验室管理电子杂志,2018,6(2):99-103.WANG Lei,SUN Wenli,LI Peiyu,et al.Correlation of Elecsys and HPLC-MS/MS method in the determination of blood cyclosporine A concentration in bone marrow transplant patients and its application[J].Chinese Journal of Clinical Laboratory Management,2018,6(2):99-103.

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备注/Memo

备注/Memo:
作者简介:王 磊(1984-),男,硕士,助理研究员,主要从事医院药学研究工作,E-mail:rosie1982@163.com。 通讯作者:刘红星,男,医学硕士,副研究员,主要从事检验医学研究工作。 收稿日期:2019-02-01 修回日期:2019-02-19 页码：55+129
更新日期/Last Update: 2019-04-30