[1]屈柯暄,周丽华,王淑霞,等.新生儿ABO溶血病微柱凝集法放散试验中放散液剂量优化研究[J].现代检验医学杂志,2019,34(03):121-123,127.[doi:10.3969/j.issn.1671-7414.2019.03.030]
　QU Ke-xuan,ZHOU Li-hua,WANG Shu-xia,et al.Study on Different Doses of Diffuser in Serological Testof ABO-Hemolytic Disease of Newborns[J].Journal of Modern Laboratory Medicine,2019,34(03):121-123,127.[doi:10.3969/j.issn.1671-7414.2019.03.030]
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新生儿ABO溶血病微柱凝集法放散试验中放散液剂量优化研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第34卷
期数:
2019年03期
页码:
121-123,127
栏目:
研究简报·实验技术
出版日期:
2019-06-20

文章信息/Info

Title:
Study on Different Doses of Diffuser in Serological Testof ABO-Hemolytic Disease of Newborns
文章编号:
1671-7414(2019)03-121-04
作者:
屈柯暄1; 周丽华2; 王淑霞1; 吕孟兴1
(1.昆明市儿童医院输血科,昆明 650228; 2.昆明市妇幼保健院检验科,昆明 650031)
Author(s):
QU Ke-xuan1; ZHOU Li-hua2; WANG Shu-xia1; Lv Meng-xing1
(1.Department of BloodTransfusion,Kunming Children's Hospital,Kunming 650228,China; 2.Department ofClinical Laboratory,Kunming Maternal and Child Health Care Hospital,Kunming 650031,China)
关键词:
新生儿ABO溶血病;  微柱凝集法;  放散试验;  放散液剂量;  阳性检出率
分类号:
R722.18; R457.1
DOI:
10.3969/j.issn.1671-7414.2019.03.030
文献标志码:
A
摘要:
目的 比较不同剂量放散液在ABO溶血病微柱凝集法放散试验中阳性检出率的差异,得到最佳放散液剂量。方法 收集2018年1～9月,临床诊断符合ABO溶血病的新生儿血液标本,采用微柱凝集法,根据微柱凝胶卡反应腔容量,选取低、中、高三种剂量的放散液进行平行试验。结果 微柱凝集法放散试验中不同剂量放散液(25 μl组,50 μl组,100 μl组)的阳性检出率分别为82.09%,82.77%和99.55%; 25 μl与50 μl两组间阳性检出率比较,χ2=0.0313,P>0.05,差异无统计学意义。100μl组与25μl和50μl两组间阳性检出率比较,差异均有统计学显著性意义(χ2=78.519 6,74.948 6,均P<0.001),故100 μl放散液组阳性检出率最佳。结论 在ABO溶血病微柱凝集法放散试验中,推荐使用100 μl剂量放散液,以提高放散试验阳性检出率。
Abstract:
Objective To compare the difference of positivedetection rate of different doses of radiation solution in neonatal hemolytic disease serological test,and obtain the best dosage of radiation solution inerythrocyte antibody dispersal test.Methods From January 2018 to September 2018,neonates with jaundice or hyperbilirubinemia,incompatibility of maternal and infant blood groups,and clinical symptoms conforming toABO-hemolysis were collected.Microcolumn agglutination method was used to test erythrocyte antibody diffusion in neonates with ABO-hemolytic disease.Threedoses of low,medium and highwere selected and administered respectively.Parallel experiments were conducted and the results were analyzed statistically.Results Among 441 cases clinically diagnosed as ABO-hemolyticdisease of newborn,the positive detection rates of different doses of radiation solution(25 μl,50μl and 100 μl groups)were 82.09%,82.77% and 99.55%.There was no significant difference between 25μl and 50 μl groups(χ2=0.031 3,P>0.05).The positive detection rates of 100 μl group and other dosage groups were significantly different(χ2=78.519 6,74.948 6,all P<0.001),and the positive detection rate of 100 μl radiation solution was the highest.Conclusion In the erythrocyte antibody diffusion test of ABO-hemolytic disease in neonates(microcolumn agglutination method),100 μl dose of radiation solution is recommended to improve the positive detection rate of hemolytic disease in neonates.

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备注/Memo

备注/Memo:
基金项目:昆明市科学技术局社会科技发展重点项目,项目编号2017-1-S-14363。 作者简介:屈柯暄(1983-),女,硕士,主管技师,主要从事输血免疫学研究,E-mail:qukexuan@etyy.cn。 通讯作者:吕孟兴(1977-),男,硕士,副主任医师,临床输血,E-mail:lvmengxing@etyy.cn。 收稿日期:2019-03-07 修回日期:2019-03-26
更新日期/Last Update: 2019-06-20