[1]张 燕,王伟红,郝万鹏.乌鲁木齐汉族妇女亚甲基四氢叶酸还原酶基因变异与血清叶酸和同型半胱氨酸水平的相关性研究[J].现代检验医学杂志,2019,34(05):98-100.[doi:10.3969/j.issn.1671-7414.2019.05.024]
　ZHANG Yan,WANG Wei-hong,HAO Wan-peng.Study on the Correlation between the Variation of Methylenetetrahydrofolate Reductase Gene and Folate and Homocysteine in Urumqi Han Women[J].Journal of Modern Laboratory Medicine,2019,34(05):98-100.[doi:10.3969/j.issn.1671-7414.2019.05.024]
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乌鲁木齐汉族妇女亚甲基四氢叶酸还原酶基因变异与血清叶酸和同型半胱氨酸水平的相关性研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第34卷
期数:
2019年05期
页码:
98-100
栏目:
论著
出版日期:
2019-10-29

文章信息/Info

Title:
Study on the Correlation between the Variation of Methylenetetrahydrofolate Reductase Gene and Folate and Homocysteine in Urumqi Han Women
文章编号:
1671-7414(2019)05-098-04
作者:
张 燕1; 王伟红1; 郝万鹏2
(1.乌鲁木齐市第一人民医院检验科,乌鲁木齐 830011; 2.乌鲁木齐市温泉康复医院,乌鲁木齐 830017)
Author(s):
ZHANG Yan1; WANG Wei-hong1; HAO Wan-peng2
(1.Department of Clinical Laboratory,Urumqi First People's Hospital,Urumqi 830011,China; 2.Urumqi Hot Spring Rehabilitation Hospital,Urumqi 830017,China)
关键词:
亚甲基四氢叶酸还原酶;  基因;  叶酸;  同型半胱氢酸
分类号:
Q754
DOI:
10.3969/j.issn.1671-7414.2019.05.024
文献标志码:
A
摘要:
目的 探讨亚甲基四氢叶酸还原酶(MTHFR)基因C677T变异的特征及对叶酸和同型半胱氨酸(Hcy)浓度的影响,并分析叶酸与Hcy的相关性。方法 采取病例与对照研究的方法,选取110例MTHFR基因C677T位点TT基因型为观察组,同时根据纳入标准选对照组118例。通过PCR-金磁微粒层析法检测MTHFR基因多态性。采用电化学发光法测定血清中叶酸浓度,使用全自动生化分析,循环酸法检测血清中Hcy。结果 乌鲁木齐地区汉族女性MTHFR位点TT基因型的频率为24.5%。观察组叶酸均值为4.9±2.6 μg/L,Hcy均值为25.9±2.9 μmol/L,对照组叶酸与Hcy均值分别为15.5±1.9 μg/L和10.5±1.8 μmol/L,两组比较,差异均有统计学意义(t=8.356,9.835,均P<0.05)。观察组不良妊娠结局发生频率为19.1%,对照组为4.2%,差异具有统计学意义(χ2=29.3,P=0.000)。叶酸与Hcy 呈负相关(回归方程:Y=-0.785X+0.913,r=-0.859,P<0.05)。结论 乌鲁木齐地区女性MTHFR位点TT基因型频率与文献报道一致。叶酸浓度降低,而Hcy浓度升高,两者关系呈负相关。女性在怀孕期需进行MTHFR基因多态性检测。
Abstract:
Objective To investigate the effect of methylenetetrahydrofolate reductase(MTHFR)mutation on the concentration of folate andhomocysteine(Hcy),in addition,the correlation between folate and Hcy in TT genotypes with MTHFR polymorphism was also analyzed.Methods Total of 110 MTHFR C677 genotypes were selected as the observation group,and118 were selected as the control group according to the inclusion criteria.Thepolymorphism of MTHFR gene was detected by PCR-gold magnetic particle chromatography.The concentration of folate in serum was determined by electrochemical luminescence method,and the serum Hcy was detected by automatic biochemical analysis and circulating acid method.Results The TT genotypefrequency of MTHFR loci was 24.5%.The mean value of folate in the observationgroups was 4.9±2.6 μg/L,the mean value of Hcy was 25.9±2.9 μmol/L,and the mean value of folate in the control group were 15.5±1.9 μg/L,and the mean value of Hcy in the control group were 10.5±1.8 μmol/L.The difference between the two groups was statistically significant(t=8.356,9.835,P<0.005).The frequency of adverse pregnancy in the observation group were 19.1%,the control group was 4.2%,the difference was statistically significant(χ2=29.3,P=0.000).Folate negatively correlated with Hcy(regression equation:Y=-0.785X+0.913,r=-0.859,P<0.05).Conclusion The TT genotype frequency of female MTHFR loci in Urumqi was consistent with literature report.There was a negative correlation between folate concentrationand Hcy concentration.Female should be tested for MTHFR polymorphism during pregnancy.

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备注/Memo

备注/Memo:
作者简介:张 燕(1975-),女,大学本科,副主任检验师,研究方向:分子生物学,E-mail:1017537634@qq.com。
更新日期/Last Update: 2019-10-20