[1]梁晓东a,骆强翔a,张伟明b,等.精子在体外两种培养液获能的实验比较研究[J].现代检验医学杂志,2019,34(04):124-127.[doi:10.3969/j.issn.1671-7414.2019.04.030]
　LIANG Xiao-donga,LUO Qiang-xianga,ZHANG Wei-mingb,et al.Experimental Comparative Study on Capacitation of Sperm in Two Kinds of Culture Media in Vitro[J].Journal of Modern Laboratory Medicine,2019,34(04):124-127.[doi:10.3969/j.issn.1671-7414.2019.04.030]
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精子在体外两种培养液获能的实验比较研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第34卷
期数:
2019年04期
页码:
124-127
栏目:
研究简报·实验技术
出版日期:
2019-08-20

文章信息/Info

Title:
Experimental Comparative Study on Capacitation of Sperm in Two Kinds of Culture Media in Vitro
文章编号:
1671-7414(2019)04-124-04
作者:
梁晓东a; 骆强翔a; 张伟明b; 莫淦文a; 郭江华a; 廖勇彬a
(江门市中心医院a.生殖医学诊疗中心; b.病案科,广东江门 529000)
Author(s):
LIANG Xiao-donga; LUO Qiang-xianga; ZHANG Wei-mingb; MO Gan-wena;  GUO Jiang-huaa; LIAO Yong-bina
(a.Center of Reproductive Medicine Diagnosis and Treatment; b.Department of Medical Records,Jiangmen Central Hospital,GuangdongJiangmen 529000,China)
关键词:
精子获能;  培养液;  单唾液酸四己糖神经节苷脂
分类号:
R446.19
DOI:
10.3969/j.issn.1671-7414.2019.04.030
文献标志码:
A
摘要:
目的 比较研究精子在体外两种培养液获能的情况,对比两种精子获能培养液的质量。方法 按照精液分析检查要求和精液分析的结果收集该中心48份精液样本,采用密度梯度离心法分离精子,调整精子浓度后,把精子悬液分别置于Quinn's HTF 和Vitrolife IVF plus精子培养液中,分别在0,4,6,8和10h五个时间点取精子悬液进行单唾液酸四己糖神经节苷脂(GM1)的定位染色,并对发出荧光信号的精子进行分类计数,用混合效应模型比较两种培养液的荧光染色结果。结果 获能精子的顶体区发出绿色荧光,顶体区有荧光的精子百分率(AA%)随着时间延长而增加,混合效应模型对比分析两种培养液的AA%的结果显示两者差异无统计学意义。结论 荧光染色的结果显示获能精子的比例随时间变化而增加,两种培养液差别不大,提示都能提供有利于精子受精功能发挥的环境。
Abstract:
Objective To compare and analyze the quality oftwo kinds of sperm capacitation medium.Methods According to the results of semen analysis and semen analysis,48 semen samples were collected from the center,and the sperm were separated by density gradient centrifugation.After adjusting the sperm concentration,the sperm suspension was placed in Quinn's HTF and Vitrolife IVF plus sperm culture medium,respectively.The sperm suspension was taken at five time points of 0,4,6,8 and 10 h respectively for GM1 localization staining,and the sperm with fluorescent signal was classified and counted.The mixed effect model was used to compare the fluorescence staining results of the two culture medium.Results The acrosome area(AA)of enabled sperm emitted green fluorescence,and the percentage of fluorescent sperm in the acrosome area(AA%)increased with time.The results of comparative analysis of AA% of the two cultured nights by mixed effectmodel showed that there was no significant difference between the two cultured nights.Conclusion The results of fluorescence staining showed that the percentage of capacitated sperm increased with time,and both media could play the role of sperm capacitation.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:江门市医疗卫生领域科技计划项目(2015002)。 作者简介:梁晓东(1983-),男,硕士研究生,主管技师,研究方向:精子相关功能与生殖助孕技术,E-mail:229290147@qq.com。 通讯作者:廖勇彬,男,医学博士,主任医师,E-mail:836944528@qq.com。收稿日期:2019-04-25 修回日期:2019-05-05
更新日期/Last Update: 2019-07-30